Recovery of ellagitannins from Eucalyptus camaldulensis: Comparison of conventional and emerging hybrid technologies, its antioxidant and antigiardia effect
DOI:
https://doi.org/10.18633/biotecnia.v27.2503Keywords:
Extracts, Giardia lamblia, Maceration, Ultrasound-MicrowaveAbstract
Eucalyptus camaldulensis is a plant that offers phenolic compounds with antioxidant and antimicrobial properties. This study aimed to compare conventional and emerging extraction techniques to recover a set of ellagitannins and evaluate their antioxidant and antiparasitic activities. Three extraction methods were employed under different conditions to determine the most effective method for obtaining phenolic compounds. The extraction yielding the highest number of phenolic compounds was subjected to purification, phenolic profile identification, antioxidant potential evaluation, and antiparasitic activity against Giardia lamblia. Results indicated that ultrasound-microwave assisted extraction method was the most effective, yielding seven compounds, predominantly ellagitannins. In addition, it allowed to obtain an antioxidant activity on DPPH of an IC₅₀ of 371.13 mg/L, for lipid oxidation inhibition assay (LOI) IC₅₀ 173.09 mg/L, for the FRAP assay content of about 500 mq equivalents of Trolox/L, and ABTS IC₅₀ 25.28 mg/L. In addition, ellagitannins succeeded in inhibiting Giardia lamblia, reaching a maximum activity of around 80 % at 48 h. This suggests that hybrid extraction is effective for obtaining ellagitannins with antioxidant potential and antiparasitic activity from Eucalyptus camaldulensis.
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