Whey protein partitioning in aqueous two-phase systems
DOI:
https://doi.org/10.18633/biotecnia.v23i3.1263Keywords:
ATPE, high throughput screening, lactose separation, protein/(NH4)2SO4 system, whey proteinsAbstract
Whey is a by-product of cheese manufacturing that has proteins that can be recovered, but they are very diluted and in a mixture with lactose, which limits their use. The aqueous two-phase extraction technique (ATPE) is a procedure with potential to separate proteins. The objective was to evaluate the behavior of an aqueous protein-(NH4)2SO4 extraction system applied for the recovery of whey proteins, considering the reduction of lactose. A binodal phase diagram was constructed using a high throughput screening technique. Ammonium sulfate was dissolved at concentrations between 22 and 40 % using whey as solvent. The volume of the top phase of the systems was less than that of the bottom one, but the increase in (NH4)2SO4 caused a gradual increase at the top phase. Around 80 % of whey protein concentrated at the top phase, while over 79 % of lactose was at the bottom phase. The ATPE technique has the potential to separate proteins from whey and the best alternative is based on systems with 34 % (NH4)2SO4.
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