Effect of substrates on the kefir grain biomass production and antioxidant activity
DOI:
https://doi.org/10.18633/biotecnia.v24i1.1614Palabras clave:
kéfir, sustratos, actividad antioxidante, biomasa de los granos de kéfir.Resumen
El kéfir es una bebida láctea fermentada con bioactividades. Sin embargo, la biomasa de los granos de kéfir (KGB) limita su producción. En éste trabajo se evaluó el efecto de diferentes sustratos en la KGB y la actividad antioxidante (AC). Los granos de kéfir fueron inoculados (6 % w/v) en leche de cabra (GMK), GMK con azúcar morena (GMBSK), leche de vaca (CMK), y CMK con azúcar morena (CMBSK). La fermentación se realizó a 25 ± 3 °C y fue detenida a pH 4.7, aproximadamente. Las muestras se tomaron a las 0, 4 y 8 h de incubación. Las cinéticas del incremento de KGB, parámetros del crecimiento microbiano y actividad antioxidante fueron evaluadas. Los kéfires elaborados con leche de cabra presentaron los valores de AC más altos. GMBSK y GMK tuvieron 44.8 ± 1.4 % y 37.6 ± 3.6 % AC, respectivamente. GMK y GMBSK tuvieron los mayores incrementos de biomasa, 147 ± 23 % y 136 ± 7 %, respectivamente. Además, GMK tuvo la productividad de KGB más elevada (p < 0.01), 2.49 ± 0.24 g/L.h. La leche de cabra representa un sustrato alternativo con potencial para incrementar la KGB y la AC en la bebida de kéfir.
Descargas
Citas
Álvarez-Martín, P. et al. (2008) ‘Interaction between dairy yeasts and lactic acid bacteria strains during milk fermentation’, Food Control, 19(1), pp. 62–70. doi: 10.1016/j.foodcont.2007.02.003.
Bradford, M. M. (1976) ‘A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding’, Analytical Biochemistry, 72, pp. 248–254. doi: 10.1016/0003-2697(76)90527-3.
Ceballos, L. S. et al. (2009) ‘Composition of goat and cow milk produced under similar conditions and analyzed by identical methodology’, Journal of Food Composition and Analysis, 22(4), pp. 322–329. doi: 10.1016/j.jfca.2008.10.020.
Clark, S. and Mora García, M. B. (2017) ‘A 100-Year Review: Advances in goat milk research’, Journal of Dairy Science, 100(12), pp. 10026–10044. doi: 10.3168/jds.2017-13287.
Dallas, D. C. et al. (2016) ‘Peptidomic analysis reveals proteolytic activity of kefir microorganisms on bovine milk proteins’, Food Chemistry, 197, pp. 273–284. doi: 10.1016/j.foodchem.2015.10.116.
Demirhan, E. et al. (2013) ‘Influence of vitamin (B1, B6, B9, B12, C) and ions (Cu2+, Mn2+, PO4 3-) on kefir grain biomass growth’, Food Science and Biotechnology, 22(4), pp. 1007–1013. doi: 10.1007/s10068-013-0177-7.
Dermihan, E. et al. (2011) ‘A modelling study on kefir grain biomass growth : Influence of various minerals’, 64(3), pp. 402–407. doi: 10.1111/j.1471-0307.2011.00696.x.
Fatahi, A., Soleimani, N. and Afrough, P. (2021) ‘Anticancer Activity of Kefir on Glioblastoma Cancer Cell as a New Treatment’, International Journal of Food Science, pp. 1–5. doi: 10.1155/2021/8180742.
Gamba, R. R. et al. (2020) ‘Chemical, Microbiological, and Functional Characterization of Kefir Produced from Cow’s Milk and Soy Milk’, International Journal of Microbiology, pp. 1–11. doi: 10.1155/2020/7019286.
Goršek, A. and Tramšek, M. (2008) ‘Kefir grains production—An approach for volume optimization of two-stage bioreactor system’, Biochemical Engineering Journal, 42(2), pp. 153–158. doi: 10.1016/j.bej.2008.06.009.
Gradova, N. B. et al. (2015) ‘Microbial components of kefir grains as exopolysaccharide kefiran producers’, Applied Biochemistry and Microbiology, 51(9), pp. 873–880. doi: 10.1134/S0003683815090045.
Guzel-Seydim, Z. et al. (2011) ‘Effect of different growth conditions on biomass increase in kefir grains.’, Journal of dairy science, 94(3), pp. 1239–1242. doi: 10.3168/jds.2010-3349.
Hsieh, H.-H. et al. (2012) ‘Effects of cow’s and goat’s milk as fermentation media on the microbial ecology of sugary kefir grains.’, International journal of food microbiology, 157(1), pp. 73–81. doi: 10.1016/j.ijfoodmicro.2012.04.014.
Izquierdo-González, J. J. et al. (2019) ‘Proteomic analysis of goat milk kefir: Profiling the fermentation-time dependent protein digestion and identification of potential peptides with biological activity’, Food Chemistry. Elsevier, 295, pp. 456–465. doi: 10.1016/J.FOODCHEM.2019.05.178.
Kaptan, B., Kayısoglu, S. and Oksuz, O. (2015) ‘Mathematical Modeling of pH Variation as a Function of Temperature and Time in Kefir Production’, American Journal of Food Science and Nutrition Research, 2(2), pp. 57–61. Available at: http://www.openscienceonline.com/journal/fsnr.
