Effect of ion and protein concentration of Ps19, a shell protein from Pteria sterna, on calcium carbonate polymorph

Calcium carbonate polymorphs in Concha Nácar

Authors

DOI:

https://doi.org/10.18633/biotecnia.v25i2.1885

Keywords:

Mollusk, shell protein, calcium carbonate, crystallization, nacre

Abstract

Calcium carbonate is present in many biological structures such as bivalve shell, which is composed mainly of two CaCO3 polymorphs: calcite and aragonite. However, exist other forms of calcium carbonate like vaterite and amorphous calcium carbonate (ACC) that are not commonly reported. Polymorph selection is influenced by salt concentration, cofactor ions, and the presence of shell matrix proteins (SMPs) which regulates calcium carbonate deposition, among other factors. In this study, calcium carbonate crystallization in vitro of four different saline solutions at two molarities was evaluated with increased concentrations of the Ps19 protein, an insoluble extracted protein from the shell of Pteria sterna, previously described as a promotor of aragonite platelet crystallization. In vitro crystallizations showed that Ps19 is capable to induce aragonite and calcite deposition in a dose-dependent manner, but also vaterite under ciertan conditions, acting as a promoter and inhibitor of crystallization. The results contribute to understand how Ps19 control precipitation of calcium polymorphs in the growth of the prismatic and nacre layer of the shell of P. sterna.

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Author Biographies

R Arroyo-Loranca, Centro de Investigaciones Biológicas del Noroeste, S.C.

Biologist from the Faculty of Sciences of the National Autonomous University of Mexico (2009); Master of Science in Marine Ecology from CICESE (2013) Campus Ensenada; Doctor of Science in the Use, Management and Preservation of Natural Resources from CIBNOR (2020).

C Rivera, Consejo Nacional de Ciencia y Tecnologia-Centro de Investigaciones Biológicas del Noroeste, S.C.

Biotechnological Engineer with a specialty in food from the Technological Institute of Sonora, Master's and Doctorate in the Use, Management and Preservation of Natural Resources at CIBNOR. He did two postdoctoral stays, one of 4 and a half years at Florida International University in the Insect Physiology Laboratory, studying the juvenile hormone pathway of the dengue mosquito A. aegypti. The second postdoctoral stay, of one year, was carried out at CIBNOR, in the Oxidative Stress laboratory, studying the effect of oxidative stress on cell cultures of humans and marine mammals. He is currently a CONACyT professor attached to CIBNOR since 2016 and an Associate Researcher at Florida International University since 2017. At CIBNOR he is part of the Molecular Genetics Laboratory associated with the Fisheries Program (PEP) and is also a content manager at the Distance Education Unit (UED) of the Postgraduate. He is SNI I since 2015 and his current line of research is Marine Biotechnology and Physiology of marine organisms within the PEP and Distance Education in Mexico within the UED.

L Hernández Adame, Consejo Nacional de Ciencia y Tecnologia-Centro de Investigaciones Biológicas del Noroeste, S.C.

Dr Hernández has a PhD in Engineering and Materials Science from the Faculty of Chemical Sciences of the UASLP (Mexico). He is currently a Researcher in the Researchers for Mexico-CONACYT program assigned to the Center for Biological Research of the Northwest (CIBNOR) in La Paz, BCS. His research is focused on developing biomaterials and nanotechnology applied to health, nutrition and pathogen control in plants and animals. Dr Hernández has conducted research stays at different Centers and Universities, including BUAP, UASLP, UAZ, UTSA-United States and Université Paris Sud-France. He has published more than 25 scientific articles and directed ten theses for obtaining a degree, including two doctoral projects, three master's degrees and five bachelor's degrees. He is a member of the SNI level 1 and a founding member of the Nanotechnology and Microbial Biocontrol group of CIBNOR.

AA Cruz Villacorta, Centro de Investigaciones Biológicas del Noroeste, S.C.

Technician specialized in electronics and microelectronics since 1991. Architectural Draftsman. Specialties: Electron Microscopy, Microanalysis, Confocal Microscopy, Chromatography (liquid, gases, thin layer), micro electrophoresis. Design and assembly of new methodologies; operation, maintenance, repair, and training in analytical instrumentation in general; development of separation techniques and processes. Scanning Electron Microscopy, preparation of specimens, advice for the different sample fixation procedures; and X-ray microanalysis. Participation in more than twenty projects as responsible and participant, coauthor in scientific articles, book chapters and conference proceedings.

JL Rodríguez López, Instituto Potosino de Investigación Científica y Tecnológica

.

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Published

2023-05-22

How to Cite

Arroyo Loranca, R. G., Rivera Pérez, C., Hernández Adame, L., Cruz Villacorta, A. A., Rodríguez López, J. L., & Hernández Saavedra, N. Y. (2023). Effect of ion and protein concentration of Ps19, a shell protein from Pteria sterna, on calcium carbonate polymorph : Calcium carbonate polymorphs in Concha Nácar. Biotecnia, 25(2), 136–145. https://doi.org/10.18633/biotecnia.v25i2.1885

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Vol. 25 No. 2 (2023): May - August

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