Monitoreo de la microbiota del hepatopáncreas asociada a genes de toxinas pirABVP en Penaeus vannamei
Monitoreo de la microbiota del hepatopáncreas
DOI:
https://doi.org/10.18633/biotecnia.v26.2181Palabras clave:
microbiota, AHPND, Vibrio, Secuenciación de ultima generacion, camaronResumen
La producción acuícola de camarón se ha expandido rápidamente en las últimas décadas, convirtiéndose en una importante actividad económica a nivel mundial. Sin embargo, algunas infecciones bacterianas y virales son un gran desafío para esta actividad. La Necrosis Hepatopancreática Aguda (AHPND) es una enfermedad bacteriana que afecta a los estanques de granjas camaroneras y se presenta durante los primeros 30 días después de la siembra en estanques de camarones, causando una mortalidad del 70-100%. La microbiota tiene un papel importante en la producción del camarón y el control de enfermedades. En el presente estudio, los genes pirA y pirB fueron amplificados por PCR para confirmar tanto organismos positivos como negativos a AHPND en hepatopáncreas de camarones. Posteriormente se secuenciaron las regiones hipervariables ribosómicas (ARNr) V3-V4 del gen 16S utilizando la plataforma MiSeq. Los resultados demostraron una microbiota diversa en el grupo negativo a AHPND, compuesto principalmente por filos Proteobacteria, Firmicutes y Actinobacteria. Además, Proteobacteria fue el filo dominante en el hepatopáncreas infectado con AHPND, mientras que Firmicutes tendió a disminuir. Vibrio fue el género más abundante, prevaleciendo sobre algunos géneros como Corynebacterium, Weissella, Lactobacillus, Photobacterium y Geobacillus. Los resultados sugieren que la enfermedad de AHPND causa disbiosis en la microbiota hepatopancreática, y el filo Firmicutes podría requerir ser restaurado en dicho escenario.
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Dabu, I.M., Lim, J.J., Arabit, P.M.T. y Orense, S.J.A.B. 2015. The first record of acute hepatopancreatic necrosis disease in the Philippines. Aquaculture Research, 48: 792-799. doi: 10.1111/are.12923
Das, S., Ward, R. y Burke, C. 2008. Prospects of using marine actinobacteria as probiotics in aquaculture. Applied Microbiology and Biotechnology, 81: 419-429. doi: 10.1007/s00253-008-1731-8
Dhar, A.K., Piamsomboon, P., Aranguren-Caro, L.F., Kanrar, S., Adami, R. y Juan, Y.S. 2019. First report of acute hepatopancreatic necrosis disease (AHPND) occurring in the USA. Diseases of Aquatic Or-ganisms, 132: 241-247. doi: 10.3354/dao03330
Duan, Y., Wang, Y., Liu, Q., Zhang, J. y Xiong, D. 2019. Changes in the intestine barrier function of Li-topenaeusvannamei in response to pH stress. Fish and Shellfish Immunology, 88: 142-149. doi: 10.1016/j.fsi.2019.02.047
De Paiva Maia, E., Alves Modesto, G., Otavio Brito, L., Olivera, A., Vasconcelos, T.C. y Gesteira, F. 2013. Effect of a commercial probiotic on bacterial and phytoplankton concentration in intensive shrimp farming (Litopenaeusvannamei) recirculation systems. Latin American Journal of Aquatic Research, 41: 126-137. doi: 10.3856/vol41-issue1-fulltext-10
Food and Agriculture Organization (FAO). 2013. Technical workshop on Early Mortality Syndrome (EMS) or Acute Hepatopancreatic Necrosis Syndrome (AHPNS) of cultured shrimp (under TCP/VIE/3304). FAO/MARD. [https://www.fao.org/3/i3422e/i3422e.pdf]. Reviewed: June 15, 2020.
Gainza, O., Ramírez, C., Ramos, A.S. y Romero, J. 2017. Intestinal microbiota of white shrimp Penaeus vannamei under intensive cultivation conditions in Ecuador. Microbial Ecology, 75: 562-568. doi: 10.1007/s00248-017-1066-z
Garibay-Valdez, E., Martínez-Porchas, M., Calderón, K., Vargas-Albores, F., Gollas-Galván, T. y Martínez-Córdova, L. 2020. Taxonomic and functional changes in the microbiota of the white shrimp (Litopenaeusvannamei) associated with postlarval ontogenetic development. Aquaculture, 518: 734842.
Han, J.E., Choi, S-K., Han, S-H. y Lee, S.C. 2020. Genomic and histopathological characteristics of Vibrio parahaemolyticus isolated from an acute hepatopancreatic necrosis disease outbreak in Pacific white shrimp (Penaeus vannamei) cultured in Korea. Aquaculture, 524: 735284. doi: 10.1016/j.aquaculture.2020.735284
Han, J., Tang, K., Tran, L. y Lightner, D. 2015a.Genotyping of virulence plasmid from Vibrio parahaemo-lyticus isolates causing acute hepatopancreatic necrosis disease in shrimp. Diseases of Aquatic Or-ganisms, 115: 245-251. doi: 10.3354/dao02906
Han, J., Tang, K., Tran, L. y Lightner, D. 2015b. Photorhabdus insect-related (Pir) toxin-like genes in a plasmid of Vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (AHPND) of shrimp. Diseases of Aquatic Organisms, 113: 33-40. doi: 10.3354/dao02830.
