Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121

Antimicrobial peptide AP7121 in catheters

Authors

  • S Lissarrague Faculty of Medical Sciences, National University of the Center of the Province of Buenos Aires, Argentina https://orcid.org/0009-0004-0524-2620
  • L Schofs Laboratory of Pharmacology, Faculty of Veterinary Sciences, National University of the Center of the Province of Buenos Aires Aires, Argentina- CIVETAN- CONICET https://orcid.org/0000-0002-3955-601X
  • M Bistoletti Faculty of Medical Sciences, National University of the Center of the Province of Buenos Aires, Argentina https://orcid.org/0009-0001-2233-1201
  • L García Allende Faculty of Medical Sciences, National University of the Center of the Province of Buenos Aires, Argentina https://orcid.org/0009-0007-0980-0716
  • A Lallée Faculty of Medical Sciences, National University of the Center of the Province of Buenos Aires, Argentina https://orcid.org/0009-0008-9773-4604
  • B Baldaccini Faculty of Medical Sciences, National University of the Center of the Province of Buenos Aires, Argentina
  • S Sánchez Bruni Laboratory of Pharmacology, Faculty of Veterinary Sciences, National University of the Center of the Province of Buenos Aires Aires, Argentina- CIVETAN- CONICET https://orcid.org/0000-0002-9598-6786
  • Sparo Faculty of Medical Sciences, National University of the Center of the Province of Buenos Aires, Argentina https://orcid.org/0000-0002-0768-894X

DOI:

https://doi.org/10.18633/biotecnia.v25i2.1858

Keywords:

antimicrobial peptide AP7121, inhibition, vascular catheter, bacterial adherence

Abstract

Healthcare-associated bloodstream infections are the leading cause of morbidity and mortality in hospitalized patients. Vascular catheter-related infection is its main source. Gram-positive bacteria especially Staphylococcus spp., are the most prevalent etiological agents. The aim was to assess the inhibitory activity of the AP7121 on the Staphylococcus aureus adherence in vascular catheters. The biofilm-producer strain S. aureus ATCC 35556 (SA) was used. MIC AP7121 for SA was performed. 20 mm segments of the vascular catheter (n=3) were inoculated with 104 UFC mL-1 of SA. Three different treatment schemes (A: simultaneous, B: previous, and C: following bacterial challenge) using 1 x MIC AP7121 were tested. Control groups were included in each scheme. The MICAP7121 was 0.48 mg/L. A reduction of 2log10 representing a decrease of 99 % of viable SA cells was achieved with schemes A and B. The post-challenge treatment with AP7121 (scheme C) produced a reduction of 1log10 representing a decrease of 90 % of viable SA cells. The results observed in this work suggest a fast antimicrobial activity of AP7121 that could be beneficial to reduce bacterial adherence on medical devices such as vascular catheters

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Published

2023-05-11

How to Cite

Lissarrague, S., Schofs, L. ., Bistoletti, M., García Allende, L., Lallée, A., Baldaccini, B., … Sparo, M. (2023). Inhibition of bacterial adherence to vascular catheters by the antimicrobial peptide AP7121: Antimicrobial peptide AP7121 in catheters. Biotecnia, 25(2), 165–168. https://doi.org/10.18633/biotecnia.v25i2.1858

Issue

Vol. 25 No. 2 (2023): May - August

Section

Research Articles

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