Actividad antioxidante y antimicrobiana in vitro e in silico de extractos etanólicos de hojas de Cnidoscolus chayamansa

Autores/as

  • Karla Y. Posada-Mayorga Departamento de Salud, El Colegio de la Frontera Sur-Villahermosa
  • Jorge C. Ruíz-Ruíz Escuela de Nutrición, Universidad Anáhuac Mayab
  • Zendy Evelyn Olivo Vidal Departamento de Salud, El Colegio de la Frontera Sur-Villahermosa
  • Carlos A. Lobato-Tapia Universidad Politécnica Metropolitana de Puebla
  • Neith A. Pacheco-López Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C.
  • Iván E. Herrera-Pool Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C.
  • Cesar A. Irecta Najera Departamento de Salud, El Colegio de la Frontera Sur-Villahermosa
  • Xariss Miryam Sánchez Chino CONACYT-El Colegio de la Frontera Sur

DOI:

https://doi.org/10.18633/biotecnia.v26.2233

Palabras clave:

hojas de chaya, compuestos fenólicos, docking molecular, actividad antioxidante, actividad antibacteriana

Resumen

Las hojas de Cnidoscolus chayamansa ―utilizadas en la gastronomía y la medicina tradicional en México― son ricas en compuestos fenólicos que pueden tener actividad antioxidante y antimicrobiana. En este estudio evaluamos la actividad antioxidante in vitro y la actividad antibacteriana in silico de extractos etanólicos de hojas de C. chayamansa obtenidos por ultrasonicación. El contenido de compuestos fenólicos fue de 14.37 mg GAE ml-1. Se lograron identificar compuestos como nucleósido de guanosina, ácido cumárico y los derivados de kaempferol mediante UPLC-PDA-ESI-MS. Los extractos tuvieron actividad antioxidante por medio de la quelación del Cu2+ (65.53%) y el poder reductor del Fe3+ (69.59%). Aunque no se encontró actividad antibacteriana contra E. coli y S. aureus, por medio de la inhibición de crecimiento en disco, el análisis in silico reveló que los compuestos fenólicos aislados modifican las vías de señalización esenciales para la supervivencia de las bacterias estudiadas.

 

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Bautista-Robles, V., Guerrero-Reyes, G., Sánchez-Torres, G.I., Parada-Luna, F., Barrios-Gutiérrez, J.J., Vázquez-Cerero, D., Martínez-Sala, G., Siliceo-Murrieta, J.I., González-Villoria, R.A.M., Keita, H. 2020. Cnidoscolus aconitifolius: therapeutic use and phytochemical properties. Literature review. Revista de la Facultad de Medicina. 68(3):446–452. doi:/10.15446/revfacmed.v68n3.75184.

Belabdelli, F., Bekhti, N., Piras, A., Benhafsa, FM., Ilham, M., Adil, S., Anes, L. 2022. Chemical composition, antioxidant and antibacterial activity of Crataegus monogyna leaves extracts. Natural Products Research. 36(12):3234-3239. https://doi.org/10.1080/14786419.2021.1958215

Biasi-Garbin, R.P., Fabris, M., Morguette, A.E.B., Andriani, G.M., Cabral, W.R., Pereira, P.M., Brito, T.O., Macedo Jr, F., Da Silva-Lima, C.H., Nakamura, C.V., Pinge-Filho, P., Tavares, E.R., Yamauchi L.M., Bispo, M.L.F. ,Yamada-Ogatta, S.F. 2022. In Vitro Antimicrobial Screening of Benzoylthioureas: Synthesis, Antibacterial Activity toward Streptococcus agalactiae and Molecular Docking Study. ChemistrySelect, 7(34), e202202117. Doi: 10.1002/slct.202202117

Bourne, C.R., Barrow, E.W., Bunce, R.A., Bourne, P.C., Berlin, K.D., Barrow, W.W. 2010. Inhibition of antibiotic-resistant Staphylococcus aureus by the broad-spectrum dihydrofolate reductase inhibitor RAB1. Antimicrobial Agents and Chemotherapy. 54(9): 3825-3833. Doi: 10.1128/AAC.00361-10

Capitani, M.I., Matus-Basto, A., Ruiz-Ruiz, J.C., Santiago-García, J.L., Betancur-Ancona, D.A., Nolasco, S.M., Tomás, M.C., Segura-Campos, M.R. 2016. Characterization of biodegradable films based on Salvia hispanica L. protein and mucilage. Food Bioprocess Technology. 9(8):1276–1286. doi:10.1007/s11947-016-1717-y.

