Bougainvillea spectabilis as a mediator for the biosynthesis of silver nanoparticles, evaluation of its antimicrobial and cytotoxic effect
DOI:
https://doi.org/10.18633/biotecnia.v26.2257Keywords:
Biosynthesis, nanoparticles, antimicrobial, cytotoxicAbstract
The present study describes the biosynthesis, characterization, evaluation of the antimicrobial activity and cytotoxicity of silver nanoparticles (Ag-NPs) synthesized from the aqueous extract of the flowers and bracts of the plant Bougainvillea spectabilis. Different extract volumes and concentrations of silver nitrate were evaluated on the morphology, size, and antimicrobial activity of Ag-NPs. The formation of these nanoparticles (NPs) was monitored by ultraviolet-visible spectrophotometry (UV-Vis). Morphology and elemental analysis were performed by scanning electron microscopy coupled with X-ray energy dispersive spectrophotometry (SEM-EDS), the agglomerates of particles with dimensions ranging from 40 nm to 60 nm were observed. The presence of silver was validated with X-ray diffraction (XRD) analysis. The synthesis of Ag-NPs showed antimicrobial activity against some microorganisms isolated from diabetic foot infections, as well as control strains finding greater efficacy against Gram-negative bacteria than their Gram-positive counterparts. Ag-NPs showed cytotoxic activity in eukaryotic cells depending on their concentration, and in some cases increased cell proliferation at low concentrations. These results invite a more in-depth evaluation of the cytotoxic effect of these nanoparticles synthesized by this methodology for future application in the healthcare field.
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References
Agasti, N. y Kaushik, N.K. 2014. One Pot Synthesis of Crystalline Silver Nanoparticles. American Journal of Nanomaterials. 2(1): 4-7.
Bharathi, D., Kalaichelvan, P. T., Atmaram, V., & Anbu, S. 2016. Biogenic synthesis of silver nanoparticles from aqueous flower extract of Bougainvillea spectabilis and their antibacterial activity. J. Med. Plants, 4(5), 248-252.
Dakal, T.C., Kumar, A., Majumdar, R.S. y Yadav, V. 2016. Mechanistic Basis of Antimicrobial Actions of Silver Nanoparticles. Frontiers in Microbiology. 7: 1-17.
Durán N., S. Camila P., Durán M. and Martinez D. S. T. 2015. Silver nanoparticle protein corona and toxicity: a mini‑review. Journal of Nanobiotechnology. 13: 1-17.
Goutam, S. P., Saxena, G., Singh, V., Yadav, A. K., Bharagava, R. N., & Thapa, K. B. 2018. Green synthesis of TiO2 nanoparticles using leaf extract of Jatropha curcas L. for photocatalytic degradation of tannery wastewater. Chemical Engineering Journal, 336, 386-396.
Hernandez-Martinez, A.R., Estevez, M., Vargas, S., Quintanilla, F. y Rodríguez, R. 2011. New Dye-Sensitized Solar Cells Obtained from Extracted Bracts of Bougainvillea Glabra and Spectabilis Betalain Pigments by Different Purification Processes. International Journal of Molecular Sciences. 12(9): 5565-5576.
Huq, M. A., Ashrafudoulla, M., Rahman, M. M., Balusamy, S. R. y Akter, S. 2022. Green synthesis and potential antibacterial applications of bioactive silver nanoparti-
cles: a review. Polymers, 14(4), 742.
Jiao, Z.H., Li, M., Feng, Y.X., Shi, Ji.C., Zhang, J. y Shao, B. 2014. Hormesis Effects of Silver Nano-particles at Non-Cytotoxic Doses to Human Hepatoma Cells. Plos One. 9(7): 1-12.
Kaba, S.I. y Egorova, E.M. 2015. In vitro studies of the toxic effects of silver nanoparticles on HeLa and U937 cells. Nanotechnology, Science and Applications. 8: 19-29.
Kanmani, P. y Lim, S.T. 2013. Synthesis and structural characterization of silver nanoparticles using bacterialexopolysaccharide and its antimicrobial activity against food and multidrugresistant pathogens. Process Biochemistry. 48(7): 1099–1106.
Khan, I., Saeed, K. y Khan, I. 2017. Nanoparticles: properties, applications and toxicities. Arabian Journal of Chemistry, 12: 908-931.
Khan, M., Khan, M., Adil, S.F., Tahir, M.N., Tremel, W., Alkhathlan, H.Z., Al-Warthan, A. y Siddiqui, M.R.H. 2013. Green synthesis of silver nanoparticles mediated by Pulicaria glutinosa extract. In-ternational Journal of Nanomedicine. 8: 1507–1516.
Kumar, S.N.A., Ritesh, S.K., Sharmila, G. y Muthukumaran, C. 2017. Extraction optimization and char-acterization of water soluble red purple pigment from floral bracts. Arabian Journal of Chemistry. 10(2): S2145–S2150.
