Prosthechea karwinskii leaves extract with potential for the treatment of atherothrombosis
DOI:
https://doi.org/10.18633/biotecnia.v26.2378Keywords:
anticoagulant, cellular antioxidant, reactive oxygen speciesAbstract
Prosthechea karwinskii is an orchid endemic to southeastern Mexico used in traditional medicine. It has anti-inflammatory, antioxidant and cardioprotective activity. Given the relationship between inflammation, oxidative stress, and coagulation in the development of atherothrombosis, the objective of this research was to evaluate the potential of P. karwinskii leaves extract as a possible alternative to reduce the risk of atherothrombosis. The compounds of the extract were identified by UHPLC-ESI-qTOF-MS. The content of total phenols and flavonoids was measured, as well as its antioxidant capacity (DPPH and DCFH-DA essays) and its effect on clotting times. The results showed the presence of phenolic and flavonoid compounds in the extract, as well as their antioxidant capacity. In addition, the extract prolonged clotting times, mainly thrombin, and activated partial thromboplastin times, i.e., it inhibited the intrinsic pathway of hemostasis and the conversion of fibrinogen to fibrin. These results and the background of the extract show its potential as a treatment to reduce atherothrombotic risk, as well as for other diseases whose pathogenesis involves oxidative stress and coagulation.
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Avula, B., Katragunta, K., Wang, Y.H., Ali, Z., Srivedavyasasri, R., Gafner, S., Slimestad, R., Khan, I.A., 2022. Chemical profiling and UHPLC-QToF analysis for the simultaneous determination of anthocya-nins and flavonoids in Sambucus berries and authentication and detection of adulteration in elderberry dietary supplements using UHPLC-PDA-MS. Journal of Food Composition and Analysis 110, 104584. https://doi.org/10.1016/j.jfca.2022.104584
Barragán-Zarate, G.S., Alexander-Aguilera, A., Lagunez-Rivera, L., Solano, R., Soto-Rodríguez, I. 2021. Bioactive compounds from Prosthechea karwinskii decrease obesity, insulin resistance, pro-inflammatory status, and cardiovascular risk in Wistar rats with metabolic syndrome. Journal of Ethnopharmacology 114376. https://doi.org/10.1016/j.jep.2021.114376
Barragán-Zarate, G.S., Lagunez-Rivera, L., Solano, R., Carranza-Álvarez, C., Hernández-Benavides, D.M., Vilarem, G. 2022. Validation of the traditional medicinal use of a Mexican endemic orchid (Prosthechea karwinskii) through UPLC-ESI-qTOF-MS/MS characterization of its bioactive compounds. Heliyon 8. https://doi.org/10.1016/j.heliyon.2022.e09867
Barragán-Zarate, G.S., Lagunez-Rivera, L., Solano, R., Pineda-Peña, E.A., Landa-Juárez, A.Y., Chávez-Piña, A.E., Carranza-Álvarez, C., Hernández-Benavides, D.M. 2020. Prosthechea karwinskii, an orchid used as traditional medicine, exerts anti-inflammatory activity and inhibits ROS. Journal of Ethnopharma-cology 253, 112632. https://doi.org/10.1016/j.jep.2020.112632
Bedir, F., Kocatürk, H., Yapanoğlu, T., Gürsul, C., Arslan, R., Mammadov, R., Çoban, A., Altuner, D., Suleyman, H., 2021. Protective effect of taxifolin against prooxidant and proinflammatory kidney damage associated with acrylamide in rats. Biomedicine and Pharmacotherapy 139. https://doi.org/10.1016/j.biopha.2021.111660
Chan, N.C., Xu, K., de Vries, T.A.C., Eikelboom, J.W., Hirsh, J. 2022. Inflammation as a Mechanism and Therapeutic Target in Peripheral Artery Disease. Canadian Journal of Cardiology 38, 588–600. https://doi.org/10.1016/j.cjca.2022.01.026
Chang, C., Yang, M., Wen, H., and Chern, J. 2002. Estimation of total flavonoids content in propolis by two complementary colorimetric methods. J. Food Drug Anal. 10: 176-182.