Chemat, F., Rombaut, N., Sicaire, A.G., Meullemiestre, A., Fabiano-Tixier, A.S., Abert-Vian, M. 2017. Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrasonics Sonochemistry. 34:540–560. doi:10.1016/j.ultsonch.2016.06.035.

De Lano, W.L. 2002. Pymol: an open-source molecular graphics tool, CCP4 Newsletter. On Protein Crys-tallography. 40:82–92.

Eberhardt, J., Santos-Martins, D., Tillack, A., Forli, S. 2021. AutoDock Vina 1.2.0: New Docking Methods, Expanded Force Field, and Python Bindings. Journal of Chemical Information and Modeling. 3891-3898, 61(8). DOI: 10.1021/acs.jcim.1c00203

Elizabeth, H., Channamma, G.M., Gnanasekaran, D., Karkera, R., Durairai G, and Kumar S. 2023. In-vitro antibacterial activity of Cnidoscolus chayamansa leaves extract on selective bacterial strain . World journal of pharmacy and pharmaceutical sciences, doi:10.20959/wjpps202310-25786

Fois, B., Skok, Ž., Tomašič, T., Ilaš, J., Zidar, N., Zega, A., Peterlin Mašič, L., Szili, P., Draskovits, G., Nyerges, A., Pál, C., Kikelj, D. 2020. Dual Escherichia coli DNA gyrase A and B inhibitors with antibacterial activity. ChemMedChem, 15(3), 265-269. DOI: 10.1002/cmdc.201900607

Fu, X., Belwal, T., Cravotto, G., Luo, Z. 2020. Sono-physical and sono-chemical effects of ultrasound: Primary applications in extraction and freezing operations and influence on food components. Ultrasonics Sonochemistry. 60:104726. doi:10.1016/j.ultsonch.2019.104726.

Fukumoto, L.R., Mazza, G. 2000. Assessing antioxidant and prooxidant activities of phenolic compounds. Journal of Agricultural and Food Chemistry. 48(8):3597–3604. https://doi.org/10.1021/jf000220w.

Garcia, C., Blesso, C. N. 2021. Antioxidant properties of anthocyanins and their mechanism of action in atherosclerosis. Free Radical Biology and Medicine, 172, 152-166. doi: 10.1016/j.freeradbiomed.2021.05.040

Gutiérrez-Rebolledo, G.A., Pérez-González, M.Z., Jiménez-Arellanes, M.A. 2016. Importancia nutricional, farmacológica y química de la chaya (Cnidoscolus chayamansa). Revisión bibliográfica. Revista de Temas Ciencia y Tecnologia. 20(60):43–56.

Guzmán, E.L., González, J.C.C., Flores, M.C., Carrillo, A.S., Pescador, M.G.N., Cruz F.J.M. 2020. Effect on hyperglycemia and pancreas cells of chaya aqueous extracts from two different regions in streptozotocin-induced diabetes rats. Brazilian Journal of Pharmaceutical Sciences 56:1–5. doi:10.1590/S2175-97902019000418782.

Hanwell, M., Curtis, D., Lonie, D., Vandermeerschd, T., Zurek, E., Hutchison, G. 2012. Avogadro: An advanced semantic chemical editor, visualization, and analysis platform. Journal of Cheminformatics. 1-17, 4(8).

Hartmann, M., Lindhorst, T.K. 2011. The bacterial lectin FimH, a target for drug discovery–carbohydrate inhibitors of type 1 fimbriae‐mediated bacterial adhesion. European Journal of Organic Chemistry 2011(20‐21), 3583-3609. https://doi.org/10.1002/ejoc.201100407

He, J., Qiao, W., An, Q., Yang, T., Luo, Y. 2020. Dihydrofolate reductase inhibitors for use as antimicrobial agents. European Journal of Medicinal Chemistry. 195, 112268. DOI: 10.1016/j.ejmech.2020.112268

Hazafa, A., Rehman, K.U., Jahan, N., Jabeen, Z. 2020. The role of polyphenol (flavonoids) compounds in the treatment of cancer cells. Nutrition and Cancer. 72(3):386-397. doi: 10.1080/01635581.2019.1637006.

