Efecto dosis-dependiente de la CHH-B1 recombinante sobre la capacidad osmorreguladora y la expresión de la Na+/K+-ATPasa en camarones Litopenaeus vannamei sometidos a ablacion bilateral del pedúnculo ocular

Autores/as

DOI:

https://doi.org/10.18633/biotecnia.v23i3.1441

Palabras clave:

Litopenaeus vannamei, camarón, hormona hiperglucémica de crustáceos, capacidad os, Na /k ATPasa

Resumen

La osmorregulación en crustáceos está bajo control neuroendócrino por las hormonas hiperglucémicas de crustáceos (CHHs), las cuales han mostrado modificar las concentraciones de agua e iones en diversas especies. Estudios previos hechos por nuestro grupo sugieren que la CHH variante B1 (CHH-B1) tiene efectos en las respuestas osmorreguladoras del camarón blanco Litopenaeus vannamei. Para una mejor compresión sobre los mecanismos de acción moleculares de CHH-B1 en osmoregulación, en este trabajo, establecimos el efecto dosis-dependiente de la CHH-B1 recombinante sobre la expresión génica de la Na+/K+-ATPasa en branquias, en contraste con lo cambios en la capacidad osmorreguladora (CO) de camarones bilateralmente ablacionados del pedúnculo ocular bajo condiciones hiper-osmóticas. Los resultados indican que CHH-B1 regula la OC de camarones durante la hipo-regulación mediante la modulación de la NKA a nivel transcripcional. Nuestros resultados sugieren que CHH tiene una participación directa en el control de los mecanismos de regulación osmo-iónica, no solo en L. vannamei sino en crustáceos en general.

Biografía del autor/a

Laura Camacho-Jiménez, Centro de Investigación Científica y de Educación Superior de Ensenada

Departamento de Biotecnología Marina

 

Dra

Fernando Diaz, Centro de Investigación Científica y de Educación Superior de Ensenada

Departamento de Biotecnología Marina

Dr.

Edna Sánchez-Castrejón, Centro de Investigación Científica y de Educación Superior de Ensenada

Departamento de Biotecnología Marina

Dra.

Ariana Montiel-Arzate, Centro de Investigación Científica y de Educación Superior de Ensenada

Departamento de Biotecnología Marina

Maestra en Ciencias de la Vida

Citas

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2021-09-08

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