Innovador método de electrohilado/ baño de coagulación para obtener nanofibras entrecruzadas basadas en alginato de calcio y alcohol polivinilo y su evaluación citotóxica in vitro

Tonantzi Pérez Moreno; M. J. Rivas-Arreola; I. Santos-Sauceda; R. Ramirez-Bon Ramirez-Bon; E.A. Elizalde-Peña

doi:10.18633/biotecnia.v27.2641

Authors

Tonantzi Pérez Moreno Facultad de Ingeniería, Universidad Autónoma de Querétaro, Carr. A Chichimequillas S/N, Ejido Bolaños, 76140 Santiago de Querétaro, Qro. México. https://orcid.org/0000-0001-9699-684X
M. J. Rivas-Arreola Departamento de Ciencias e Ingenierías, Universidad Iberoamericana Puebla, Blvd. Del Niño Poblano 2901, Reserva Territorial Atlixcáyotl, Centro Comercial Puebla, 72810 San Andrés Cholula, Pue. México. https://orcid.org/0000-0001-8874-3818
I. Santos-Sauceda Departamento de Investigación en Polímeros y Materiales, Universidad de Sonora, Blvd. Luis Encinas J, Calle Av. Rosales &, Centro, 83000 Hermosillo, Son. https://orcid.org/0009-0008-0675-0765
R. Ramirez-Bon Centro de Investigación y de Estudios Avanzados del IPN, Unidad Querétaro, Apdo, Postal 1-798, Querétaro, Qro, México. https://orcid.org/0000-0001-8939-6731
E.A. Elizalde-Peña Facultad de Ingeniería, Universidad Autónoma de Querétaro, Carr. A Chichimequillas S/N, Ejido Bolaños, 76140 Santiago de Querétaro, Qro. México

DOI:

https://doi.org/10.18633/biotecnia.v27.2641

Keywords:

Tissue engineering, skin wound , PVA/alginate nanofibers, coaxial electrospinning

Abstract

In tissue engineering, the availability of a scaffold is crucial; it must support cell growth, be biocompatible, and enable adequate perfusion of aqueous media. Although alginate is an approved polymer for biomedical applications, its processing to obtain membranes through electrospinning presents significant challenges. This work focuses on an efficient method to produce crosslinked nanofibers of alginate (NaAlg) and polyvinyl alcohol (PVA) using a coagulation bath with CaCl2 during electrospinning, employing both single (SN) and coaxial (CN) needles. In the case of SN, the molar ratio was evaluated for the influence on fiber formation via electrospinning; meanwhile, for CN, the tip-to-collector distance was evaluated. Obtaining optimal conditions at 70 % NaAlg for SN, and 15 cm tip-to-collector distance for CN, both at a flow rate of 0.1 mL/h and a voltage of 19 kV. The resulting crosslinked nanofibers, with average diameters of 70 ± 17 nm for SN and 124 ± 20 nm for CN, displayed well-defined interconnected fibrous morphologies. The interactions between NaAlg and CaCl2 were confirmed to be carboxylate calcium formation by FTIR. In vitro studies of CN NaAlg-PVA with JB6 fibroblast cells showed a cell proliferation rate of 124 %, demonstrating the substantial potential of these nanofibers for wound healing applications.

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