Caracterización estructural y funcional de nanopartículas de ZnO-EPS sintetizadas a partir de exopolisacáridos producidos por Lactiplantibacillus fabifermentans BAL-27-ITTG
Nanopartículas de ZnO-EPS
DOI:
https://doi.org/10.18633/biotecnia.v25i3.1950Palabras clave:
Nanopartículas, micropartículas, ZnO, Lactiplantibacillus fabifermentans, ExopolisacáridosResumen
Los exopolisacáridos (EPS) pueden ser utilizados como agentes reductores confiriendo mejores propiedades físicas y químicas a las nanopartículas metálicas. Este estudio tuvo como objetivo sintetizar y evaluar las características fisicoquímicas, estructurales y funcionales de nanopartículas de ZnO producidas con EPS de Lactiplantibacillus fabifermentans BAL-27-ITTG. La caracterización estructural de los EPS exhibió una estructura de alto peso molecular compuesta principalmente de manosa y glucosa con enlaces α-1,4, α-1,3, α-1,6 de acuerdo al espectro de RMN. El aumento de la concentración de EPS en la síntesis provoca el incremento en el tamaño de las partículas en escala micrométrica disminuyendo el índice de polidispersidad. La síntesis de las nanopartículas se confirmó mediante un barrido espectral UV-vis y el espectro FT-IR mostró picos de absorción a 600 cm-1 asociados al enlace Zn-O. Las nano y micropartículas sintetizadas mostraron actividades funcionales mejoradas respecto al EPS libre. Los valores máximos en actividad antioxidante (80.25 %), capacidad de retención de agua (3.06 g H2O/g muestra), actividad emulsionante (69.85%) y actividad floculante (69.07 %) se obtuvieron con las MpZnO-EPS sintetizadas con 6 mg/mL del agente reductor. Se observó actividad bacteriostática contra Staphylococcus aureus y L. fabifermentans. El ensayo de biocompatibilidad confirmó la obtención de partículas no hemolíticas.
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