Estudio comparativo de la producción de biomasa y omega 3 y 6 en Thraustochytrium kinney
DOI:
https://doi.org/10.18633/biotecnia.v23i2.1405Palabras clave:
Ácidos grasos poliinsaturados, glicerol crudo, aceites microbianos, ácido docosahexaenoico (DHA).Resumen
Thraustochytridos se caracterizan por producir aceites microbianos del tipo omega 3 y 6. VAL-B1 y EMA-T5 son cepas del mismo género y aprovechan fuentes puras de carbono y residual para producir ácidos grasos poliinsaturados. Se utilizó glicerol puro (GP) y crudo (GC) para comparar la producción de biomasa y el rendimiento y producción de omega 3 y 6, usando 3 concentraciones diferentes de sustrato. Empleando 100 mL de cultivo a 25°C y 180 rpm, se crecieron las cepas por 7 días, luego se centrifugaron y cuantifico la biomasa y por cromatografía gaseosa el perfil de ácidos grasos. Los resultados muestran que EMA-T5 genera mayor cantidad de biomasa al 5 día usando 20 g/L de GC con 6,984±0,66 g/L. Respecto al rendimiento y producción de omega 3 y 6, VAL-B1 obtuvo los mejores resultados con 91,80±8,78 mg/g y 1167,71±70,88 mg/L, siendo el omega 3 más abundante, en especial DHA con 52,72±4,94 % de ácidos grasos totales (AGT). Se concluye, que EMA-T5 genera mayor producción de biomasa usando GC y VAL-B1 presenta los mejores resultados de rendimiento y producción de omega empleando GP, lo cual indica que ambas cepas, aprovechan de buena forma tanto la fuente pura como cruda del glicerol.
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