Optimization of total anthocyanin content and antioxidant activity of a Hibiscus sabdariffa infusion using response surface methodology


  • Mayra L. Salmerón-Ruiz Centro de Investigación en Alimentación y Desarrollo A. C. Carretera Gustavo Enrique Astiazarán Rosas No. 46 Colonia La Victoria CP 83304. Hermosillo, Sonora, México
  • J. Abraham Domínguez-Avila Cátedras CONACYT-Centro de Investigación en Alimentación y Desarrollo A. C. Carretera Gustavo Enrique Astiazarán Rosas No. 46 Colonia La Victoria CP 83304. Hermosillo, Sonora, México
  • J. Fernando Ayala-Zavala Centro de Investigación en Alimentación y Desarrollo A. C. Carretera Gustavo Enrique Astiazarán Rosas No. 46 Colonia La Victoria CP 83304. Hermosillo, Sonora, México
  • Emilio Alvarez-Parrilla Universidad Autónoma de Ciudad Juárez. Anillo Envolvente del PRONAF y Estocolmo S/N. CP 32315. Ciudad Juárez, Chihuahua, México
  • Mónica A. Villegas-Ochoa Centro de Investigación en Alimentación y Desarrollo A. C. Carretera Gustavo Enrique Astiazarán Rosas No. 46 Colonia La Victoria CP 83304. Hermosillo, Sonora, México
  • Sonia Guadalupe Sáyago-Ayerdi Instituto Tecnológico de Tepic. Av. Tecnológico # 2295 Lagos del Country CP 63175. Tepic, Nayarit, México
  • Martín Valenzuela-Melendez Centro de Investigación en Alimentación y Desarrollo A. C. Carretera Gustavo Enrique Astiazarán Rosas No. 46 Colonia La Victoria CP 83304. Hermosillo, Sonora, México
  • Gustavo A. González-Aguilar Centro de Investigación en Alimentación y Desarrollo A. C. Carretera Gustavo Enrique Astiazarán Rosas No. 46 Colonia La Victoria CP 83304. Hermosillo, Sonora, México



Palabras clave:

Hibiscus, Roselle, Anthocyanin, Antioxidant, Response surface methodology


Hibiscus sabdariffa L. calyces are underutilized sources of health-promoting anthocyanins. Infusions are the most common way to consume them, but because anthocyanins are thermosensitive, prolonged extraction times at high temperatures may reduce their bioactivities, suggesting the need to identify optimal preparation conditions. Response surface methodology was used to establish calyces-to-water ratio (X1: 1–20 g/100 mL), temperature (X2: 70–100 °C), and time (X3: 1–30 min) that would produce an infusion with optimized total anthocyanin content (TAC) and antioxidant activity. Under optimum conditions (X1=10 g/100 mL, X2=88.7 °C, and X3=15.5 min) TAC was 132.7±7.8 mg cyanidin-3-glucoside equivalents (C3G)/100 mL, and antioxidant activity was 800.6±69.9 (DPPH assay), and 1792.0±153.5 (ABTS assay) μmol Trolox equivalents (TE)/100 mL. Predicted and experimental results were statistically similar. Identifying ideal processing conditions can promote consumption of an H. sabdariffa-based functional beverage with high anthocyanin content and antioxidant activity that exert health-promoting bioactivities on the consumer.


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Ahmad, M. A. and Alrozi, R. 2010. Optimization of preparation conditions for mangosteen peel-based activated carbons for the removal of Remazol Brilliant Blue R using response surface methodology. Chemical Engineering Journal, 165, 883–890.

Ali, B. H., Cahlikova, L., Opletal, L., Karaca, T., Manoj, P., Ramkumar, A., Al Suleimani, Y. M., Al Za’abi, M., Nemmar, A., Chocholousova-Havlikova, L., Locarek, M., Siatka, T. and Blunden, G. 2017. Effect of aqueous extract and anthocyanins of calyces of Hibiscus sabdariffa (Malvaceae) in rats with adenine-induced chronic kidney disease. Journal of Pharmacy and Pharmacology, 69, 1219–1229.

Ali, B. H., Wabel, N. A. and Blunden, G. 2005. Phytochemical, pharmacological and toxicological aspects of Hibiscus sabdariffa L.: a review. Phytotherapy Research, 19, 369–375.

Aurelio, D.-L., Edgardo, R. G. and Navarro-Galindo, S. 2008. Thermal kinetic degradation of anthocyanins in a roselle (Hibiscus sabdariffa L. cv. ‘Criollo’) infusion. International Journal of Food Science and Technology, 43, 322–325.

