ACTIVIDAD ANTIOXIDANTE Y ANTIFÚNGICA IN VITRO DE EXTRACTOS DE CARAMBOLA (Averrhoa carambola L.)

Luis Angel Xoca-Orozco, Víctor Zamora-Gasga, Gabriela Espinosa-Alonso, Rita María Velázquez-Estrada, Ulises López-García, Sonia Sáyago-Ayerdi, Alejandra Chacón-López

Resumen


Se llevó a cabo la obtención de extractos de hoja de carambola (Averrhoa carambola L.) con el objetivo de evaluar su actividad antioxidante y capacidad antifúngica. La extracción fue asistida por ultrasonidos. Se aplicaron tres diferentes tratamientos, donde el tiempo de extracción, el ciclo de pulso y la amplitud de sonicación fueron evaluados. Una vez obtenidos los extractos a las diferentes condiciones de sonicación, se determinó para cada uno de ellos el contenido de fenoles totales y la actividad antioxidante por tres métodos: DPPH, ABTS y FRAP. Además se evaluó la actividad antifúngica de los extractos, lo cual fue determinado mediante su capacidad de inhibir el desarrollo de Colletotrichum sp en condiciones in vitro. Los resultados indican que los extractos logran una inhibición de hasta el 53% del crecimiento micelial del hongo in vitro, así como una reducción en la esporulación del fitopatógeno. Estos resultados contribuyen a la generación de evidenciapara el desarrollo de nuevas alternativas para el control de enfermedades poscosecha utilizando hojas de carambola como fuente de compuestos con capacidad antifúngica.


Palabras clave


Carambola; Ultrasonidos; Antifúngico; Colletotrichum; Poscosecha

Texto completo:

PDF

Referencias


Albu, S., E. Joyce, L. Paniwnyk, J. P. Lorimer, and T. J. Mason. 2004. “Potential for the Use of Ultrasound in the Extraction of Antioxidants from Rosmarinus officinalis for the Food and Pharmaceutical Industry.” Ultrasonics Sonochemistry 11 (3-4):261 65. https://doi.org/10.1016/j.ultsonch.2004.01.015.

Alvarez-Parrilla, Emilio, Laura A. De La Rosa, Ryszard Amarowicz, and Fereidoon Shahidi. 2011. “Antioxidant Activity of Fresh and Processed Jalapeño and Serrano Peppers.” Journal of Agricultural and Food Chemistry 59 (1):163–73. https://doi.org/10.1021/jf103434u.

Alvarez-Parrilla, Emilio, Laura A. De La Rosa, Patricia Legarreta, Laura Saenz, Joaquín Rodrigo-García, and Gustavo A. González-Aguilar. 2010. “Daily Consumption of Apple, Pear and Orange Juice Differently Affects Plasma Lipids and Antioxidant Capacity of Smoking and Non-Smoking Adults.” International Journal of Food Sciences and Nutrition 61 (4):369–80. https://doi.org/10.3109/09637480903514041.

Annegowda, H. V., Rajeev Bhat, Liong Min-Tze, A. A. Karim, and S. M. Mansor. 2012. “Influence of Sonication Treatments and Extraction Solvents on the Phenolics and Antioxidants in Star Fruits.” Journal of Food Science and Technology 49 (4). Springer-Verlag:510–14. https://doi.org/10.1007/s13197-011-0435-8.

Bazie, Setu, Amare Ayalew, and Kebede Woldetsadik. 2014. “Antifungal Activity of Some Plant Extracts against (Colletotrichum Musae ) the Cause of Postharvest Banana Anthracnose.” Plant Pathology & Microbiology 5 (2):2–5. https://doi.org/10.4172/2157-7471.1000226.

Dasgupta, P, P Chakraborty, and N N Bala. 2013. “Averrhoa Carambola: An Updated Review.” International Journal of Pharma Research & Review IJPRR 2 (27):54–63.

Diana, Cerqueira Sales María, Helber Barcellos Costa, Patrícia Machado Bueno Fernandes, José Aires Ventura, and Debora Dummer meira. 2016. “Antifungal Activity of Plant Extracts with Potential to Control Plant Pathogens in Pineapple.” Asian Paci Fi c Journal of Tropical Biomedicine 6 (1):26–31. https://doi.org/10.1016/j.apjtb.2015.09.026.

Gimbun, J, N F Ishak, N I S Muhammad, S F Pang, M A A Kadir, H Ramli, M F Abdullah, Z Giman, and Z Khadisah. 2014. “Ultrasonic Assisted Extraction Polyphenols and Antioxidant from Nigella Sativa Seed.” Journal of Engineering and Technology 5 (2):17–26.

Hirasawa, Masatomo, and Kazuko Takada. 2004. “Multiple Effects of Green Tea Catechin on the Antifungal Activity of Antimycotics against Candida Albicans.” The Journal of Antimicrobial Chemotherapy 53 (2). Oxford University Press:225–29. https://doi.org/10.1093/jac/dkh046.

Ikigai, Hajime, Taiji Nakae, Yukihiko Hara, and Tadakatsu

Shimamura. 1993. “Bactericidal Catechins Damage the Lipid Bilayer.” Biochimica et Biophysica Acta (BBA) - Biomembranes 1147 (1). Elsevier:132–36. https://doi.org/10.1016/0005-2736(93)90323-R.