Choi, J.H., Kim, D.W., Park, S.E., Lee, H.J., Kim, K.M., Kim, K.J., Kim, M.K., Kim, S.J., Kim, S., 2015. Anti-thrombotic effect of rutin isolated from Dendropanax morbifera Leveille. Journal of Bioscience and Bioengineering 120, 181–186. https://doi.org/10.1016/j.jbiosc.2014.12.012
Cruz-Garcia, G., Solano-Gomez, R., Lagunez-Rivera, L. 2014. Documentation of the medicinal knowledge of Prosthechea karwinskii in a Mixtec community in Mexico. Revista Brasileira de Farmacognosia 24, 153–158. https://doi.org/10.1016/j.bjp.2014.03.002
Edziri, H., Guerrab, M., Anthonissen, R., Mastouri, M., Verschaeve, L., 2020. Phytochemical screening, antioxidant, anticoagulant and in vitro toxic and genotoxic properties of aerial parts extracts of Fumaria officinalis L. growing in Tunisia. South African Journal of Botany 130, 268–273. https://doi.org/10.1016/j.sajb.2020.01.014
Lagunez-Rivera, L., Barragan-Zarate, G. S., Solano, R., Alexander-Aguilera, A. and Chavez-Piña, A. E. 2023. Mexican Orchid (Prosthechea karwinskii) and Use in Cardiovascular Protection: Cellular and Physio-logical Aspects. In book: Ancient and Traditional Foods, Plants, Herbs and Spices used in Cardiovascular Health and Disease. Taylor and Francis.
Le, H.L., Nguyen, T.M.H., Vu, T.T., Nguyen, T.T.O., Ly, H.D.T., Le, N.T., Nguyen, V.H., Nguyen, T.V.A. 2022. Potent antiplatelet aggregation, anticoagulant and antioxidant activity of aerial Canna x generalis L.H Bailey & E.Z Bailey and its phytoconstituents. South African Journal of Botany 147, 882–893. https://doi.org/10.1016/j.sajb.2022.03.035
Leite, P.M., Miranda, A.P.N., Gomes, I., Rodrigues, M.L., Camargos, L.M., Amorim, J.M., Duarte, R.C.F., Faraco, A.A.G., Carvalho, M.G., Castilho, R.O., 2023. Antithrombotic potential of Lippia alba: A mechanistic approach. Journal of Ethnopharmacology 301. https://doi.org/10.1016/j.jep.2022.115744
López-Pérez, A., Hernández-Juárez, J., Solano, R., Majluf-Cruz, A., Hernández-Cruz, P.A., Lagunez-Rivera, L., 2022. Laelia furfuracea Lindl.: an Endemic Mexican Orchid with Anticoagulant Activity. Journal of the Mexican Chemical Society 66. https://doi.org/10.29356/jmcs.v66i1.1588
Mastenbroek, T.G., van Geffen, J.P., Heemskerk, J.W.M., Cosemans, J.M.E.M., 2015. Acute and persistent platelet and coagulant activities in atherothrombosis. Journal of Thrombosis and Haemostasis 13, S272–S280. https://doi.org/10.1111/jth.12972
Nguyen, A.B., Iqbal, O., Block, R.C., Mousa, S.A. 2023. Prevention and treatment of atherothrombosis: Potential impact of nanotechnology. Vascular Pharmacology 148, 107127. https://doi.org/10.1016/j.vph.2022.107127
Olie, R.H., van der Meijden, P.E.J., ten Cate, H., 2018. The coagulation system in atherothrombosis: Im-plications for new therapeutic strategies. Research and Practice in Thrombosis and Haemostasis 2, 188–198. https://doi.org/10.1002/rth2.12080
Padilla, R., Gaillard, V., Le, T.N., Bellvert, F., Chapulliot, D., Nesme, X., Dessaux, Y., Vial, L., Lavire, C., Kerzaon, I., 2021. Development and validation of a UHPLC-ESI-QTOF mass spectrometry method to analyze opines, plant biomarkers of crown gall or hairy root diseases. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 1162. https://doi.org/10.1016/j.jchromb.2020.122458
Pandey, K.B., Rizvi, S.I., 2009. Plant polyphenols as dietary antioxidants in human health and disease. Ox-idative Medicine and Cellular Longevity 2, 270–278. https://doi.org/10.4161/oxim.2.5.9498
Scherer, R. & Godoy, H.T. 2009. Antioxidant activity index (AAI) by the 2,2-diphenyl-1-picrylhydrazyl method. Food Chemistry,112: 654–658.