Herrera-Pool, E., Ramos-Díaz, A.L., Lizardi-Jiménez, M. A., Pech-Cohuo, S., Ayora-Talavera, T., Cuevas-Bernardino, J.C., García-Cruz, U., Pacheco, N. 2021. Effect of solvent polarity on the Ultrasound Assisted extraction and antioxidant activity of phenolic compounds from habanero pepper leaves (Capsicum chinense) and its identification by UPLC-PDA-ESI-MS/MS. Ultrasonics Sonochemistry, 76, 1–12. doi:10.1016/j.ultsonch.2021.105658

Huang, D.D., Shi, G., Jiang, Y., Yao, C., Zhu, C. 2020. A review on the potential of Resveratrol in prevention and therapy of diabetes and diabetic complications. Biomedicine & Pharmacotherapy, 125, 109767. doi: 10.1016/j.biopha.2019.109767

Hussain, Z., Khan, J.A., Arshad, M.I., Muhammad, F., Abbas, R.Z. 2021. Comparative characterization of cinnamon, cinnamaldehyde and kaempferol for phytochemical, antioxidant and pharmacological properties using acetaminophen-induced oxidative stress mouse model. Boletin Latinoamericano y del Caribe Plantas Medicinales y Aromaticas. 20(4):339–350. doi:10.37360/blacpma.21.20.4.25.

Jug, G., Anderluh, M., Tomašič, T. 2015. Comparative evaluation of several docking tools for docking small molecule ligands to DC-SIGN. Journal of Molecular Modeling. 21, 1-12 DOI: 10.1007/s00894-015-2713-2

Kaabi, Y.A. 2022. Potential Roles of Anti-Inflammatory Plant-Derived Bioactive Compounds Targeting Inflammation in Microvascular Complications of Diabetes. Molecules. 27(21):7352. doi:10.3390/molecules27217352.

Kahlon, A.K., Roy, S., Sharma, A. 2010. Molecular docking studies to map the binding site of squalene synthase inhibitors on dehydrosqualene synthase of Staphylococcus aureus. Journal of Biomolecular Structure and Dynamics. 28(2), 201-210. K DOI: 10.1080/07391102.2010.10507353

Kuri-García, A., Godínez-Santillán, R. I., Mejía, C., Ferriz-Martínez, R. A., García-Solís, P., Enríquez-Vázquez, A., García-Gasca T., Guzmán-Maldonado S.H., Chávez-Servín, J. L. 2019. Preventive effect of an infusion of the aqueous extract of Chaya leaves (Cnidoscolus aconitifolius) in an aberrant crypt foci rat model induced by azoxymethane and dextran sulfate sodium. Journal of medicinal food, 22(8), 851-860. doi: 10.1089/jmf.2019.0031

Laskowski, R.A., Swindells, M.B. 2011. LigPlot+: multiple ligand-protein interaction diagrams for drug discovery. Journal of Chemical Information and Modeling. 51, 2778-2786. DOI: 10.1021/ci200227u

Li, H., Han, J., Zhao, H., Liu, X., Ma, L., Sun, C., Yin, H., Shi, Y. 2018. Investigation of the Intermolecular Hydrogen Bonding Effects on the Intramolecular Charge Transfer Process of Coumarin 340 in Tetrahydrofuran Solvent. Journal of Cluster Science. 29(4):585–592. doi:10.1007/s10876-018-1371-9.

Liu, X., Ji, D., Cui, X., Zhang, Z., Li, B., Xu, Y., Chen, T., Tian, S. 2020. p-Coumaric acid induces antioxidant capacity and defense responses of sweet cherry fruit to fungal pathogens. Postharvest Biology and Technology. 169:111297. doi:10.1016/j.postharvbio.2020.111297.

Magala, P., Klevit, R.E., Thomas, W.E., Sokurenko, E.V., Stenkamp, R.E. 2020. RMSD analysis of structures of the bacterial protein FimH identifies five conformations of its lectin domain. Proteins 88(4), 593-603.M DOI: 10.1002/prot.25840

Ouadi, Y.E., Bendaif, H., Mrabti, H.N., Elmsellem, H., Kadmi, Y., Shariati, M.A., Abdel-Rahman, I., Hammouti, B., Bouyanzer, A. 2017. Antioxidant activity of phenols and flavonoids contents of aqueous extract of Pelargonium graveolens orgin in the North-East Morocco. Journal of Microbiology Biotechnology and Food Sciences. 6(5):1218–1220. doi:10.15414/jmbfs.2017.6.5.1218-1220.

Pettersen, E.F., Goddard, T.D., Huang, C.C., Couch, G.S., Greenblatt, D.M., Meng, E.C., Ferrin, T.E. 2004. UCSF Chimera--a visual-ization system for exploratory research and analysis. Journal of Computational Chemistry. 25(13):1605-12. DOI: 10.1002/jcc.20084

Pérez-González, M.Z., Macías-Rubalcava, M.L., Hernández-Ortega, S., Siordia-Reyes, A.G., Jiménez-Arellanes, M.A. 2019. Additional compounds and the therapeutic potential of Cnidoscolus chayamansa (McVaugh) against hepatotoxicity induced by antitubercular drugs. Biomedicine & Pharmacotherapy 117:1–10. doi:10.1016/j.biopha.2019.109140.