Kalidindi, Narasimharaju, Nagarasanakote Venkataswamy, Nagepally Venkataramareddy, Ramaiah Nandeep, Sathyanarayana Swetha, and Bharat Kalidindi. 2015. “Antifungal and Antioxidant Activities of Organic and Aqueous Extracts of Annona Squamosa Linn . Leaves.” Journal of Food and Drug Analysis 3. Elsevier Ltd:795–802. https://doi.org/10.1016/j.jfda.2015.04.012.

Lim, Yin Sze, and Shi Ting Lee. 2013. “In Vitro Antioxidant Capacities of Star Fruit ( Averrhoa Carambola ), an Underutilised Tropical Fruit.” Journal of Biology 1 (1):21–24.

Mia, Md Masum, Mohammad S. Rahman, Kohinur Begum, Bilkis Begum, and Mohammad A. Rashid. 2007. “Phytochemical and Biological Studies of Averrhoa Carambola.” Dhaka University Journal of Pharmaceutical Sciences 6 (2):125–28.

Moreno, Silvia, Tamara Scheyer, Catalina S. Romano, and Adrián A. Vojnov. 2006. “Antioxidant and Antimicrobial Activities of Rosemary Extracts Linked to Their Polyphenol Composition.” Free Radical Research 40 (2):223–31. https://doi.org/10.1080/10715760500473834.

Pérez-Barraza, M. H., V. Vázquez-Valdivia, and J. Osuna-García. 2005. “El Cultivo Del Carambolo ( Averroha Carambola L.): Alternativa Para El Tropico Seco.” Revista Chapingo 11 (1):83–87.

Prior, Ronald L., Xianli Wu, and Karen Schaich. 2005. “Standarized Methods for the Determination of Antioxidant Capacity and Phenolics in Foods and Dietary Supplements.” Journal of Agricultural and Food Chemistry 53 (10):4290–4302. https://doi.org/10.1021/jf0502698.

Re, Roberta, Nicoletta Pellegrini, Anna Proteggente, Ananth Pannala, Min Yang, and Catherine Rice-Evans. 1999. “Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay.” Free Radical Biology and Medicine 26 (9):1231–37.

https://doi.org/10.1016/S0891-5849(98)00315-3.

Saghir, S A M, A Sadikun, K Y Khaw, and V Murugaiyah. 2013. “Star Fruit (Averrhoa Carambola L.): From Traditional Uses to Pharmacological Activities.” Boletin Latinoamericano Y Del Caribe de Plantas Medicinales Y Aromaticas 12 (3):209–19. http://www.embase.com/search/results?subaction=viewrecord&from=export&id=L368881952%5Cnhttp://www.blacpma.usach.cl/images/docs/012-003/002_revision.pdf.

Silva, Polyanna Alves, Denilson Ferreira Oliveira, Ney Taironi do Prado, Douglas Antonio de Carvaho, and Gilvane Aparecida De Carbalho Carvalho. 2008. “EVALUATION OF THE ANTIFUNGAL ACTIVITY BY PLANT EXTRACTS AGAINST Colletotrichum Gloeosporioides PENZ.” Cienc. Agro., Lavras 32 (2):420–28.

Sripanidkulchai, Bungorn, Unchalee Tattawasart, Pisamai Laupattarakasem, and Varina Wongpanich. 2002. “Anti-Inflammatory and Bactericidal Properties of Selected Indigenous Medicinal Plants Used for Dysuria.” Thai Journal of Pharmaceutical Sciences 26:33–38.

Toyoshima, Y, S Okubo, M Toda, Y Hara, and T Shimamura. 1994. “[Effect of Catechin on the Ultrastructure of Trichophyton Mentagrophytes].” Kansenshogaku Zasshi. The Journal of the Japanese Association for Infectious Diseases 68 (3):295–303. http://www.ncbi.nlm.nih.gov/pubmed/8176271.

Vilkhu, Kamaljit, Raymond Mawson, Lloyd Simons, and Darren Bates. 2008. “Applications and Opportunities for Ultrasound Assisted Extraction in the Food Industry — A Review.” Innovative Food Science & Emerging Technologies 9 (2):161–69. https://doi.org/10.1016/j.ifset.2007.04.014.

Wei, Shu-Dong, Hui Chen, Ting Yan, Yi-Ming Lin, and Hai-Chao Zhou. 2014. “Identification of Antioxidant Components and Fatty Acid Profiles of the Leaves and Fruits from Averrhoa Carambola.” LWT - Food Science and Technology 55 (1):278–85. https://doi.org/10.1016/j.lwt.2013.08.013.

Xoca-Orozco, Luis-Ángel, Esther Angélica Cuellar-Torres, Sandra González-Morales, Porfirio Gutiérrez-Martínez, Ulises López-García, Luis Herrera-Estrella, Julio Vega-Arreguín, and Alejandra Chacón López. 2017. “Transcriptomic Analysis of Avocado Hass (Persea Americana Mill) in the Interaction System Fruit-Chitosan Colletotrichum.” Frontiers in Plant Science 8 (June): 1–13. doi:10.3389/fpls.2017.00956.

Zamora-Gasga, Victor Manuel, Mitsuky Soraya Serafín-García, Jorge Alberto Sánchez-Burgos, Rita María Velázquez Estrada, and Sonia Guadalupe Sáyago-Ayerdi. 2016. “Optimization of Ultrasonic Assisted Extraction of Antioxidant Compounds from Starfruit

(Averroha Carambola L) Leaves.” Journal of Food Processing and Preservation 0:1–10. https://doi.org/10.1111/jfpp.13093.




DOI: http://dx.doi.org/10.18633/biotecnia.v20i2.608

Enlaces refback

  • No hay ningún enlace refback.