Shen, S., Wang, J., Chen, X., Liu, T., Zhuo, Q., Zhang, S.Q., 2019. Evaluation of cellular antioxidant components of honeys using UPLC-MS/MS and HPLC-FLD based on the quantitative composi-tion-activity relationship. Food Chemistry 293, 169–177. https://doi.org/10.1016/j.foodchem.2019.04.105
Shi, Y. ping, Zhang, Y. gang, Li, H. nan, Kong, H. tian, Zhang, S. shan, Zhang, X. ming, Li, X. bin, Liu, K. chun, Han, L. wen, Tian, Q. ping, 2020. Discovery and identification of antithrombotic chemical markers in Gardenia Fructus by herbal metabolomics and zebrafish model. Journal of Ethnopharmacology 253, 112679. https://doi.org/10.1016/j.jep.2020.112679
Swain, T., HillisWE. 1959. The phenolic constituents of Prunus domestica. I. The quantitative analysis of phenolic constituents. J Sci Food Agric 10:63–8.
Tang, X., Cui, Y., Feng, B., 2024. The chemical constituents and metabolite profiles of Huangqin decoction in normal and ulcerative colitis rats by UHPLC-Q-TOF/MS analysis. Journal of Pharmaceutical and Bi-omedical Analysis 237, 115763. https://doi.org/10.1016/j.jpba.2023.115763
Torres-Rodríguez, M.L., García-Chávez, E., Berhow, M., De Mejia, E.G. 2016. Anti-in flammatory and anti-oxidant effect of Calea urticifolia lyophilized aqueous extract on lipopolysaccharide-stimulated RAW 264.7 macrophages. J. Ethnopharmacol. 188, 266–274. https://doi.org/10.1016/j.jep.2016.04.057.
Wang, S., Yao, W., Zhu, X., Wang, J., Lu, L., Zhu, N., Lan, T., Kuang, Y., Zhu, W., Liu, R., Huang, L., 2023. Exploring the mechanism of the antithrombotic effects of Pueraria lobata and Pueraria lobata var. thomsonii based on network pharmacology. Journal of Ethnopharmacology 300, 115701. https://doi.org/10.1016/j.jep.2022.115701
Wang, Y., Tang, C., Zhang, H., 2015. Hepatoprotective effects of kaempferol 3-O-rutinoside and kaempferol 3-O-glucoside from Carthamus tinctorius L. on CCl4-induced oxidative liver injury in mice. Journal of Food and Drug Analysis 23, 310–317. https://doi.org/10.1016/j.jfda.2014.10.002
Wolfe, K.L., Rui, H.L., 2007. Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements. Journal of Agricultural and Food Chemistry 55, 8896–8907. https://doi.org/10.1021/jf0715166
Yeh, C.H., Yang, J.J., Yang, M.L., Li, Y.C., Kuan, Y.H., 2014. Rutin decreases lipopolysaccharide-induced acute lung injury via inhibition of oxidative stress and the MAPK-NF-κB pathway. Free Radical Biology and Medicine 69, 249–257. https://doi.org/10.1016/j.freeradbiomed.2014.01.028
Yin, Q., Zhang, X., Liao, S., Huang, X., Wan, C.C., Wang, Y. 2023. Potential anticoagulant of traditional chinese medicine and novel targets for anticoagulant drugs. Phytomedicine 116, 154880. https://doi.org/10.1016/j.phymed.2023.154880
Zaragozá, C., Monserrat, J., Mantecón, C., Villaescusa, L., Álvarez-Mon, M.Á., Zaragozá, F., Álvarez-Mon, M., 2021. Binding and antiplatelet activity of quercetin, rutin, diosmetin, and diosmin flavonoids. Bi-omedicine and Pharmacotherapy 141. https://doi.org/10.1016/j.biopha.2021.111867
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