Kilic, D., Ozel, B. and Ozbek, B. (2016) ‘Kefir grain biomass production: Influence of different culturing conditions and examination of growth kinetic models’, pp. 1–9. doi: 10.1111/jfpe.12332.
Korsak, N. et al. (2015) ‘Short communication: Evaluation of the microbiota of kefir samples using metagenetic analysis targeting the 16S and 26S ribosomal DNA fragments.’, Journal of dairy science, 98(6), pp. 3684–9. doi: 10.3168/jds.2014-9065.
Liu, J.-R. et al. (2005) ‘Antioxidative Activities of Kefir’, Asian-Aust, 18(4), pp. 567–573.
Maeda, H. et al. (2004) ‘Structural characterization and biological activities of an exopolysaccharide kefiran produced by Lactobacillus kefiranofaciens WT-2B T’, Journal of Agricultural and Food Chemistry, 52(17), pp. 5533–5538. doi: 10.1021/jf049617g.
Narendranath, N. V. and Power, R. (2005) ‘Relationship between pH and medium dissolved solids in terms of growth and metabolism of lactobacilli and Saccharomyces cerevisiae during ethanol production’, Applied and Environmental Microbiology, 71, pp. 2239–2243. doi: 10.1128/AEM.71.5.2239-2243.2005.
Official methods of analysis. Method 925.23.A.O.A.C. International. (2012) A.O.A.C. International. 19th ed. Washington, D.C.
Panchal, G., Hati, S. and Sakure, A. (2019) ‘Characterization and production of novel antioxidative peptides derived from fermented goat milk by L. fermentum’, LWT. Academic Press, 119, p. 108887. doi: 10.1016/J.LWT.2019.108887.
Papapostolou, H. et al. (2008) ‘Fermentation efficiency of thermally dried kefir’, Bioresource Technology, 99(15), pp. 6949–6956. doi: 10.1016/j.biortech.2008.01.026.
Park, Y. W. et al. (2007) ‘Physico-chemical characteristics of goat and sheep milk’, Small Ruminant Research, 68(1–2), pp. 88–113. doi: 10.1016/j.smallrumres.2006.09.013.
Re, R. et al. (1999) ‘Antioxidant activity applying an improved ABTS radical cation decolorization assay’, Free Radical Biology and Medicine, 26(9–10), pp. 1231–1237. doi: 10.1016/S0891-5849(98)00315-3.
Satir, G. and Guzel-Seydim, Z. B. (2015) ‘Influence of Kefir fermentation on the bioactive substances ofdifferent breed goat milks’, LWT - Food Science and Technology, 63(2), pp. 852–858. doi: 10.1016/j.lwt.2015.04.057.
Savastano, M. L. et al. (2020) ‘Influence of the production technology on kefir characteristics: Evaluation of microbiological aspects and profiling of phosphopeptides by LC-ESI-QTOF-MS/MS’, Food Research International, 129, p. 108853. doi: 10.1016/j.foodres.2019.108853.
Schoevers, A. and Britz, T. J. (2003) ‘Influence of different culturing conditions on kefir grain increase’, International Journal of Dairy Technology, 56, pp. 183–187. doi: 10.1046/j.1471-0307.2003.00104.x.
Tarango-Hernández, S. et al. (2015) ‘Short communication: Potential of Fresco-style cheese whey as a source of protein fractions with antioxidant and angiotensin-I-converting enzyme inhibitory activities.’, Journal of dairy science, 98(11), pp. 7635–9. doi: 10.3168/jds.2015-9388.
Ton, A. M. M. et al. (2020) ‘Oxidative Stress and Dementia in Alzheimer’s Patients: Effects of Synbiotic Supplementation’, Oxidative Medicine and Cellular Longevity, pp. 1–14. doi: 10.1155/2020/2638703.
TramŠek, M. and GorŠek, A. (2008) ‘Analysis of growth models for batch kefir grain biomass production in rc1 reaction system’, Journal of Food Process Engineering, 31(6), pp. 754–767. doi: 10.1111/j.1745-4530.2007.00187.x.
Wawrzyniak, J. et al. (2019) ‘Mathematical modelling of ethanol production as a function of temperature during lactic-alcoholic fermentation of goat’s milk after hydrolysis and transgalactosylation of lactose’, Measurement. Elsevier, 135, pp. 287–293. doi: 10.1016/J.MEASUREMENT.2018.11.070.
Yilmaz-Ersan, L. et al. (2016) ‘The Antioxidative Capacity of Kefir Produced from Goat Milk’, International Journal of Chemical Engineering and Applications, 7(1), pp. 22–26. doi: 10.7763/IJCEA.2016.V7.535.
Zajsek, K. and Gorsefe, A. (2009) ‘IChemE Modelling of batch kefir fermentation kinetics for ethanol production by mixed natural microflora’, 8(June), pp. 55–60. doi: 10.1016/j.fbp.2009.09.002.
Zulueta, A. et al. (2009) ‘Antioxidant capacity of cow milk, whey and deproteinized milk’, International Dairy Journal, 19(6–7), pp. 380–385. doi: 10.1016/j.idairyj.2009.02.003.
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
Derechos de autor 2022
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.
La revista Biotecnia se encuentra bajo la licencia Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)