Caracterización del genoma completo y análisis de microsatélites mediante un enfoque in silico en pavos domésticos (Meleagris gallopavo)
DOI:
https://doi.org/10.18633/biotecnia.v27.2455Palabras clave:
in sílico, pavos, motivos, microsatélites, Meleagris gallopavoResumen
El pavo doméstico (Meleagris gallopavo) es el segundo mayor contribuyente a la producción de carne de aves de corral, después de los pollos. El estudio de la organización y distribución de microsatélites es muy importante en los estudios genómicos y evolutivos. La minería in silico de microsatélites aprovecha el poder de la biología computacional para agilizar, mejorar el descubrimiento de marcadores de microsatélites y reducir el costo de la detección de microsatélites. El presente estudio tuvo como objetivo evaluar la minería in silico de loci de microsatélites en el genoma del pavo doméstico. Se obtuvieron secuencias de referencia de varios cromosomas de NBCI y se analizaron utilizando el software Krait. El cromosoma 4 tuvo el mayor número de microsatélites perfectos, mientras que el cromosoma 18 tuvo el menor número. Sin embargo, el cromosoma 27 tuvo la mayor abundancia relativa, seguido por el cromosoma 13. El cromosoma 18 nuevamente tuvo la menor abundancia relativa. El cromosoma 4 tuvo la mayor cantidad de microsatélites imperfectos y el cromosoma 18 tuvo la menor cantidad. Se diseñaron un total de 121.248 cebadores de microsatélites. Estos loci y marcadores microsatélites desempeñarán un papel importante en el mapeo de ligamiento y mejorarán significativamente la investigación sobre la genética del pavo.
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