Partición de proteína de lactosuero en un sistema de extracción acuosa en dos fases
DOI:
https://doi.org/10.18633/biotecnia.v23i3.1263Palabras clave:
ATPE proteína/(NH4)2SO4, proteínas del suero de queso, inspección de alto rendimiento, separación de lactosaResumen
El lactosuero es un subproducto de la fabricación de queso que contiene proteínas que pueden recuperarse, pero están muy diluidas y en mezcla con lactosa, lo que limita su aprovechamiento. La técnica de extracción acuosa en dos fases (ATPE, por sus siglas en inglés) es un procedimiento con
potencial para separar proteínas. El objetivo fue evaluar el comportamiento de un sistema de extracción acuosa proteína-(NH4)2SO4 aplicado para la recuperación de proteínas de lactosuero, atendiendo la reducción de lactosa. Se construyó un diagrama binodal de fases mediante una técnica de inspección de alto rendimiento. Se prepararon soluciones de (NH4)2SO4 en concentraciones entre 22 y 40 % usando lactosuero como disolvente. El volumen de la fase superior de los sistemas fue menor que el de la inferior, pero el incremento de (NH4)2SO4 causó aumento gradual de la fase superior. La proteína de lactosuero se concentró en mayor cantidad en la fase superior alrededor del 80 %, en tanto que más del 79 % de la lactosa se separó en la fase inferior. La técnica de ATPE tiene potencial para separar proteínas del lactosuero y la mejor alternativa se basa en sistemas con 34 % de (NH4)2SO4.
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