Bechoff, A., Cissé, M., Fliedel, G., Declemy, A.-L., Ayessou, N., Akissoe, N., Touré, C., Bennett, B., Pintado, M., Pallet, D. and Tomlins, K. I. 2014. Relationships between anthocyanins and other compounds and sensory acceptability of Hibiscus drinks. Food Chemistry, 148, 112–119.

Bolea, C., Turturica, M., Stanciuc, N. and Vizireanu, C. 2016. Thermal degradation kinetics of bioactive compounds from black rice flour (Oryza sativa L.) extracts. Journal of Cereal Science, 71, 160–166.

Chan, E., Lim, Y., Wong, S., Lim, K., Tan, S., Lianto, F. and Yong, M. 2009. Effects of different drying methods on the antioxidant properties of leaves and tea of ginger species. Food Chemistry, 113, 166–172.

Chiu, C. T., Chen, J. H., Chou, F. P. and Lin, H. H. 2015. Hibiscus sabdariffa Leaf Extract Inhibits Human Prostate Cancer Cell Invasion via Down-Regulation of Akt/NF-kappa B/MMP-9 Pathway. Nutrients, 7, 5065–5087.

Chumsri, P., Sirichote, A. and Itharat, A. 2008. Studies on the optimum conditions for the extraction and concentration of roselle (Hibiscus sabdariffa Linn.) extract. Songklanakarin Journal of Science and Technology, 30, 133–139.

Cisse, M., Dornier, M., Sakho, M., Ndiaye, A., Reynes, M. and Sock, O. 2009. Le bissap (Hibiscus sabdariffa L.): composition et principales utilisations. Fruits, 64, 179–193.

Corbo, M. R., Bevilacqua, A., Petruzzi, L., Casanova, F. P. and Sinigaglia, M. 2014. Functional Beverages: The Emerging Side of Functional Foods Commercial Trends, Research, and Health Implications. Comprehensive Reviews in Food Science and Food Safety, 13, 1192–1206.

Domínguez Avila, J. A., Wall Medrano, A., Ruiz Pardo, C. A., Montalvo González, E. and González Aguilar, G. A. 2018. Use of nonthermal technologies in the production of functional beverages from vegetable ingredients to preserve heat-labile phytochemicals. Journal of Food Processing and Preservation, 42, e13506.

Fan, G., Han, Y., Gu, Z. and Chen, D. 2008. Optimizing conditions for anthocyanins extraction from purple sweet potato using response surface methodology (RSM). LWT-Food Science and Technology, 41, 155–160.

Fernández-Arroyo, S., Rodríguez-Medina, I. C., Beltrán-Debón, R., Pasini, F., Joven, J., Micol, V., Segura-Carretero, A. and Fernández-Gutiérrez, A. 2011. Quantification of the polyphenolic fraction and in vitro antioxidant and in vivoanti-hyperlipemic activities of Hibiscus sabdariffa aqueous extract. Food Research International, 44, 1490–1495.

Galicia-Flores, L., Salinas-Moreno, Y., Espinoza-García, B. and Sánchez-Feria, C. 2008. Caracterización fisicoquímica y actividad antioxidante de extractos de Jamaica (Hibiscus sabdariffa L.) nacional e importada. Revista Chapingo Serie Horticultura, 14, 121–129.

Garba, Z. N. and Rahim, A. A. 2014. Process optimization of K2C2O4-activated carbon from Prosopis africana seed hulls using response surface methodology. Journal of Analytical and Applied Pyrolysis, 107, 306–312.

Gradinaru, G., Biliaderis, C. G., Kallithraka, S., Kefalas, P. and Garcia-Viguera, C. 2003. Thermal stability of Hibiscus sabdariffaL. anthocyanins in solution and in solid state: effects of copigmentation and glass transition. Food Chemistry, 83, 423–436.

Grajeda-Iglesias, C., Figueroa-Espinoza, M. C., Barouh, N., Barea, B., Fernandes, A., De Freitas, V. and Salas, E. 2016. Isolation and Characterization of Anthocyanins from Hibiscus sabdariffaFlowers. Journal of Natural Products, 79, 1709–1718.

Guldiken, B., Boyacioglu, D. and Capanoglu, E. 2016. Optimization of Extraction of Bioactive Compounds from Black Carrot Using Response Surface Methodology (RSM). Food Analytical Methods, 9, 1876–1886.

