CAMBIOS EN ACTIVIDAD DE TREHALASA ESTÁN ASOCIADOS A CAMBIOS EN EL ESTATUS HÍDRICO EN Selaginella Lepidophylla

  • C G Figueroa-Soto Centro de Investigación en Alimentación y Desarrollo A.C.
  • E Terán-Acuña Centro de Investigación en Alimentación y Desarrollo A.C.
  • E M Valenzuela-Soto Centro de Investigación en Alimentación y Desarrollo A.C.
Palabras clave: Tolerancia a la desecación, osmolito, osmoproteción, planta de resurrección, trehalosa.

Resumen

Selaginella lepidophylla es una planta tolerante a la desecación, conocida por su habilidad para sobrevivir largos periodos de sequía, debido a su capacidad para acumular niveles altos de trehalosa. Plantas secas de S. lepidophylla hidratadas durante 24 horas mostraron una rápida cinética de hidratación. Las plantas alcanzaron un 48 % de Contenido Relativo de Agua (CRA) a las 4 horas de hidratación. Plantas totalmente hidratadas mostraron una cinética de deshidratación menos acelerada, presentando un CRA del 50 % a las 8 horas de deshidratación. Las plantas secas mostraron actividad de trehalasa neutra y ácida. La actividad máxima de trehalasa neutra se alcanzó a un CRA de 48 %, mientras que el máximo para trehalasa ácida se encontró en plantas totalmente hidratadas. Las plantas sometidas a deshidratación durante 24 horas mostraron actividad de trehalasa neutra y baja actividad de trehalasa ácida. Se encontró un máximo de actividad de trehalasa neutra a un CRA del 85 % y del 50 % para trehalasa ácida. El patrón de actividad enzimática varió en relación con la concentración de trehalosa. Se demostró que la actividad de trehalasa cambia durante la toma y pérdida de agua y qué estos cambios se reflejan en la concentración de trehalosa en plantas de Selaginella.

Citas

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Publicado
2014-04-30
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