Simbiosis Crotalaria-Rhizobium como remediadoras de la contaminación por petróleo crudo

Ana Guadalupe  Ramírez-May; María del Carmen Rivera-Cruz; Consuelo del Carmen Bautista-Muñoz; Rocío Guadalupe Acosta-Pech; María Remedios Mendoza-López; Antonio Trujillo-Narcía

doi:10.18633/biotecnia.v27.2478

Authors

Ana Guadalupe Ramírez-May Manejo y Conservación de los Recursos Naturales, Doctorado en Ciencias Agrícolas en el Trópico, Colegio de Postgraduados Campus Tabasco, Km 3.5 Periférico Carlos A. Molina, CP 86500 H. Cár-denas, Tabasco, México https://orcid.org/0000-0003-1939-6765
María del Carmen Rivera-Cruz Manejo y Conservación de los Recursos Naturales, Doctorado en Ciencias Agrícolas en el Trópico, Colegio de Postgraduados Campus Tabasco, Km 3.5 Periférico Carlos A. Molina, CP 86500 H. Cár-denas, Tabasco, México https://orcid.org/0000-0003-4178-7252
Consuelo del Carmen Bautista-Muñoz Manejo y Conservación de los Recursos Naturales, Doctorado en Ciencias Agrícolas en el Trópico, Colegio de Postgraduados Campus Tabasco, Km 3.5 Periférico Carlos A. Molina, CP 86500 H. Cár-denas, Tabasco, México https://orcid.org/0000-0002-5207-6547
Rocío Guadalupe Acosta-Pech Manejo y Conservación de los Recursos Naturales, Doctorado en Ciencias Agrícolas en el Trópico, Colegio de Postgraduados Campus Tabasco, Km 3.5 Periférico Carlos A. Molina, CP 86500 H. Cár-denas, Tabasco, México https://orcid.org/0000-0002-8855-765X
María Remedios Mendoza-López Instituto de Química Aplicada, Universidad Veracruzana. CP 91190. Xalapa, Veracruz. México
Antonio Trujillo-Narcía Cuerpo académico: Energía y Medio Ambiente. Universidad Popular de la Chontalpa, Carretera Cárdenas-Huimanguillo km 2, Ra. Paso y Playa, CP 86500 H. Cárdenas, Tabasco, México

DOI:

https://doi.org/10.18633/biotecnia.v27.2478

Keywords:

Legumes, Total petroleum hydrocarbons, Nodules, Rhizosphere

Abstract

The objective of this research was to identify variables of
growth, production and microbial symbiotic activity in
Crotalaria incana and Crotalaria pallida that, in response to
the stress caused by crude petroleum oil, manage to remove total petroleum hydrocarbons (TPH) from the soil and can be recommended as basic tools in biological technologies for the recovery of degraded soils in the Mexican humid tropics. Crotalaria species were subjected to induced crude petroleum oil contamination at doses of 3, 15, 30, and 45 g/kg in soil and a control treatment at 30 and 154 d of exposure. The results showed statistically significant differences (Duncan p ≤ 0.05) in the growth of the two legumes, with both species being tolerant. The increase in petroleum doses caused negative effects on dry matter production of C. incana and C. pallida. Colonization by Rhizobium spp. was stimulated by crude oil in soil and rhizosphere in both species. However, at day 154, the highest rates of TPH removal were induced at doses of 30 and 45 g/kg, respectively.

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