Biochemical and morphometric changes in Coffea arabica after a treatment with ethyl methanosulfonate

Biochemical and morphometric changes in Coffea arabica

Authors

DOI:

https://doi.org/10.18633/biotecnia.v25i3.1969

Keywords:

mutagenesis, EMS, genetic variability, secondary metabolism

Abstract

The use of ethyl methanesulfonate (EMS) is a technology used to increase the genetic variability of cultivars. In coffee genetic improvement, EMS could generate new phenotypic and biochemical traits of agricultural importance. The objective of this research was to determine the changes in morphology and production of secondary metabolites in coffee plants generated from cell lines treated with EMS. Callus of Coffea arabica cv., Borbon were exposed to nine concentrations of EMS, and subsequently, the percentage of survival and fresh biomass were determined. Plants were regenerated on MS medium supplemented with vitamins, antioxidants and 6-benzylaminopurine. At 20 weeks, morphological changes were observed with respect to the callus not treated with EMS. Using dried leaves, secondary metabolites were extracted and quantified by high performance liquid chromatography. Callus survival and growth decreased with increasing EMS concentration (mean lethal dose: 148.8 mM), in seedlings it generated changes in leaf morphology, dwarfism and the formation of three meristems, which would increase the number of leaves. Finally, 140 mM of EMS increased the content of caffeine, caffeic acid and chlorogenic acid.

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References

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Portada volumen XXV número 3

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Published

2023-09-01

How to Cite

Joya Dávila, J., Gutiérrez Miceli, F. A. ., Luján Hidalgo, M. C., Serrano Gómez, L. A. ., & Ruíz Sesma, B. (2023). Biochemical and morphometric changes in Coffea arabica after a treatment with ethyl methanosulfonate: Biochemical and morphometric changes in Coffea arabica. Biotecnia, 25(3), 36–41. https://doi.org/10.18633/biotecnia.v25i3.1969

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Vol. 25 No. 3 (2023): September - December

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Research Articles

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