He, B., Zhang, L. L., Yue, X. Y., Liang, J., Jiang, J., Gao, X. L. and Yue, P. X. 2016. Optimization of Ultrasound-Assisted Extraction of phenolic compounds and anthocyanins from blueberry (Vaccinium ashei) wine pomace. Food Chemistry, 204, 70–76.

Herrera-Arellano, A., Flores-Romero, S., Chávez-Soto, M. A. and Tortoriello, J. 2004. Effectiveness and tolerability of a standardized extract from Hibiscus sabdariffa in patients with mild to moderate hypertension: a controlled and randomized clinical trial. Phytomedicine, 11, 375–382.

Ku, C. S. and Mun, S. P. 2008. Optimization of the extraction of anthocyanin from Bokbunja (Rubus coreanus Miq.) marc produced during traditional wine processing and characterization of the extracts. Bioresourse Technology, 99, 8325–8330.

Lee, J., Durst, R. W. and Wrolstad, R. E. 2005. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study. Journal of AOAC International, 88, 1269–1278.

Lin, T.-L., Lin, H.-H., Chen, C.-C., Lin, M.-C., Chou, M.-C. and Wang, C.-J. 2007. Hibiscus sabdariffa extract reduces serum cholesterol in men and women. Nutrition Research, 27, 140–145.

Malacrida, A., Maggioni, D., Cassetti, A., Nicolini, G., Cavaletti, G. y Miloso, M. 2016. Antitumoral Effect of Hibiscus sabdariffa on Human Squamous Cell Carcinoma and Multiple Myeloma Cells. Nutrition and Cancer-an International Journal, 68, 1161–1170.

Martins, A. C., Bukman, L., Vargas, A. M., Barizão, É. O., Moraes, J. C., Visentainer, J. V. and Almeida, V. C. 2013. The antioxidant activity of teas measured by the FRAP method adapted to the FIA system: Optimising the conditions using the response surface methodology. Food Chemistry, 138, 574–580.

Mckay, D. L., Chen, C.-Y. O., Saltzman, E. and Blumberg, J. B. 2010. Hibiscus Sabdariffa L. Tea (Tisane) lowers blood pressure in prehypertensive and mildly hypertensive adults. The Journal of Nutrition, 140, 298–303.

Montgomery, D. C. 1997. Design and analysis of experiments,New York, Wiley.

Myer, R. and Montgomery, D. C. 2002. Response Surface Methodology: process and product optimization using designed experiment,New York, Wiley.

Nath, A. and Chattopadhyay, P. 2007. Optimization of oven toasting for improving crispness and other quality attributes of ready to eat potato-soy snack using response surface methodology. Journal of Food Engineering, 80, 1282–1292.

Oboh, G. and Rocha, J. B. T. 2008. Antioxidant and neuroprotective properties of sour tea (Hibiscus sabdariffa, calyx) and green tea (Camellia sinensis) on some pro-oxidant-induced lipid peroxidation in brain in vitro. Food Biophysics, 3, 382–389.

Olatunji, L. A., Adebayo, J. O., Oguntoye, O. B., Olatunde, N. O., Olatunji, V. A. and Soladoye, A. O. 2005. Effects of aqueous extracts of petals of red and green Hibiscus sabdariffaon plasma lipid and hematological variables in rats. Pharmaceutical Biology, 43, 471–474.

Patras, A., Brunton, N. P., Da Pieve, S. and Butler, F. 2009. Impact of high pressure processing on total antioxidant activity, phenolic, ascorbic acid, anthocyanin content and colour of strawberry and blackberry purées. Innovative Food Science and Emerging Technologies, 10, 308–313.

Peron, D. V., Fraga, S. and Antelo, F. 2017. Thermal degradation kinetics of anthocyanins extracted from jucara (Euterpe edulis Martius) and “Italia” grapes (Vitis vinifera L.), and the effect of heating on the antioxidant capacity. Food Chemistry, 232, 836–840.

Pinelo, M., Rubilar, M., Jerez, M., Sineiro, J. and Núñez, M. J. 2005. Effect of solvent, temperature, and solvent-to-solid ratio on the total phenolic content and antiradical activity of extracts from different components of grape pomace. Journal of Agricultural and Food Chemistry, 53, 2111–2117.

Prenesti, E., Berto, S., Daniele, P. G. and Toso, S. 2007. Antioxidant power quantification of decoction and cold infusions of Hibiscus sabdariffa flowers. Food Chemistry, 100, 433–438.

