Monitoreo de la microbiota del hepatopáncreas asociada a genes de toxinas pirABVP en Penaeus vannamei

Monitoreo de la microbiota del hepatopáncreas

Autores/as

  • Angelica Ávila-Villa Departamento de Ciencias de la Salud, Universidad de Sonora, Campus Cajeme, C.P 85010, Cd. Obre-gón, Sonora, México.
  • Gabriel Barco-Mendoza Laboratorio de Biotecnología y Trazabilidad Molecular de los Alimentos. Instituto Tecnológico de Sonora, CP.85000, Ciudad Obregón, Sonora, México
  • Roberto Rodriguez-Ramirez Instituto Tecnológico de Sonora
  • Daniel Villanueva-Zayas Laboratorio de Biotecnología y Trazabilidad Molecular de los Alimentos. Instituto Tecnológico de Sonora, CP.85000, Ciudad Obregón, Sonora, México
  • Marcel Martínez-Porchas Coordinación de Tecnología de Alimentos de Origen Animal (CTAOA), Centro de Investigación en Ali-mentación y Desarrollo, A.C. (CIAD), CP. 83304, Hermosillo, Sonora, México
  • Alma Guadalupe Villa-Lerma Laboratorio de Biotecnología y Trazabilidad Molecular de los Alimentos. Instituto Tecnológico de Sonora, CP.85000, Ciudad Obregón, Sonora, México
  • Belinda Vallejo-Córdoba Coordinación de Tecnología de Alimentos de Origen Animal (CTAOA), Centro de Investigación en Ali-mentación y Desarrollo, A.C. (CIAD), CP. 83304, Hermosillo, Sonora, México.
  • Josue Delgado-Domínguez Laboratorio de Biotecnología y Trazabilidad Molecular de los Alimentos. Instituto Tecnológico de Sonora, CP.85000, Ciudad Obregón, Sonora, México

DOI:

https://doi.org/10.18633/biotecnia.v26.2181

Palabras clave:

microbiota, AHPND, Vibrio, Secuenciación de ultima generacion, camaron

Resumen

La producción acuícola de camarón se ha expandido rápidamente en las últimas décadas, convirtiéndose en una importante actividad económica a nivel mundial. Sin embargo, algunas infecciones bacterianas y virales son un gran desafío para esta actividad. La Necrosis Hepatopancreática Aguda (AHPND) es una enfermedad bacteriana que afecta a los estanques de granjas camaroneras y se presenta durante los primeros 30 días después de la siembra en estanques de camarones, causando una mortalidad del 70-100%. La microbiota tiene un papel importante en la producción del camarón y el control de enfermedades. En el presente estudio, los genes pirA y pirB fueron amplificados por PCR para confirmar tanto organismos positivos como negativos a AHPND en hepatopáncreas de camarones. Posteriormente se secuenciaron las regiones hipervariables ribosómicas (ARNr) V3-V4 del gen 16S utilizando la plataforma MiSeq. Los resultados demostraron una microbiota diversa en el grupo negativo a AHPND, compuesto principalmente por filos Proteobacteria, Firmicutes y Actinobacteria. Además, Proteobacteria fue el filo dominante en el hepatopáncreas infectado con AHPND, mientras que Firmicutes tendió a disminuir. Vibrio fue el género más abundante, prevaleciendo sobre algunos géneros como Corynebacterium, Weissella, Lactobacillus, Photobacterium y Geobacillus. Los resultados sugieren que la enfermedad de AHPND causa disbiosis en la microbiota hepatopancreática, y el filo Firmicutes podría requerir ser restaurado en dicho escenario.

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Publicado

2023-12-11

Cómo citar

Ávila-Villa, L. A., Barco-Mendoza, G. A. ., Rodriguez-Ramirez, R. ., Villanueva-Zayas, J. D., Martínez-Porchas, M., Villa-Lerma, A. G. ., … Delgado-Domínguez, J. . (2023). Monitoreo de la microbiota del hepatopáncreas asociada a genes de toxinas pirABVP en Penaeus vannamei: Monitoreo de la microbiota del hepatopáncreas. Biotecnia, 26, e2181. https://doi.org/10.18633/biotecnia.v26.2181

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