Identificación del perfil fitoquímico y efecto del estrés lumínico sobre la capacidad antioxidante del germinado de brócoli en un dispositivo germinador rotatorio tipo tambor
DOI:
https://doi.org/10.18633/biotecnia.v21i3.1013Palabras clave:
Germinados, brócoli, estrés lumínico, capacidad antioxidante, germinador rotatorioResumen
La luz es uno de los factores ambientales más importantes que regulan el desarrollo de las plantas. El objetivo principal de este estudio fue determinar la influencia de diferentes condiciones de luz sobre la actividad antioxidante y el contenido de compuestos fenólicos en germinados de brócoli. Los germinados de brócoli fueron producidos en un germinador rotatorio con 10 s de aspersión de agua cada 90 min, 1 h de ventilación cada 3 h, 2 rpm, 99 % HR y 3 condiciones de fotoperiodo: cada uno consistió en 11 h de luz blanca, además de 5 h luz roja, 5 h de luz azul o 5 h de luz verde, emitidos por focos Leds, dependiendo del tratamiento, y 8 h de oscuridad. La condición de luz roja obtuvo 0.71 g GS/g semilla diferencia significativa (p < 0.05). La luz azul obtuvo diferencia significativa (p < 0.05) en la inhibición de ambos radicales ABTS y DPPH con una EC50 de 1.1232 mg/mL y 1.1577 mg/mL respectivamente. El mayor contenido de fenoles se obtuvo con la condición de luz roja de 10.17 mg EAG/g y el contenido de flavonoides 1.86 mg EQ/g. Las diferentes condiciones de luz favorecen el incremento de compuestos bioactivos en germinados de brócoli en el dispositivo germinador rotatorio.Descargas
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Bedascarrasbure, E., Maldonado, L., Álvarez, A. y Rodríguez, E. 2004. Contenido de fenoles y flavonoides del propoleos Argentino. Acta Farmacéutica Bonaerense. 23: 369-372.
Bian, Z.H., Yang, Q.C. y Liu, W.K. 2015. Effects of light quality on the accumulation of phytochemicals in vegetables produced in controlled environments: a review. Journal of the Science of Food and Agriculture. 95: 869-877.
Bjorkman, M., Klingen, I., Birch, A.N., Bones, A.M., Bruce, T.J., Johansen, T.J., Meadow, R., Molmann, J., Seljasen, R., Smart, L.E. y Stewart, D. 2011. Phytochemicals of Brassicaceae in plant protection and human health--influences of climate, environment and agronomic practice. Phytochemistry. 72: 538-556.
Carvalho, S.D. y Folta, K.M. 2014. Sequential light programs shape kale (Brassica napus) sprout appearance and alter metabolic and nutrient content. Horticulture Research. 1: 8.
Chaudhary, A., Choudhary, S., Sharma, U., Vig, A. y Arora, S. 2016. In vitro Evaluation of Brassica sprouts for its Antioxidant and Antiproliferative Potential. Indian Journal of Pharmaceutical Sciences. 78: 615-623.
Chauhan, E.S., Tiwari, A. y Singh, A. 2016. Phytochemical screening of red cabbage (Brassica oleracea) powder and juice-A comparative study. Journal of Medicinal Plants Studies. 4: 196-199.
Chen, Y., Zhu, Z., Guo, Q., Zhang, L. y Zhang, X. 2012. Variation in concentrations of major bioactive compounds in Prunella vulgaris L. related to plant parts and phenological stages biology research. 45: 171-175.
Cho, J.-Y., Son, D.-M., Kim, J.-M., Seo, B.-S., Yang, S.-Y., Bae, J.-H. y Heo, B.-G. 2008. Effect of LED as light quality on the germination, growth and physiological activities of broccoli sprouts. Journal of Bio-Environment Control. 17: 117-123.
Craver, K.J., Gerovac, J., Lopez, R. y Kopsell, D. 2017. Light Intensity and Light Quality from Sole-source Light-emitting Diodes Impact Phytochemical Concentrations within Brassica Microgreens. Journal of the American Society for Horticultural Science. 142: 3-12.
D’souza, C., Yuk, H.-G., Khoo, G.H. y Zhou, W. 2015. Application of Light-Emitting Diodes in Food Production, Postharvest Preservation, and Microbiological Food Safety. Comprehensive Reviews in Food Science and Food Safety. 14: 719-740.
