CONCENTRATION OF STEVIOSIDE AND REBAUDIOSIDE-A IN Stevia rebaudiana BERTONI PLANTS IN INTERACTION WITH ARBUSCULAR MYCORRHIZAE AND PHOSPHORUS

Authors

  • Rodrigo A. Cauich-Cauich
  • Emanuel Hernández-Núñez cinvestav
  • MONICA GUADALUPE LOZANO-CONTRERAS INIFAP https://orcid.org/0000-0002-5582-7799
  • José María Tun-Suárez
  • Hugo Pilotzi-Xahuentitla cinvestav
  • Rubén Humberto Andueza-Noh National Technological Institute of Mexico
  • Carlos Juan Alvarado-López National Technological Institute of Mexico

DOI:

https://doi.org/10.18633/biotecnia.v26.2280

Keywords:

metabolites, glycosides, AM, phosphate, quantification

Abstract

Stevia rebaudiana produces steviol glycosides in its leaves, stevioside and rebaudioside-A are the most important thanks to their sweetening property. Phosphorus is an essential element in the biosynthesis of steviol glycosides and arbuscular mycorrhizae can promote the concentration of glycosides by facilitating the translocation of phosphorus to the plant. Therefore, the concentration of stevioside and rebaudioside-A was quantified by means of 1H-NMR in S. rebaudiana plants inoculated with arbuscular mycorrhizal consortia (RC, TZ and CY consortia) and fertilized with phosphorus levels (25, 50, 75 and 100 %). A 76 % mycorrhizal colonization was obtained with the RC + 25 % phosphorus treatment, where a higher leaf phosphorus content and a higher concentration of stevioside and rebaudioside-A in mg g-1 of dry leaf were also recorded, significantly surpassing conventional fertilization. With this combination, it was possible to improve the yield of stevioside and rebaudioside-A in S. rebaudiana leaves. Furthermore, it is highlighted that the 1H-NMR method was used as a viable alternative to HPLC to quantify the main steviol glycosides in S. rebaudiana, which provides quantitative results in less time and with the same precision. 

 

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Author Biographies

Rodrigo A. Cauich-Cauich

M.C. Rodrigo A. Cauich-Cauich, graduate of the National Technological Institute of Mexico, Conkal Campus, with a Master's degree in Tropical Horticulture Sciences, author of 4 scientific articles related to Stevia rebaudiana.

Emanuel Hernández-Núñez, cinvestav

Dr. Emanuel Hernández Núñez, CONACYT Chair Researcher, from the Department of Marine Resources at CINVESTAV Mérida, SNI: Level 1. Research focus: Design and synthesis of New Chemical Entities with potential biological activity. Development and validation of analytical methods for the identification and quantification of contaminants, secondary metabolites, and biological markers.

MONICA GUADALUPE LOZANO-CONTRERAS, INIFAP

Senior Researcher C at the National Institute of Forestry, Agriculture, and Livestock Research (INIFAP) since 2008, graduated from the Faculty of Biological Sciences at the Autonomous University of Nuevo León, with a postdoctoral fellowship at the Monterrey Institute of Technology and Higher Education (ITESM) (2007-2008). Member of the National Researchers System of CONACYT, Level 1. Research lines: use and management of biofertilizers, as well as bioinputs and their effectiveness in various crops of agricultural importance.

José María Tun-Suárez

José María Tun Suárez, Ph.D. in Plant Pathology, Researcher at the National Technological Institute of Mexico, Conkal Campus. SNI: Level 1. Research focus: Diagnosis and control of diseases in tropical horticultural crops. Emphasis on the use of antagonist microorganisms in the control of foliar phytopathogenic fungi, and microorganisms inducing resistance to phytopathogenic viruses in plants.

Hugo Pilotzi-Xahuentitla, cinvestav

Dr. Hugo Pilotzi-Xahuentitla, Postdoctoral Researcher at the Department of Marine Resources, CINVESTAV Mérida, SNI: Level 1. Research focus: Development and validation of analytical methods for the identification and quantification of contaminants, secondary metabolites, and biological markers.

Rubén Humberto Andueza-Noh, National Technological Institute of Mexico

Rubén Humberto Andueza-Noh, Ph.D. in Tropical Horticultural Sciences, CONACYT Chair Researcher at the National Technological Institute of Mexico, Conkal Campus. SNI: Level 1. Research focus: Management of tropical horticultural crops.

Carlos Juan Alvarado-López, National Technological Institute of Mexico

Dr. Carlos Juan Alvarado López, CONACYT Professor at the National Technological Institute of Mexico, Conkal Campus. SNI: Level 1. Research focus: Diagnosis and elemental analysis of soils and their interactions in Tropical Horticulture, Isolation, identification, and application of root symbiotic microorganisms for use in horticultural systems.

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2024-11-27

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Cauich-Cauich, R. A., Hernández-Núñez, E., LOZANO CONTRERAS, M. G., Tun-Suárez, J. M., Pilotzi-Xahuentitla, H., Andueza-Noh, R. H., & Alvarado-López, C. J. (2024). CONCENTRATION OF STEVIOSIDE AND REBAUDIOSIDE-A IN Stevia rebaudiana BERTONI PLANTS IN INTERACTION WITH ARBUSCULAR MYCORRHIZAE AND PHOSPHORUS . Biotecnia, 26, e2280. https://doi.org/10.18633/biotecnia.v26.2280

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Vol. 26 (2024): January - December

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Research Articles

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