Isolation and characterization of one autochthonous Beauveria sp. isolate from Cuban fields
DOI:
https://doi.org/10.18633/biotecnia.v24i1.1553Palabras clave:
Aislado autóctono, entomopatógeno, actividad enzimática, morfología, control de plagasResumen
El género Beauveria comprende un grupo cosmopolita teleomórfico y anamórfico de hongos entomopatógenos. El objetivo de esta investigación fue caracterizar el nuevo aislado de Beauveria spp. en comparación con la cepa comercial LBB-1. El aislamiento se obtuvo de Atta insularis y adultos del barrenador del café, respectivamente. Se cultivó en medio sólido SDAY enriquecido con 1% de extracto de levadura. Se determinó la eficacia biológica del aislado sobre C. formicarius. El ADN se aisló usando el método CTAB y se usó un espaciador transcrito interno (ITS1, ITS4) como cebadores para la amplificación. La secuencia consenso obtenida se depositó en GenBank (MK246940) y se utilizó para análisis filogenético. Este aislado mostró diferencias en sus características morfológicas; sin embargo, mostró una baja variabilidad genética con respecto a los aislamientos mundiales de B. bassiana. En cuanto a su eficacia biológica, el aislado demostró ser virulento sobre los adultos fitófagos, alcanzando el 50% de mortalidad. Se identificaron cuatro clados y la topografía politómica recuperada para el clado 1 indica una alta similitud genética (99-96%) con Beauveria bassiana de varios países. La caracterización morfológica y molecular permitió identificar con precisión la especie del nuevo aislado. La especie identificada es otra posibilidad para el manejo integrado de plagas en Cuba y México.
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