Darko, E., Heydarizadeh, P., Schoefs, B. y Sabzalian, M.R. 2014. Photosynthesis under artificial light: the shift in primary and secondary metabolism. Philosophical Transaction of the Royal Socyety B. 369: 20130243.
Dhiman, M.I., Prashar, M.Y., Kalia, M.K. y Gill, N.S. 2015. Therapeutic And Nutritional Value Of Brassica Oleracea L. Var. Italica (Broccoli): A Review. International Journal Of Universal Pharmacy And Bio Sciences. 4: 22-23.
Fahey, J.W., Zhang, Y. y Talalay, P. 1997. Broccoli sprouts: an exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. Proceedings of the National Academy of Sciences of the United States of America. 94: 10367-10372.
Fu, T., Stewart, D., Reineke, K., Ulaszek, J., Schelsser, J. y Tortorelo, M. 2001. Use of spent irrigation water for microbiological analysis of alfalfa sprouts. Journal of Food Protection. 64: 802-806.
Fu, W., Li, P. y Wu, Y. 2012. Effects of different light intensities on chlorophyll fluorescence characteristics and yield in lettuce. Scientia Horticulturae. 135: 45-51.
Galindo, W.F., Rosales, M., Murgueitio, E. y Larrahondo, J. 1989. Sustancias antinutricionales en las hojas de Guamo, Nacedero y Matarratón. Livestock Research for Rural Development. 1: 36-47.
Gan, R.-Y., Chan, C.-L., Yang, Q.-Q., Li, H.-B., Zhang, D., Ge, Y.-Y., Gunaratne, A., Ge, J. y Corke, H. 2018. 9 - Bioactive compounds and beneficial functions of sprouted grains. In: Feng, H., Nemzer, B. y Devries, J.W. (eds.) Sprouted Grains. AACC International Press. 191-246.
García-Peña, C.M., Kim Bich, N., Bich Thu, N., Tillan Capo, J., Romero Díaz, J.A., López, O.D. y Fuste Moreno, V. 2009. Metabolitos secundarios en los extractos secos de Passiflora incarnata L., Matricaria recutita L. y Morinda citrifolia L. Revista Cubana de Plantas Medicinales. 14: 0-0.
Ghawi, S.K., Methven, L. y Niranjan, K. 2013. The potential to intensify sulforaphane formation in cooked broccoli (Brassica oleracea var. italica) using mustard seeds (Sinapis alba). Food Chemestry. 138: 1734-1741.
Gu, Y., Guo, Q., Zhang, L., Chen, Z., Han, Y. y Gu, Z. 2012. Physiological and biochemical metabolism of germinating broccoli seeds and sprouts. Journal of Agricultural of Food Chemestry. 60: 209-213.
Guija-Poma, E., Inocente-Camones M.A., Ponce-Pardo, J. y Zarzosa-Norabuena. 2015. Evaluación de la técnica 2,2-Difenil-l-Picrilhidrazilo (DPPH) para determinar la capacidad antioxidante. Horizonte Médico. 15: 56-60.
Guriya, R., Moon, A. y Talreja, K. 2015. Phytochemical profiling and characterization of bioactive compounds from Brassica oleracea. International Journal of Pharmacognosy and Phytochemical Research. 7: 825-831.
Hinojosa-Dávalos, J., Gutiérrez-Lomelí, M., Siller-López, F., Rodríguez-Sahagún, A., Morales-Del- Río, J.A., Guerrero- Medina, P.J., Del-Toro-Sánchez, C.L. (2013). Phytochemycal screening and antiinflammatory capacity of leaves from Tithonia tubaeformis. Biotecnia. 15(2): 53-60.Huché-Thélier, L., Crespel, L., Gourrierec, J.L., Morel, P., Sakr, S. y Leduc, N. 2016. Light signaling and plant responses to blue and UV radiations—Perspectives for applications in horticulture. Environmental and Experimental Botany. 121: 22-38.
Jahangir, M., Abdel-Farid, I.B., Kim, H.K., Choi, Y.H. y Verpoorte, R. 2009. Healthy and unhealthy plants: The effect of stress on the metabolism of Brassicaceae. Environmental and Experimental Botany. 67: 23-33.