Holt, C., Bass, D., Stentiford, G. y van der Giezen, M. 2021. Understanding the role of shrimp gut micro-biome in health and disease. Journal of Invertebrate Pathology, 186. doi: 10.1016/j.jip.2020.107387.
Hong, X., Lu, L. y Xu, D. 2015. Progress in research on acute hepatopancreatic necrosis disease (AHPND). Aquaculture International, 24: 577-593. doi: 10.1007/s10499-015-9948-x
Illumina. 2013. 16S Metagenomic sequencing library preparation. [https://support.illumina.com/downloads/16s_metagenomic_sequencing_library_preparation.htm]. Reviewed: November 10, 2021.
Kondo, H., Tinwongger, S., Proespraiwong, P., Mavichak, R., Unajak, S. y Nozaki, R. 2014. Draft ge-nome sequences of six strains of Vibrioparahaemolyticus isolated from early mortality syn-drome/acute hepatopancreatic necrosis disease shrimp in Thailand. Genome Announcements, 2: 1-2. doi: 10.1128/genomeA.00221-14
Kondo, H., Van, P.T. y Dang, L.T. 2015. Draft genome sequence of non-Vibrio parahaemolyticus acute diseased shrimp in Vietnam. Genome Announcements, 33: e00978-15. doi: 10.1128/genomeA.00978-15
Leyton, Y. y Riquelme, C. 2008. Vibrios in the marine coastal systems. Revista de Biología Marina y Oceanografía, 43: 441-456. doi: 10.4067/S0718-19572008000300004
Li, E., Xu, C., Wang, X., Wang, S. y Zhao, Q. 2018. Gut microbiota and its modulation for healthy farming of Pacific white shrimp Litopenaeusvannamei. Reviews in Fisheries Science y Aquaculture, 26: 381-399. doi: 10.1080/23308249.2018.1440530
Liu, L., Xiao, J., Zhang, M. y Zhu, W. 2018. A Vibrio owensii strain as the causative agent of AHPND in cultured shrimp, Litopenaeusvannamei. Journal of Invertebrate Pathology, 153: 156-164. doi: 10.1016/j.jip.2018.02.005
Martin-Antonio, B., Manchado, M., Infante, C., Zerolo, R. y Ñabella, A. 2007. Intestinal microbiota varia-tion in Senegalese sole (Solea senegalensis) under different feeding regimes. Aquaculture Research, 38: 1213-1222. /doi: 10.1111/j.1365-2109.2007. 01790.x
Moriarty, D.J.W. 1997. The role of microorganisms in aquaculture ponds. Aquaculture, 151: 333-349. doi: 10.1016/S0044-8486(96)01487-1
Qi, Z., Zhang, X., Boon, N. y Bossier, P. 2009. Probiotics in aquaculture of China - Current state, problems, and prospect. Aquaculture, 290: 15-21. doi: 10.1016/j.aquaculture.2009.02.012
Ruiz-Zarzuela, I., Pérez-Sánchez, T., Blas, I. y Balcázar, J.L. 2013. Probiotics in aquaculture: a current as-sessment. Reviews in Aquaculture 6:1-14.
Rungrassamee, W., Klanchui, A., Maibunkaew, S. y Karoonuthaisiri, N. 2016. Bacterial dynamics in intes-tines of the black tiger shrimp and the Pacific white shrimp during Vibrioharveyi exposure. Journal of Invertebrate Pathology, 133: 12-19. doi: 10.1016/j.jip.2015.11.004
Rungrassamee, W., Klanchui, A., Maibunkaew, S., Chaiyapechara, S., Jiravanichpaisal, P. y Karoonutha-isiri, N. 2014. Characterization of intestinal bacteria in wild and domesticated adult black tiger shrimp (Penaeus monodon). Plos One, 9: e91853. doi: 10.1371/journal.pone.0091853
Salipante, S.J., Sengupta, D.J., Rosenthal, C., Costa, G., Spangler, J., Sims, E.H. y Hoffman, N.G. 2013. Rapid 16S rRNA next-generation sequencing of polymicrobial clinical samples for diagnosis of com-plex bacterial infections. Plos One, 8: e65226. doi: 10.1371/journal.pone.0065226
Sirikharin, R., Taengchaiyaphum, S., Sritunyalucksana, K., Thitamadee, S., Flegel, T.W. y Mavichak, R. 2014. A new and improved PCR method for detection of AHPND bacteria. [https://enaca.org/?id=96]. Reviewed: February 8, 2019.
Sousa, L.G., Cuartas, E.I. y Petriella, A.M. 2005. Fine structural analysis of the epithelial cells in the hepatopancreas of Palaemonetesargentinus (Crustacea, Decapoda, Caridea) in intermoult. Biocell, 29: 25-31.
