FIBROLYTIC ACTIVITY OF PODAXIS PISTILLARIS FUNGUS IN SUBMERGED CULTURE

Autores/as

  • Agustín Rascón Chu Coordinación de Tecnología de Alimentos de Origen Vegetal. Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6, CP 83304. Hermosillo, Sonora, México.
  • Carmen Armida Contreras Vergara Coordinación de Tecnología de Alimentos de Origen Vegetal. Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6, CP 83304. Hermosillo, Sonora, México.
  • Ciria Guadalupe Figueroa Soto Coordinación de Ciencias de los Alimentos, Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6, CP 83304. Hermosillo, Sonora, México.
  • Tania Elisa González Soto Coordinación de Tecnología de Alimentos de Origen Vegetal. Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6, CP 83304. Hermosillo, Sonora, México.
  • Martín Esqueda Valle Coordinación de Tecnología de Alimentos de Origen Vegetal. Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6, CP 83304. Hermosillo, Sonora, México.
  • José Alfonso Sánchez Villegas Coordinación de Tecnología de Alimentos de Origen Vegetal. Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera a la Victoria Km 0.6, CP 83304. Hermosillo, Sonora, México.

DOI:

https://doi.org/10.18633/biotecnia.v21i1.874

Palabras clave:

cellulases, xylanases, laccases, thermostable enzyme, enzymatic activity

Resumen

Podaxis pistillaris is a fungus commonly found in most desert areas worldwide. The oval shaped peridium, the rigid woody stipe, plus a 10-15 μm spore size stand out among its morphological features. Even though this fungus is used for human consumption and for several traditional remedies, a lack of knowledge regarding its fibrolytic enzymatic system still prevails. This fungus was collected from the central region of the Sonoran desert (29° 07.23´ 97” LN and 110° 53.58´ 02” LW, 238 masl). In order to study its enzymatic system on common fibers, P. pistillaris was grown in a specific submerged culture in order to determine total cellulases, xylanases and laccases. The maximum cellulolytic (501.7 U·mg-1), as well as xylanolytic (157.8 U·mg-1) activities, were detected after 18 cultivation days, whereas the highest laccase specific activity (179.6 U·mg-1) was registered after 15 days at 40° C. The thermostability of total cellulases, xylanases and laccases was found within temperatures ranging from 40 to 60° C. The present study represents the first report of P. pistillaris fibrolytic activity in submerged culture.

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Publicado

2019-01-01

Cómo citar

Chu, A. R., Contreras Vergara, C. A., Figueroa Soto, C. G., González Soto, T. E., Esqueda Valle, M., & Sánchez Villegas, J. A. (2019). FIBROLYTIC ACTIVITY OF PODAXIS PISTILLARIS FUNGUS IN SUBMERGED CULTURE. Biotecnia, 21(1), 120–126. https://doi.org/10.18633/biotecnia.v21i1.874

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