Kim, E.H., Kim, S.H., Chung, J.I., Chi, H.Y., Kim, J.A. y Chung, I.M. 2005. Analysis of phenolic compounds and isoflavones in soybean seeds (Glycine max (L.) Merill) and sprouts grown under different conditions. European Food Research and Technology. 222: 201-208.
Kopsell, D.A. y Sams, C.E. 2013. Increases in shoot tissue pigments, glucosinolates, and mineral elements in sprouting broccoli after exposure to short-duration blue light from light emitting diodes. Journal of the American Society For Horticultural Science. 138: 31-37.
Kopsell, D.A., Sams, C.E., Barickman, T.C. y Morrow, R.C. 2014. Sprouting broccoli accumulate higher concentrations of nutritionally important metabolites under narrow-band light-emitting diode lighting. Journal of the American Society for Horticultural Science. 139: 469-477.
Kusmiati y Agustini, S.N.W. 2017. Potential Lutein Extract of Broccoli (Brassica oleracea L. var. italica) as Antiradical Abts (2,2 - Azinobis Acid, 3-Ethyl Benzothiazoline-6-Sulfonic Acid). 2017. 11.
Kwack, Y., Kim, K.K., Hwang, H. y Chun, C. 2015. Growth and quality of sprouts of six vegetables cultivated under different light intensity and quality. Horticulture, Environment, and Biotechnology. 56: 437-443.
Lefsrud, M.G., Kopsell, D.A. y Sams, C.E. 2008. Irradiance from distinct wavelength light-emitting diodes affect secondary metabolites in kale. HortScience. 43: 2243-2244.
Li, Q. y Kubota, C. 2009. Effects of supplemental light quality on growth and phytochemicals of baby leaf lettuce. Environmental and Experimental Botany. 67: 59-64.
Long, J.C. y Jenkins, G.I. 1998. Involvement of plasma membrane redox activity and calcium homeostasis in the UV-B and UV-A/blue light induction of gene expression in Arabidopsis. Plant Cell. 10: 2077-2086.
Maksimović, Z., Malenčić, Đ. y Kovačević, N. 2005. Polyphenol contents and antioxidant activity of Maydis stigma extracts. Bioresource Technology. 96: 873-877.
Manchali, S., Chidambara Murthy, K.N. y Patil, B.S. 2012. Crucial facts about health benefits of popular cruciferous vegetables. Journal of Functional Foods. 4: 94-106.
Martínez- Valverde, I., Periago, M.J. y Ros, G. 2000. Significado nutricional de los compuestos fenólicos de la dieta. Archivos Latinoamericanos de Nutrición. 50: 5-18.
McCree. 1972. The action spectrum, absorptance and quantum yield of photosynthesis in crop plants. Agricultural Meteorology. 9: 191-216.
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science. 7: 405-410.
Molyneux, P. 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal Science and Technology. 26: 211-219.
Morales-Del-Rio, J.A., Gutiérrez-Lomelí, M., Robles-García, M.A., Aguilar, J.A., Lugo-Cervantes, E., Guerrero-Medina, P.J., Ruiz- Cruz, S., Cinco-Moroyoqui, F.J., Wong-Corral, F.J., Del-Toro- Sánchez, C.L. 2015. Anti-inflammatory activity and changes in antioxidant properties of leaf and stem extracts from Vitex mollis Kunth during in vitro digestion. Evidence-Based Complementary and Alternative Medicine (eCAM). 2015. Doi: http://dx.doi.org/10.1155/2015/349235.
Moreno, D.A., Carvajal, M., Lopez-Berenguer, C. y Garcia- Viguera, C. 2006. Chemical and biological characterisation of nutraceutical compounds of broccoli. Journal of Pharmceutical Biomedical Analilys. 41: 1508-1522.
Moreno, D.A., Pérez-Balibrea, S., Ferreres, F., Gil-Izquierdo, Á. y García-Viguera, C. 2010. Acylated anthocyanins in broccoli sprouts. Food Chemistry. 123: 358-363.
Olle, M. y Viršile, A. 2013. The effects of light-emitting diode lighting on greenhouse plant growth and quality. Agricultural and Food Science. 22: 223-234.