Staley, C., Unno, T., Goulz, T.J. y Jarvis, B. 2013. Application of Illumina next-generation sequencing to characterize the bacterial community of the Upper Mississippi River. Journal of Applied Microbiolo-gy, 115: 1147-1158. doi: 10.1111/jam.12323
Sung, H.H., Hsu, S.F., Chen, C.K., Ting, Y.Y. y Chao, W.L. 2001. Relationships between disease out-break in cultured tiger shrimp (Penaeus monodon) and the composition of Vibrio communities in pond water and shrimp hepatopancreas during cultivation. Aquaculture, 192: 101-110. doi: 10.1016/S0044-8486(00)00458-0
Tang, K.F.J., Bondad-Reantaso, M.G., Arthur, J.R. y MacKinnon, B. 2020. Shrimp acute hepatopancreatic necrosis disease strategy manual. FAO Fisheries and Aquaculture Circular 1190. FAO, Rome.
Trabal, N., Mazón-Suástegui, J.M., Vázquez-Juárez, R., Ascencio-Valle, F. y Romero. 2014. Changes in the composition and diversity of the bacterial microbiota associated with oysters (Crassostrea cor-teziensis, Crassostrea gigas, and Crassostrea sikamea) during commercial production. FEMS Mi-crobiology Ecology, 88: 69-83.doi: 10.1111/1574-6941.12270
Tran, L., Nunan, L., Redman, R.M., Mohney, L.L., Pantoja, C.R., Fitzsimmons, K. y Lightner. 2013. De-termination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp. Diseases of Aquatic Organisms, 105: 45-55. doi: 10.3354/dao02621
Tran, L., Redman, R.M. y Lightner, D.V. y Fitzsimmons, K. 2013. EMS/AHPNS: Infectious disease caused by bacteria. Global Aquaculture Advocate. [https://www.globalseafood.org/advocate/ems-ahpns-infectious-disease-caused-by-bacteria/]. Re-viewed: September 13, 2021.
Van Hai, N. y Fotedar, R. 2010. A review of probiotics in shrimp aquaculture. Journal of Applied Aqua-culture, 22: 251-266. doi: 10.1080/10454438.2010.500597
Vargas-Albores, F., Martínez-Porchas, M., Arvayo, M.A. y Villalpando-Canchola, E. 2016. Immuno-physiological response of Pacific white shrimp exposed to a probiotic mixture of proteobacteria and firmicutes in farm conditions. North American Journal of Aquaculture, 78: 193-202. doi: 10.1080/15222055.2016.1167797
Velazquez-Roman, J., León-Sicairos, N., Flores-Villaseñor, H., Villafaña-Rauda, S. y Canizalez-Roman, A. 2012. Association of pandemic Vibrio parahaemolyticus O3:K6 present in the coastal environment of northwest Mexico with cases of recurrent diarrhea between 2004 and 2010. Applied and Environ-mental Microbiology, 78: 1794-1803. doi: 10.1128/AEM.06953-11
Větrovský, T. y Baldrian, P. 2013. The variability of the 16S rRNA gene in bacterial genomes and its con-sequences for bacterial community analyses. Plos One, 8: 1-10. doi: 10.1371/journal.pone.0057923
Vogt, G. 2019. Functional cytology of the hepatopancreas of decapod crustaceans. Journal of morphology, 280: 1405-1444. doi: 10.1002/jmor.21040
Wang, W., Wu, X., Liu, Z. Zheng, H. y Cheng, Y. 2014. Insights into Hepatopancreatic Functions for Nu-trition Metabolism and Ovarian Development in the Crab Portunus trituberculatus: Gene Discovery in the Comparative Transcriptome of Different Hepatopancreas Stages. PLOS ONE, 9 (1): e84921- doi: 10.1371/journal.pone.0084921
Ward, N.L., Steven, B. y Penn, K. 2009. Characterization of the intestinal microbiota of two Antarctic no-tothenioid fish species. Extremophiles, 13: 679-685. doi: 10.1007/s00792-009-0252-4
Weinstock, M.A. 2013. Epidemiology and UV exposure. Journal of Investigative Dermatology, 133: 11-4. doi: 10.1038/jid.2013.248
Xiong, J., Dai, W. y Li, C. 2016. Advances, challenges, and directions in shrimp disease control: the guide-lines from an ecological perspective. Applied Microbiology and Biotechnology, 100: 6947-6954. doi: 10.1007/s00253-016-7679-1
Yang, Y., Chen, I., Lee, C. y Chen, C. 2014. Draft genome sequences of four strains of Vibrio parahaemo-lyticus, three of which cause early mortality syndrome/acute. Genome, 2: 2-3. doi: 10.1128/genomeA.00816-14
Zhang, M., Sun, Y., Liu, Y. y Qiao, F. 2016. Response of gut microbiota to salinity change in two euryha-line aquatic animals with reverse salinity preference. Aquaculture, 454: 72-80. doi: 10.1016/j.aquaculture.2015.12.014
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