Comparative study of the production of biomass and omega 3 and 6 in Thraustochytrium kinney
DOI:
https://doi.org/10.18633/biotecnia.v23i2.1405Keywords:
Polyunsaturated fatty acids, crude glycerol, microbial oils, docosahexaenoic acid.Abstract
Thraustochytrids are characterized by producing omega 3 and 6 type microbial oils. VAL-B1 and EMA-T5 are strains of the same genus and take advantage of pure sources of carbon and residual to produce polyunsaturated fatty acids. Pure (GP) and crude (GC) glycerol were used to compare biomass production and omega 3 and 6 yield and production, using 3 different substrate concentrations. Using 100 mL of culture at 25 ° C and 180 rpm, the strains were grown for 7 days, then the biomass was centrifuged and the fatty acid profile was quantified by gas chromatography. The results show that EMA-T5 generates a greater amount of biomass at day 5 using 20 g/L of GC with 6.984±0.66 g/L. Regarding the yield and production of omega 3 and 6, VAL-B1 obtained the best results with 91.80±8.78 mg/g and 1167.71±70.88 mg/L, being the most abundant omega 3, especially DHA which presented the highest abundance with 52.72±4.94 % total fatty acids (TFA). It is concluded that EMA-T5 generates higher biomass production using GC and VAL-B1 presents the best performance and omega production results using GP, which indicates that both strains make good use of both the pure and raw sources of glycerol.
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