EFFECT OF THE ROOTS EXTRACT FROM Heliopsis longipes ON Aspergillus parasiticus GROWTH

Autores/as

  • John M. Velez-Haro Departamento de Ingeniería Bioquímica, Instituto Tecnológico de Celaya, Antonio García Cubas 600, Celaya, Guanajuato, México. CINVESTAV, Departamento de Ingeniería Genética, Km. 9.6 Libramiento Norte Carretera Irapuato-León, CP 36821, Irapuato, Guanajuato, México
  • Nydia E. Buitimea-Cantúa Tecnologico de Monterrey, Centro de Biotecnología-FEMSA, Escuela de Ingeniería y Ciencias. Av. Eugenio Garza Sada 2501, Monterrey, N.L., C.P. 64849, México
  • Ema C. Rosas-Burgos Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales s/n Col. Centro, CP 83000, Hermosillo, Sonora, México
  • Jorge Molina-Torres CINVESTAV, Departamento de Biotecnología y Bioquímica, Km. 9.6 Libramiento Norte Carretera Irapuato-León, CP 36821, Irapuato, Guanajuato, México
  • Génesis V. Buitimea-Cantúa Tecnologico de Monterrey, Centro de Biotecnología FEMSA, Escuela de Ingeniería y Ciencias. Av. Eugenio Garza Sada 2501, Monterrey, N.L., C.P. 64849, México CINVESTAV, Departamento de Biotecnología y Bioquímica, Km. 9.6 Libramiento Norte Carretera Irapuato-León, CP 36821, Irapuato, Guanajuato, México

DOI:

https://doi.org/10.18633/biotecnia.v20i3.720

Palabras clave:

Aflatoxins production, Alkamide content, Antifungal effect, Aspergillus parasiticus, Heliopsis longipes roots

Resumen

The organic extracts from Heliopsis longipes roots possess interesting biological and pharmacological activities. However, the effect on Aspergillus parasiticus has not been investigated so far. This study was aimed to evaluate the antifungal effect of the ethanolic extract from H. longipes roots against A. parasiticus growth. Four extract concentrations, 50, 75, 150 and 200 μg/mL were evaluated for antifungal effect, and the anti-aflatoxigenic assay was tested at 150 and 200 μg/mL. The minimal inhibitory concentration that inhibited 50 and 99% of growth (MIC50 and MIC99) were determined. Ethanolic extract was characterized by GC-EIMS analysis and its main bioactive compounds were identified. All tested concentrations inhibit the radial growth of A. parasiticus and the MIC50 and MIC99 were 116.94 and 1593.98 μg/mL, respectively. These results showed the first evidence of the antifungal effect of H. longipes on the radial growth and spore germination rate of this pathogenic fungus. The antifungal activity of H. longipes extract was attributed to affinin (7.24 ± 0.13 mg/g of fresh tissue), the most abundant alkamide detected by GC-EIMS analysis. Although, the extract did not inhibit the aflatoxins´ production, it can be used to prevent A. parasiticus growth before the mycotoxins production occur. Therefore, the extract has potential as natural antifungal agent against A. parasiticus contamination.

Descargas

Los datos de descargas todavía no están disponibles.

Citas

Buitimea-Cantúa, G.V., Rosas-Burgos, E.M., Cinco-Moroyoqui, F.J., Burgos-Hernández, A., Plascencia-Jatomea, M., Cortez-Rocha, M.O., Gálvez-Ruiz, J.C. 2013. In vitro effect of antifungal fractions from the plants Baccharis glutinosa and Jacquinia macrocarpa on chitin and β-1,3-glucan hydrolysis of maize phytopathogenic fungi and on the fungal β-1,3-glucanase and chitinase activities. Journal of Food Safety. 33: 526-535.

Castella, G., Bragulat, M.R., Cabañes, F.J. 1999. Fumonisin production by Fusarium species isolated from cereals and feeds in Spain. Journal of food protection. 62: 811-813.

Cota-Arriola, O., Cortez-Rocha, M.O., Rosas-Burgos, E.C., Burgos Hernández, A., López-Franco, Y.L., Plascencia-Jatomea, M. 2011. Antifungal effect of chitosan on the growth of Aspergillus parasiticus and production of aflatoxin B1. Polymer International. 60: 937-944.