Paniagua-Pardo, G., Hernández-Aguilar, C., Rico-Martínez, F., Domínguez-Pacheco, F.A., Martínez-Ortiz, E. y Martínez- González, C.L. 2015. Efecto de la luz led de alta intensidad sobre la germinación y el crecimiento de plántulas de brócoli (Brassica oleracea L.). Polibotánica. 40: 199-212.
Pérez-Balibrea, S., Moreno, D.A. y García-Viguera, C. 2008. Influence of light on health-promoting phytochemicals of broccoli sprouts. Journal of the Science of Food and Agriculture. 88: 904-910.
Pérez-Nájera, V.C., Lugo, E., Gutiérrez-Lomelí, M. y Del Toro, L. 2013. Extracción de compuestos fenólicos de la cáscara de lima (Citrus limetta risso) y determinación de su actividad antioxidante. Biotecnia. 15: 18-22.
Perez-Perez, L.M., García-Borbón, L., González-Vega, R.I., JoséCarlos Rodríguez-Figueroa, J.C., Rosas-Burgos, E.C., Huerta- Ocampo, J.A., Ruiz-Cruz, S., Wong-Corral, F.J., Borboa-Flores, J., Rueda-Puente, E.O. y Del-Toro-Sánchez, C.L. 2018. Liberación de compuestos fenólicos ligados en el garbanzo (Cicer arietinum L.) utilizando microbiota humana intestinal. Biotecnia. 20(3), 146-154.
Prior, R.L., Wu, X. y Schaich, K. 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journals Agricultural and Food Chemestry. 53: 4290-4302.
Qian, H., Liu, T., Deng, M., Miao, H., Cai, C., Shen, W. y Wang, Q. 2016. Effects of light quality on main health-promoting compounds and antioxidant capacity of Chinese kale sprouts. Food Chemestry. 196: 1232-1238.
Ramírez-Rojo, M. I., Vargas-Sánchez, R. D., del Mar Torres- Martínez, B., Torrescano-Urrutia, G. R., y Sánchez-Escalante, A. (2018). Extractos de hojas de plantas para conservar la calidad de la carne y los productos cárnicos frescos. Revisión. Biotecnia. 20(3), 155-164.
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. y Rice- Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine. 26: 1231-1237.
Rodríguez-Hernández, M.D.C., Moreno, D.A., Carvajal, M. y Martínez-Ballesta, M.D.C. 2014. Genotype Influences Sulfur Metabolism in Broccoli (Brassica oleracea L.) Under Elevated CO2 and NaCl Stress. Plant and Cell Physiology. 55: 2047- 2059.
Sánchez-García, Y., Rondón-Arias, L., Hermosilla-Espinosa, R. y Almeida- Saavedra, M. 2010. Tamizaje fitoquímico de los extractos alcohólico, etéreo y acuoso de las hojas, tallos y flores de la Helychrysum bracteatum. Química Viva. 9: 40-45.
Sikin, A.M., Zoellner, C. y Rizvi, S.S. 2013. Current intervention strategies for the microbial safety of sprouts. Journal Food Protection. 76: 2099-2123.
Solanilla, D.J.F., Lombo, O., Perae, M.E. Y Méndez, A.J.J. 2011. Valoración del potencial antioxidante de Mollinedia racemosa (romadizo). Revista Cubana de Plantas Medicinales. 16: 151-163.Tian, X. y Schiach, K.M. 2013. Effects of molecular structure on kinetics and dynamics of the trolox equivalent antioxidant capacity assay with ABTS+•. Journals of Agricultural and Food Chemistry. 61: 5511-5519.
Tong-Jen F., Reineke, K.F., Chirtel, S. y VanPelt, O.M. 2008. Factors influencing the growth of Salmonella during sprouting of naturally contaminated alfalfa seeds. Journal of food protection. 71: 888-896.
Vale, A.P., Cidade, H., Pinto, M. y Oliveira, M.B. 2014. Effect of sprouting and light cycle on antioxidant activity of Brassica oleracea varieties. Food Chemestry. 165: 379-87.
Verkerk, R., Schreiner, M., Krumbein, A., Ciska, E., Holst, B., Rowland, I., De Schrijver, R., Hansen, M., Gerhauser, C., Mithen, R. y Dekker, M. 2009. Glucosinolates in Brassica vegetables: the influence of the food supply chain on intake, bioavailability and human health. Molecular Nutrition Food Research. 53: S219-S265.
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