Isolation and characterization of one autochthonous Beauveria sp. isolate from Cuban fields

Autores/as

  • Alianna Machín Suárez Universidad Agraria de la Habana “Fructuoso Rodríguez Pérez”
  • Elio Minel del Pozo Núñez Universidad Agraria de la Habana “Fructuoso Rodríguez Pérez”
  • Irma García Cruz Universidad Agraria de la Habana “Fructuoso Rodríguez Pérez”
  • Eduardo Fidel Héctor Ardisana Instituto de Posgrado, Universidad Técnica de Manabí
  • Yoannis Domínguez Universidade Estadual Paulista (UNESP), Instituto de Biociências
  • Sergio Guerrero-Morales Facultad de Ciencias Agrícolas y Forestales, Universidad Autónoma de Chihuahua
  • Jesús Alicia Chávez Medina Instituto Politécnico Nacional, CIIDIR Unidad Sinaloa
  • César Marcial Escobedo Bonilla Instituto Politécnico Nacional, CIIDIR Unidad Sinaloa
  • Sandra Pèrez Àlvarez Facultad de Ciencias Agricolas y Forestales

DOI:

https://doi.org/10.18633/biotecnia.v24i1.1553

Palabras clave:

Aislado autóctono, entomopatógeno, actividad enzimática, morfología, control de plagas

Resumen

El género Beauveria comprende un grupo cosmopolita teleomórfico y anamórfico de hongos entomopatógenos. El objetivo de esta investigación fue caracterizar el nuevo aislado de Beauveria spp. en comparación con la cepa comercial LBB-1. El aislamiento se obtuvo de Atta insularis y adultos del barrenador del café, respectivamente. Se cultivó en medio sólido SDAY enriquecido con 1% de extracto de levadura. Se determinó la eficacia biológica del aislado sobre C. formicarius. El ADN se aisló usando el método CTAB y se usó un espaciador transcrito interno (ITS1, ITS4) como cebadores para la amplificación. La secuencia consenso obtenida se depositó en GenBank (MK246940) y se utilizó para análisis filogenético. Este aislado mostró diferencias en sus características morfológicas; sin embargo, mostró una baja variabilidad genética con respecto a los aislamientos mundiales de B. bassiana. En cuanto a su eficacia biológica, el aislado demostró ser virulento sobre los adultos fitófagos, alcanzando el 50% de mortalidad. Se identificaron cuatro clados y la topografía politómica recuperada para el clado 1 indica una alta similitud genética (99-96%) con Beauveria bassiana de varios países. La caracterización morfológica y molecular permitió identificar con precisión la especie del nuevo aislado. La especie identificada es otra posibilidad para el manejo integrado de plagas en Cuba y México.

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Publicado

2022-03-04

Cómo citar

Machín Suárez, A. ., del Pozo Núñez, E. M. ., García Cruz, I. ., Héctor Ardisana, E. F. ., Domínguez, Y. ., Guerrero-Morales, S. ., Chávez Medina, J. A. ., Escobedo Bonilla, C. M. ., & Pèrez Àlvarez, S. (2022). Isolation and characterization of one autochthonous Beauveria sp. isolate from Cuban fields. Biotecnia, 24(1), 142–150. https://doi.org/10.18633/biotecnia.v24i1.1553

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