Cox, P.W., Paul, G.C., Thomas, C.R. 1998. Image analysis of the morphology of filamentous micro-organisms. Microbiology. 144: 817-827.

Diener, U.L., Cole, R.J., Sanders, T.H., Payne, G.A., Lee, L.S., Klich, M.A. 1987. Epidemiology of aflatoxin formation by Aspergillus flavus. Annual Review of Phytopathology. 25: 249-270.

Dorner, J.W., Cole, R.J., Diener, U.L. 1984. The relationship of Aspergillus flavus and Aspergillus parasiticus with reference to production of aflatoxins and cyclopiazonic acid. Mycopathology. 87: 13-15.

El-Ghaouth, A. 1997. Biologically based alternatives to synthetic fungicides for the control of postharvest diseases. Journal of Industrial Microbiology and Biotechnology. 19: 160-162.

Fandohan, P., Gbenol, J.D., Gnonlonfin, B., Hell, K., Marasas, W.F.O., Wingfield, M.J. 2004. Effect of essential oils on the growth of Fusarium verticillioides and fumonisina contamination in corn. Journal of agricultural and food chemistry. 52: 6824-6829.

Fisher, T.R. 1957. Taxonomy of the genus Heliopsis (Compositae). Ohio Journal of Science. 57: 171-191.

Kim, J.H., Campbell, B.C., Mahoney, N., Chan, K.L., Molyneux, R.J. 2011. Chemosensitization of Aflatoxigenic Fungi to Antimycin A and Strobilurin Using Salicylaldehyde, a Volatile Natural Compound Targeting Cellular Antioxidation System. Mycopathologia. 171: 291-298.

Langfelder, K., B. Jahn, H. Gehringer, A. Schmidt, G. Wanner, A.A. Brakhage. 1998. Identification of a polyketide synthase gene (pksP) of Aspergillus fumigatus involved in conidial pigment biosynthesis and virulence. Medical microbiology and immunology. 187: 79-89.

Larralde, C.C., López, L.F., Viniegra, G.G. 1997. Morphometry evaluation of the specific growth rate of Aspergillus niger grown on agar plates at high glucose levels. Biotechnology and Bioengineering. 56: 287-294.

López, A.G., Theumer, M.G., Zygadlo, J.A., Rubinstein, H.R. 2004. Aromatic plant essential oils activity on Fusarium verticillioides fumonisina B1 production in corn grain. Mycopathologia. 158: 343-349.

Mahlo, S. M., Chauke, H. R., McGaw, L., Eloff, J. 2016. Antioxidant and antifungal activity of selected medicinal plant extracts against phytopathogenic fungi. African Journal of Traditional, Complementary and Alternative Medicines. 13: 216-222.

Molina-Torres, J., Salgado-Garciglia, R., Ramírez-Chávez, E., Del Rio, R.E. 1996. Purely olefinic alkamides in Heliopsis longipes and Acmella (Spilanthes) oppositifolia. Biochemical Systematics and Ecology. 24: 43-47.

Molina-Torres. J., García-Chávez, A., Ramírez-Chávez, E. 1999. Antimicrobial properties of alkamides present in flavouring plants traditionally used in Mesoamerica: affinin and capsaicin. Journal of Ethnopharmacology. 64: 241-248.

Molina-Torres, J., Salazar-Cabrera, C.J., Armenta-Salinas, C., Ramírez-Chávez, E. 2004. Fungistatic and Bacteriostatic activity of alkamides from Heliopsis longipes roots: affinin and reduced amides. Journal of agricultural and food chemistry. 52: 4700-4704.

Nelson, P.E. 1992. Taxonomy and biology of Fusarium moniliforme. Mycopathology. 117: 29-36.

Nychas, G.J. 1995. Natural antimicrobials from plants. In: Gould GW, editor. New methods of food preservation. London: Blackie Academic, 58-89.

Paul, G.C., Kent, C.A., Thomas, C.R. 1993. Viability testing and characterization of germination of fungal spores by automatic image analysis. Biotechnology and Bioengineering. 42: 11-23.

