Actividad antioxidante y antimicrobiana in vitro e in silico de extractos etanólicos de hojas de Cnidoscolus chayamansa

Autores/as

  • Karla Y. Posada-Mayorga Departamento de Salud, El Colegio de la Frontera Sur-Villahermosa
  • Jorge C. Ruíz-Ruíz Escuela de Nutrición, Universidad Anáhuac Mayab
  • Zendy Evelyn Olivo Vidal Departamento de Salud, El Colegio de la Frontera Sur-Villahermosa
  • Carlos A. Lobato-Tapia Universidad Politécnica Metropolitana de Puebla
  • Neith A. Pacheco-López Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C.
  • Iván E. Herrera-Pool Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C.
  • Cesar A. Irecta Najera Departamento de Salud, El Colegio de la Frontera Sur-Villahermosa
  • Xariss Miryam Sánchez Chino CONACYT-El Colegio de la Frontera Sur

DOI:

https://doi.org/10.18633/biotecnia.v26.2233

Palabras clave:

hojas de chaya, compuestos fenólicos, docking molecular, actividad antioxidante, actividad antibacteriana

Resumen

Las hojas de Cnidoscolus chayamansa ―utilizadas en la gastronomía y la medicina tradicional en México― son ricas en compuestos fenólicos que pueden tener actividad antioxidante y antimicrobiana. En este estudio evaluamos la actividad antioxidante in vitro y la actividad antibacteriana in silico de extractos etanólicos de hojas de C. chayamansa obtenidos por ultrasonicación. El contenido de compuestos fenólicos fue de 14.37 mg GAE ml-1. Se lograron identificar compuestos como nucleósido de guanosina, ácido cumárico y los derivados de kaempferol mediante UPLC-PDA-ESI-MS. Los extractos tuvieron actividad antioxidante por medio de la quelación del Cu2+ (65.53%) y el poder reductor del Fe3+ (69.59%). Aunque no se encontró actividad antibacteriana contra E. coli y S. aureus, por medio de la inhibición de crecimiento en disco, el análisis in silico reveló que los compuestos fenólicos aislados modifican las vías de señalización esenciales para la supervivencia de las bacterias estudiadas.

 

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Publicado

2024-05-13

Cómo citar

Posada-Mayorga, K. Y., Ruiz-Ruiz, J. C., Olivo-Vidal, Z. E., Lobato-Tapia, C. A., Pacheco-López, N. A., Herrera-Pool, I. E., … Sánchez Chino, X. M. (2024). Actividad antioxidante y antimicrobiana in vitro e in silico de extractos etanólicos de hojas de Cnidoscolus chayamansa. Biotecnia, 26, 306–314. https://doi.org/10.18633/biotecnia.v26.2233

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