Ramirez-Rodrigues, M. M., Plaza, M. L., Azeredo, A., Balaban, M. O. and Marshall, M. R. 2011. Physicochemical and phytochemical properties of cold and hot water extraction from Hibiscus sabdariffa. Journal of Food Science, 76, 428–435.

Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231–1237.

Rhim, J.-W. 2002. Kinetics of thermal degradation of anthocyanin pigment solutions driven from red flower cabbage. Food Science and Biotechnology, 11, 361–364.

Sáyago-Ayerdi, S. G., Arranz, S., Serrano, J. and Goñi, I. 2007. Dietary fiber content and associated antioxidant compounds in roselle flower (Hibiscus sabdariffa L.) beverage. Journal of Agricultural and Food Chemistry, 55, 7886–7890.

Senthilkumar, S. R., Ashokkumar, B., Chandra Raj, K. and Gunasekaran, P. 2005. Optimization of medium composition for alkali-stable xylanase production by Aspergillus fischeriFxn 1 in solid-state fermentation using central composite rotary design. Bioresourse Technology, 96, 1380–1386.

Serban, C., Sahebkar, A., Ursoniu, S., Andrica, F. and Banach, M. 2015. Effect of sour tea (Hibiscus sabdariffa L.) on arterial hypertension: a systematic review and meta-analysis of randomized controlled trials. Journal of Hypertension, 33, 1119–1127.

Setford, P. C., Jeffery, D. W., Grbin, P. R. and Muhlack, R. A. 2017. Factors affecting extraction and evolution of phenolic compounds during red wine maceration and the role of process modelling. Trends in Food Science & Technology, 69, 106–117.

Simic, V. M., Rajkovic, K. M., Stojicevic, S. S., Veliclovic, D. T., Nikolic, N. C., Lazic, M. L. and Karabegovic, I. T. 2016. Optimization of microwave-assisted extraction of total polyphenolic compounds from chokeberries by response surface methodology and artificial neural network. Separation and Purification Technology, 160, 89–97.

Sin, H. N., Yusof, S., Sheikh Abdul Hamid, N. and Rahman, R. A. 2006. Optimization of enzymatic clarification of sapodilla juice using response surface methodology. Journal of Food Engineering, 73, 313–319.

Sinela, A., Rawat, N., Mertz, C., Achir, N., Fulcrand, H. and Dornier, M. 2017. Anthocyanins degradation during storage of Hibiscus sabdariffa extract and evolution of its degradation products. Food Chemistry, 214, 234–241.

Spigno, G. and De Faveri, D. M. 2007. Antioxidants from grape stalks and marc: Influence of extraction procedure on yield, purity and antioxidant power of the extracts. Journal of Food Engineering, 78, 793–801.

Thoo, Y. Y., Ho, S. K., Liang, J. Y., Ho, C. W. and Tan, C. P. 2010. Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia). Food Chemistry, 120, 290–295.

Tsai, P.-J., Mcintosh, J., Pearce, P., Camden, B. and Jordan, B. R. 2002. Anthocyanin and antioxidant capacity in roselle (Hibiscus Sabdariffa L.) extract. Food Research International, 35, 351–356.

Vahid Farzaneh and Carvalho, I. S. 2017. Modelling of Microwave Assisted Extraction (MAE) of Anthocyanins (TMA). Journal of Applied Research on Medicinal and Aromatic Plants, 6, 92–100.

Villa-Rodríguez, J. A., Molina-Corral, F. J., Ayala-Zavala, J. F., Olivas, G. I. and González-Aguilar, G. A. 2011. Effect of maturity stage on the content of fatty acids and antioxidant activity of ‘Hass’ avocado. Food Research International, 44, 1231–1237.

Wong, P. K., Yusof, S., Ghazali, H. M. and Man, C. 2003. Optimization of hot water extraction of roselle juice using response surface methodology: a comparative study with other extraction methods. Journal of the Science of Food and Agriculture, 83, 1273–1278.




Cómo citar

Salmerón-Ruiz, M. L., Domínguez-Avila, J. A., Ayala-Zavala, J. F., Alvarez-Parrilla, E., Villegas-Ochoa, M. A., Sáyago-Ayerdi, S. G., … González-Aguilar, G. A. (2019). Optimization of total anthocyanin content and antioxidant activity of a Hibiscus sabdariffa infusion using response surface methodology. Biotecnia, 21(2), 114–122. https://doi.org/10.18633/biotecnia.v21i2.937



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