Pisoschi, A.M., Pop, A., Iordache, F., Stanca, L., Predoi, G., Serban, A.I. 2021. Oxidative stress mitigation by antioxidants-an overview on their chemistry and influences on health status. European Journal of Medicinal Chemistry, 209, 112891. doi: 10.1016/j.ejmech.2020.112891.

Reuter, S., Gupta, S.C., Chaturvedi, M.M., Aggarwal, B.B. 2010. Oxidative stress, inflammation, and cancer: How are they linked? Free Radical Biology and Medicine. 49(11):1603–1616. doi:10.1016/j.freeradbiomed.2010.09.006.

Rodrigues, M.M.R., Ojeda, J.C.M., Díaz, M.G., Allende DKB. 2021. Use of chaya (Cnidoscolous chayamansa) leaves for nutritional compounds production for human consumption. The Journal of the Mexican Chemical Society. 65:118–128. doi:10.29356/jmcs.v65i1.1433.

Ruiz, J.C.R., Ordoñez, Y.B.M., Basto, Á.M., Campos, M.R.S. 2015. Antioxidant capacity of leaf extracts from two Stevia rebaudiana Bertoni varieties adapted to cultivation in Mexico. Nutrición Hospitalaria. 31(3):1163–1170. doi:10.3305/nh.2015.31.3.8043.

Saiga, A., Tanabe, S., Nishimura, T. 2003. Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment. Journal of Agricultural and Food Chemistry. 51(12):3661–3667. doi:10.1021/jf021156g

Sissi, C., Vazquez, E., Chemello, A., Mitchenall, L.A., Maxwell, A., Palumbo, M. 2010. Mapping simocyclinone D8 interaction with DNA gyrase: evidence for a new binding site on GyrB. Antimicrobial Agents and Chemotherapy. 54(1), 213-22S DOI: 10.1128/AAC.00972-09

Sudha, G., Priya, M.S., Shree, R.I., Vadivukkarasi, S. 2011. In vitro free radical scavenging activity of raw pepino fruit (Solanum muricatum aiton). International Journal of Current Pharmaceutical Research. 3(2):137–140.

Tang, S., Chen, S., Tan, X., Xu, M., & Xu, X. (2022). Network pharmacology prediction and molecular docking-based strategy to explore the pharmacodynamic substances and mechanism of “Mung Bean” against bacterial infection. Drug Development and Industrial Pharmacy, 48(2), 58-68.

Us-Medina, U., Millán-Linares, M.D.C., Arana-Argaes E., Segura-Campos, M.R. 2020. In vitro antioxidant and anti-inflammatory activity of Chaya extracts (Cnidoscolus aconitifolius (Mill.) IM Johnst). Nutricion Hospitalaria, 37(1), 46-55.

Vazquez-Armenta, F.J., Leyva, J.M., Mata-Haro, V., Gonzalez-Aguilar, G.A, Cruz-Valenzuela, M.R., Esqueda, M., Gutierrez, A., Nazzaro, F., Fratianni, F., Gaitan-Hernandez, R., Ayala-Zavala, J.F. 2022. Phenolic compounds of Phellinus spp. with antibacterial and antiviral activities. Brazilian Journal of Microbiology,53(3), 1187-1197. doi:10.1007/s42770-022-00745-x

Waterhouse, A., Bertoni, M., Bienert, S., Studer, G., Tauriello, G., Gumienny, R., Heer, F.T., de Beer, T.A.P., Rempfer, C., Bordoli, L., Lepore, R., Schwede, T. SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Research. 46, W296-W303 (2018)

Wu, S.C., Liu, F., Zhu, K., Shen, J. Z. 2019. Natural products that target virulence factors in antibiotic-resistant Staphylococcus aureus. Journal of Agricultural and Food Chemistry, 67(48), 13195-13211.

Publicado

2024-05-13

Cómo citar

Posada-Mayorga, K. Y., Ruiz-Ruiz, J. C., Olivo-Vidal, Z. E., Lobato-Tapia, C. A., Pacheco-López, N. A., Herrera-Pool, I. E., … Sánchez Chino, X. M. (2024). Actividad antioxidante y antimicrobiana in vitro e in silico de extractos etanólicos de hojas de Cnidoscolus chayamansa. Biotecnia, 26, e2233. https://doi.org/10.18633/biotecnia.v26.2233

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