VARIABILIDAD GENÉTICA EN ADN MICROSATÉLITE DE UN NUEVO LINAJE DE OSTIÓN (Crassostrea gigas) EN SONORA
DOI:
https://doi.org/10.18633/bt.v15i1.130Palabras clave:
Crassostrea gigas, Crassostrea corteziensis, acuacultura, microsatélites, genética de poblacionesResumen
Se evaluó la variabilidad genética en seis loci microsatelitales de dos generaciones consecutivas de un nuevo linaje de Crassostrea gigas que se pretende establecer en los cultivos del Golfo de California. Se detectaron un total de 66 alelos y 146 genotipos en la muestra del conjunto parental y 68 alelos y 168 genotipos en la muestra de la generación F1. El promedio de la heterocigosis observada fue de 0,65 para la generación parental y de 0,67 para la F1, sin diferencias significativas entre ellas. Todos los loci mostraron una deficiencia de heterocigotos con excepción de ucdCg10 en la F1, pero no se encontró evidencia de una endogamia acumulada. Se encontraron diferencias en la distribuciones de las frecuencias alélicas y genotípicas de cinco loci. El monitoreo de la variabilidad genética de organismos provenientes de laboratorios de producción no es un análisis de rutina en el proceso del control de calidad en la mayoría de los laboratorios. El análisis de microsatélites es una buena herramienta para vigilar las fluctuaciones de la heterocigosis a lo largo de la vida productiva de los linajes de cultivo.Descargas
Citas
Carlsson, J. y Reece, K.S. 2007. Eight PCR primers to amplify EST-linked microsatellites in the Eastern oyster, Crassostrea virginica genome. Molecular Ecology Notes. 7: 257–259.
Cenic, J.L. 2000. Nuevas técnicas moleculares para la identificación varietal de plantas. Revista Terralia. 12: 40-43.
Cornuet, J.M. y Luikart, G. 1996. Description and evaluation of two tests for detecting recent bottlenecks. Genetics. 144: 2001-2014.
Cruz, P., Yañez-Jacome, B., Ibarra, A.M. y Rangel-Becerril, J. 2007. Isolation and characterization of microsatellite loci in the Pacific pleasure oyster, Crassostrea corteziensis, and their cross-species amplification in four other oyster species. Molecular Ecology Notes. 7: 448–450.
De la Rosa-Vélez, J., Gutiérrez-Wing, M.T. y Radilla-Camacho, R. 1991. El ostricultivo de Bahía de San Quintín, B.C. México; Aspectos genéticos. Ciencias Marinas. 17: 133-147.
Enríquez-Espinoza, T.L. y Grijalva-Chon, J.M. 2010. Genetic variability of Crassostrea gigas and Crassostrea corteziensis from a hatchery in northwestern Mexico. Ciencias Marinas. 36: 333-344.
Frankham, R. 1996. Relationship of genetic variation to population size in wildlife. Conservation Biology. 10: 1500-1508.
Freitas, P.D. y Galetti Jr., P.M. 2005. Assessment of the genetic diversity in five generations of a commercial broodstock line of Litopenaeus vannamei shrimp. African Journal of Biotechnology.4: 1362-1367.
Gaffney, P.M. 1990. Enzyme heterozygosity, growth rate and viability in Mytilus edulis: another look. Evolution. 44: 204–210.
Geist, J., Rottmann, O., Schröder, W. y Kühn, R. 2003. Development of microsatellite markers for the endangered freshwater pearl mussel Margaritifera margaritifera L. (Bivalvia: Unionoidea) Molecular Ecology Notes. 3:444–446.
Huvet, A., Boudry, P., Ohresser, M., Delsert, C. y Bonhomme, F. 2000. Variable microsatellites in the Pacific Oyster Crassostrea gigas and other cupped oyster species. Animal Genetics. 31: 71-72.
Islas, O.R. 1975. El ostión japonés (Crassostrea gigas) en Baja California. Ciencias Marinas. 2: 58-59.
Kalinowski, S.T. 2006. HW-QUICKCHECK: an easy-to-use computer program for checking genotypes for agreement with Hardy-Weinberg expectation. Molecular Ecology Notes. 6: 974-979.
Launey, S. y Hedgecock, D. 2001. High genetic load in the Pacific oyster Crassostrea gigas. Genetics. 159: 255–265.
Li, C.C. y Horvitz, D.G. 1953. Some methods of estimating the inbreeding coefficient. American Journal of Human Genetics. 5: 107–117.
Li, G., Hubert, S., Bucklin, K., Ribes, V. y Hedgecock, D. 2003. Characterization of 79 microsatellite DNA markers in the Pacific oyster Crassostrea gigas. Molecular Ecology Notes. 3: 228–232.
