Elementos genómicos asociados a la formación de biopelículas en Lactobacillus delbrueckii subespecie bulgaricus y Lactobacillus delbrueckii subespecie lactis
DOI:
https://doi.org/10.18633/biotecnia.v26.2423Palabras clave:
gen pili, gen srtA, gen inu, gen epsD, bioinformáticaResumen
Lactobacillus delbrueckii subsp. bulgaricus y L. delbrueckii subsp. lactis son dos subespecies biotecnológicamente importantes dentro del grupo de bacterias ácido-lácticas. Ambas se emplean en la obtención de productos basados en fermentación láctica y se ha reportado su actividad como probióticos. El objetivo del presente estudio fue identificar y comparar los elementos genómicos asociados a la formación de biopelículas en ambas subespecies utilizando herramientas computacionales. El análisis bibliométrico mostró que los genes de adhesinas, de síntesis de exopolisacáridos y elementos reguladores tipo cis y trans podrían estar asociados a la formación de biopelículas en ambas subespecies. La comparación de 12 genomas de L. delbrueckii subsp. bulgaricus y 7 de L. delbrueckii subsp. lactis detectaron la presencia de los genes pili y srtA en un operón conservado con porcentajes de identidad superiores al 97 %. También se identificó el gen inu que presenta función de levansucarasa. Finalmente se encontró el gen epsD, presente en una unidad transcripcional policistrónica, que codifica para una enzima asociada a la síntesis de heteropolisacáridos. Estos datos muestran por primera vez, evidencia de la presencia de genes que participan en la formación de biopelículas en cepas de L. delbrueckii subsp. bulgaricus y de L. delbrueckii subsp. lactis.
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