López-Mercado, J., González-Domínguez, M. I., Reynoso-Marin, F. J., Acosta, B., Smolentseva, E., & Nambo, A. 2023. Green synthesis of TiO2 for furfural production by photohydrolysis of tortilla manufacturing waste. Scientific Reports, 13(1), 15355.
Mahesh A.R., Ranganath M.K. and Harish Kumar D.R. 2013. Enrichment of Flavonoids from the Methanolic Extract of Boerhaavia Diffusa Roots by Partitioning Technique. Research Journal of Chemical Sciences. Vol. 3, pp: 43-47.
McShan, D., Ray, C.P. y Yu, H. 2014. Molecular Toxicity Mechanism of Nanosilver. Journal of Food Drug Analysis. 22(1): 116–127.
Milić, M., Leitingerb, G., Pavičića, I., Avdičevićd, M.Z., Dobrovićd, S., Goesslere, W. y Vrčeka, I.V. 2014. Cellular uptake and toxicity effects of silver nanoparticles in mammalian kidney cells. Journal of Ap-plied Toxicology. 35(6): 581-592.
Monteiro-Riviere, N.A., Samberg, M.E., Oldenburg, S.J. y Riviere, J.E 2013. Protein binding modulates the cellular uptake of silver nanoparticles into human cells: Implications for in vitro to in vivo extrap-olations? Toxicology Letters. 220(3): 286–293.
Nidhi, M., Vijay-Lakshmi, T., Kuldeep, D., Rekha, K. y Ashok, M. 2016. Does Bougainvillea spectabilis protect Swiss Albino Mice from Aflatoxin-induced Hepatotoxicity?. Advances in Animal and Vet-erinary Sciences. 4: 250-257.
Pantidos, N. y Horsfall, L.E. 2014. Biological Synthesis of Metallic Nanoparticles by Bacteria, Fungi and Plants. Journal of Nanomedicine & Nanotechnology. 5(5): 1-10.
Pareek, N., Dhaliwal, A.S. y Malik, C.P. 2012. Biogenic Synthesis of Silver Nanoparticles, Using Bou-gainvillea spectabilis Willd. Bract Extract. National Academy Science Letters. 35: 383–388.
Samberg, M.E., Oldenburg, S.J. y Monteiro-Riviere, N.A. 2010. Evaluation of Silver Nanoparticle Toxicity in Skin in Vivo and Keratinocytes in Vitro. Environmental Health Perspectives. 118(3): 407-413.
Shaik, M.R., Albalawi, G.H., Khan, S.T., Khan, M., Adil, S.F., Kuniyil, M., Al-Warthan, A., Siddiqui, M.R.H., Alkhathlan, H.Z. y Khan, M. 2016. “Miswak” Based Green Synthesis of Silver Nanoparti-cles: Evaluation and Comparison of Their Microbicidal Activities with the Chemical Synthesis. Molecules. 21(11): 1-15.
Shannahan, J.H., Podila, R., Aldossari, A.A., Emerson, H., Powell, B.A., Ke, P.C., Rao, A.M. y Brown, J.M. 2015. Formation of a Protein Corona on Silver Nanoparticles Mediates Cellular Toxicity via Scavenger Receptors. Toxicological Sciences. 143(1): 136–146.
Tamilselvan, A., Srividhya, P., Karuthapandian, S. y Mehalingam, P. 2016. Plant - Driven to Synthesis of Silver Nanoparticles using Crataeva religiosa Hook & Frost and its Utility in Detecting Antimicrobial and Antioxidant Assay. International Journal of Green and Herbal Chemistry. 5: 390-402.
Villanueva-Ibáñez, M., Yañez-Cruz, M. G., Álvarez-García, R., Hernández-Pérez, M.A. y Flo-res-González, M.A. 2015. Aqueous corn husk extract – mediated Green synthesis of AgCl and Ag nanoparticles. Materials Letters. 152: 166–169.
Vimalkumar, C. S., Hosagaudar, V. B., Suja, S. R., Vilash, V., Krishnakumar, N. M., & Latha, P. G. 2014. Comparative preliminary phytochemical analysis of ethanolic extracts of leaves of Olea dioica Roxb., infected with the rust fungus Zaghouania oleae (EJ Butler) Cummins and non-infected plants. Journal of Pharmacognosy and phytochemistry, 3(4): 69-72.
Zhang, X.F., Choi, Y.J., Han, J. W., Kim, E., Park, J. H., Gurunathan, S. y Kim, J.H. 2015. Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells. Inter-national Journal of Nanomedicine. 10: 1335–1357.
Zhang, X.F., Shen, W. y Gurunathan, S. 2016. Silver Nanoparticle-Mediated Cellular Responses in Various Cell Lines: An in Vitro Model. International Journal of Molecular Sciences. 17(10): 1-26.
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