Li, Q., Yu, H. y Yu, R. 2006. Genetic variability assessed by microsatellites in cultured populations of the Pacific oyster (Crassostrea gigas) in China. Aquaculture. 259: 95–102.
Liu, H.P. y Hershler, R. 2009. Genetic diversity and population structure of the threatened Bliss Rapids snail (Taylorconcha serpenticola). Freshwater Biology. 54: 1285-1299.
Magoulas, A., Gjetvag, B., Terzoglou, V. y Zouros, E. 1998. The polymorphic microsatellites in the Japanese oyster, Crassostrea gigas (Thunberg). Animal Genetics. 29: 69-70.
McGoldbrick, D.J., Hedgecock, D., English, L., Baoprasertkul, P. y Ward, R.D. 2000. The transmission of microsatellite alleles in Australian and North American stocks of the Pacific oyster (Crassostrea gigas): selection and null alleles. Journal of Shellfish Research. 19: 779-788.
Milligan, B.G., Leebens-Mack, J. y Strand, A.E. 1994. Conservation genetics: beyond the maintenance of marker diversity. Molecular Ecology. 3: 423-435.
Nei, M. 1978. Estimation of average heterozygosity and genetic from a small number of individuals. Genetics. 89: 583-590.
Ozaki, H. y Fujio, Y. 1985. Genetic differentiation in geographical populations of the Pacific Oyster (Crassostrea gigas) around Japan. Tohoku Journal of Agriculture Research. 36: 49-61.
Peakall, R. y Smouse, P.E. 2006. GenAlEx6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes. 6: 288-295.
Piry, S., Luikart, G. y Cornuet, J.M. 1999. Bottleneck: a computer for detecting recent reductions in the effective population size using allele frequency data. Journal of Heredity. 90: 502-503.
Raymond, M. y Rousset, F. 1995. GENEPOP (version 4): population genetics software for exact tests and ecumenicism. Journal of Heredity. 86: 248–249.
Rivera-García, M. y Grijalva-Chon, J.M. 2006. Genetic variability and differentiation in cultured white shrimp Penaeus (Litopenaeus) vannamei with low and high growth. Ciencias Marinas, 32(1A): 1-11.
Sauvage, C., Boudry, P. y Lapégue, S. 2009. Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas. Molecular Ecology Resources. 9: 853-855.
Sbordoni, V., de la Rosa, G., Mattoccia, M., Cobolli-Sbordoni, M. y de Matthaeis, E. 1987. Genetic changes in seven generations of hatchery stock of the kuruma prawn, Penaeus japonicus (Crustacea, decapoda). En Proceedings of the World Symposium on Selection, Hybridization, and Genetic Engineering in Acuaculture. K. Tiews (ed.), pp 143-155. Burdeos, Francia, 27–30 Mayo, 1986.
Sekino, M., Hamaguchi, M., Aranishi, F. y Okoshi, K. 2003. Development of novel microsatellite DNA markers from the Pacific oyster Crassostrea gigas. Marine Biotechnology. 5: 227-233.
Swofford, D. y Selander, R. 1981. BIOSYS-1: A Fortran program for the comprehensive analysis of electrophoresis data in population genetic and systematics. Journal of Heredity. 72: 281-283.
Tautz, D. 1989. Hypervariability of simple sequence as a general source for polimorphic DNA markers. Nucleic Acids Research. 17: 6463-6471.
Workman, P.L. y Niswander, J.D. 1970. Population studies on southwestern Indian Tribes. II Local genetic differentiation in the Papago. American Journal of Human Genetics. 22: 24–29.
Xiao, J., Cordes, J.F., Jones, K.C. y Reece, K.S. 2008. Eleven novel microsatellite markers for the Asian oyster Crassostrea ariakensis. Molecular Ecology Resources. 8: 843-845.
Yang, R., Yu, Z., Chen, Z., Kong, X. y Dai, J. 2000. Allozyme variation within Crassostrea plicatula and Crassostrea gigas from Shandong coastal waters. Fisheries China. 24: 130-133.
Yu, H., Li, Q. y Yu, R. 2008. Genetic differentiation between the oyster Crassostrea plicatula and Pacific oyster Crassostrea gigas populations in China assessed by microsatellite analysis. Fisheries Science. 74: 88–97.
Yu, Z. y Guo, X. 2005. Genetic analysis of selected strains of eastern oyster (Crassostrea virginica Gmelin) using AFLP and microsatellite markers. Marine Biotechnology. 6: 575-586.
Zane, L., Bargelloni, L y Patarnello, T. 2002. Strategies for microsatellite isolation: a review. Molecular Ecology. 11: 1-16.
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
La revista Biotecnia se encuentra bajo la licencia Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)
_(2).jpg)
