Screening the microbiota of hepatopancreas associated with toxin genes pirABVP in Penaeus van-namei: SCREENING THE MICROBIOTA OF HEPATOPANCREAS

Luz Angelica Ávila-Villa; Gabriel Amílcar  Barco-Mendoza; Roberto   Rodriguez-Ramirez; Jesus Daniel Villanueva-Zayas; Marcel   Martínez-Porchas; Alma Guadalupe  Villa-Lerma; Belinda  Vallejo-Córdoba; Josué  Delgado-Domínguez

doi:10.18633/biotecnia.v26.2181

Authors

Angelica Ávila-Villa Departamento de Ciencias de la Salud, Universidad de Sonora, Campus Cajeme, C.P 85010, Cd. Obre-gón, Sonora, México.
Gabriel Barco-Mendoza Laboratorio de Biotecnología y Trazabilidad Molecular de los Alimentos. Instituto Tecnológico de Sonora, CP.85000, Ciudad Obregón, Sonora, México
Roberto Rodriguez-Ramirez Instituto Tecnológico de Sonora
Daniel Villanueva-Zayas Laboratorio de Biotecnología y Trazabilidad Molecular de los Alimentos. Instituto Tecnológico de Sonora, CP.85000, Ciudad Obregón, Sonora, México
Marcel Martínez-Porchas Coordinación de Tecnología de Alimentos de Origen Animal (CTAOA), Centro de Investigación en Ali-mentación y Desarrollo, A.C. (CIAD), CP. 83304, Hermosillo, Sonora, México
Alma Guadalupe Villa-Lerma Laboratorio de Biotecnología y Trazabilidad Molecular de los Alimentos. Instituto Tecnológico de Sonora, CP.85000, Ciudad Obregón, Sonora, México
Belinda Vallejo-Córdoba Coordinación de Tecnología de Alimentos de Origen Animal (CTAOA), Centro de Investigación en Ali-mentación y Desarrollo, A.C. (CIAD), CP. 83304, Hermosillo, Sonora, México.
Josue Delgado-Domínguez Laboratorio de Biotecnología y Trazabilidad Molecular de los Alimentos. Instituto Tecnológico de Sonora, CP.85000, Ciudad Obregón, Sonora, México

DOI:

https://doi.org/10.18633/biotecnia.v26.2181

Keywords:

microbiota, AHPND, Vibrio, next-generation sequencing, shrimp

Abstract

Shrimp aquaculture has rapidly expanded in the last decades, representing an important economic activity worldwide; however, bacterial, and viral infections are one of the biggest challenges in aquaculture activity. Acute Hepatopancreatic Necrosis Disease (AHPND) is a bacterial disease that affects shrimp farm ponds and occurs during the first 30 days after stocking in shrimp ponds, causing 70-100% mortalities. Microbiota has an important role in developing shrimp aquaculture and disease control. In the present study, pirA and pirB genes were amplified by PCR to confirm AHPND and non-AHPND in shrimp hepatopancreas; samples were sequenced using the MiSeq platform targeting the V3-V4 16S ribosomal (rRNA) hypervariable regions. Results demonstrated a diverse microbiota in the non-AHPND group, mainly composed of phyla Proteobacteria, Firmicutes, and Actinobacteria. Furthermore, Proteobacteria was the dominant phyla in the hepatopancreas infected with AHPND, while Firmicutes tended to decrease. Vibrio was the most abundant at the genus level, prevailing in some genera like Corynebacterium, Weissella, Lactobacillus, Photobacterium, and Geobacillus. Results suggest that AHPND causes dysbiosis in the hepatopancreatic microbiota, and the Firmicutes phylum could require to be restored under such scenario.

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Screening the microbiota of hepatopancreas associated with toxin genes pirABVP in Penaeus van-namei