Plascencia-Jatomea, M.G., Viniegra, R., Olayo, M.M., Castillo- Ortega, K.S. 2003. Effect of chitosan and temperature on spore germination of Aspergillus niger. Macromolecular Bioscience. 3: 582-586.

Rai, M.K., Varma, A., Pandey, A.K. 2004. Antifungal potential of Spilanthes calva after inoculation of Piriformospora indica. Mycoses. 47: 479-481.

Rajani, P., Sridevi, V., Lakshmi, C. M. V. V., Kumari, K. S. P. 2012. Inhibitory effect of aqueous plant extracts on the growth of aflatoxin producing Aspergillus parasiticus (NCIM 898). International Journal of Engineering Science and Advanced Technology. 2: 365-371.

Ramírez-Chávez, E., Lucas-Valdez, L., Virgen-Calleros, G., Molina-Torres, J. 2000. Actividad fungicida de afinina y extractocrudo de raíces de Heliopsis longipes sobre dos especies de Sclerotium. AgroCiencia. 34: 207-215.

Rosas-Burgos, E.C., Cortez-Rocha, M.O., Cinco-Moroyoqui, F.C., Robles-Zepeda, R.E., López-Cervantes, J., Sánchez-Machado, D.I., Lares-Villa, F. 2009. Antifungal activity in vitro of Baccharis mglutinosa and Ambrosia confertiflora extracts on Aspergillus flavus, Aspergillus parasiticus, and Fusarium verticillioides. World Journal of Microbiology and Biotechnology. 25: 2257-2261.

Rosas-Burgos, E.C., Cortez-Rocha, M.O., Plascencia-Jatomea, M., Cinco-Moroyoqui, F.C., Robles-Zepeda, R.E., López-Cervantes, J., Sánchez Machado, D.I., Lares-Villa, F. 2011. The effect of Baccharis glutinosa extract on the growth of mycotoxigenic fungi and fumonisin B1 and aflatoxin B1 production. World Journal of Microbiology and Biotechnology. 27: 1025-1033.

Sánchez, E., Heredia, N., García, S. 2005. Inhibition of growth and mycotoxin production of Aspergillus flavus and Aspergillus parasiticus by extracts of Agave species. International Journal of Food Microbiology. 98: 271-279.

Shephard, G.S. 2008. Determination of mycotoxins in human foods. Chemical Society Reviews. 37: 2468-2477.

Tsai, H.F., Washburn, R.G., Chang, Y.C. and Kwon-Chung, K.J. 1997. Aspergillus fumigatus arp1 modulates conidial pigmentation and complement deposition. Molecular Microbiology. 26: 175-183.

Tsai, H.F., Wheeler, M.H., Chang, Y.C., Kwon-Chung, K.J. 1999. A developmentally regulated gene cluster involved in conidial pigment biosynthesis in Aspergillus fumigatus. Journal of Bacteriology. 181: 6469-6477.

Vargas-Arispuro, I., Reyes-Báez, R., Rivera-Castañeda, G., Martínez-Téllez, M.A., Rivero-Espejel, I. 2005. Antifungal lignans from the creosotebush (Larrea tridentata). Ind Crops Prod 22, 101-107.

Vicam, L.P. 1999. Aflatest instruction manual. Watertown, MA,

USA. Wedge, D.E., Smith, B.J. 2006. Discovery and evaluation of natural

product-based fungicides for disease control of small fruits. In: Inderjit, S., Mukerji, K.G. (eds) Allelochemicals: biological control of plant pathogens and diseases. Dordrecht, Holland 1-14.

Descargas

Publicado

2018-09-07

Cómo citar

Velez-Haro, J. M., Buitimea-Cantúa, N. E., Rosas-Burgos, E. C., Molina-Torres, J., & Buitimea-Cantúa, G. V. (2018). EFFECT OF THE ROOTS EXTRACT FROM Heliopsis longipes ON Aspergillus parasiticus GROWTH. Biotecnia, 20(3), 127–134. https://doi.org/10.18633/biotecnia.v20i3.720

Número

Sección

Artículos originales

Métrica

Artículos más leídos del mismo autor/a

Artículos similares

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 > >> 

También puede Iniciar una búsqueda de similitud avanzada para este artículo.