Análisis genético y molecular de propiedades fisicoquímicas del almidón y su asociación con la modificación del endospermo en maíz de calidad proteínica

Autores/as

  • Elthon Vega-Álvarez
  • Karen Virginia Pineda-Hidalgo
  • Nancy Yareli Salazar-Salas
  • Omar Alejandro Soto-López
  • Vicente Adrián Canizalez-Román
  • José Antonio Garzón-Tiznado
  • Roberto Gutiérrez-Dorado
  • Jose A. Lopez-Valenzuela Universidad Autonoma de Sinaloa https://orcid.org/0000-0002-9358-5030

DOI:

https://doi.org/10.18633/biotecnia.v24i3.1725

Palabras clave:

Maíz de calidad proteínica, modificación del endospermo, almidón, expresión génica

Resumen

El maíz de calidad proteínica (QPM) fue creado convirtiendo el endospermo suave opaco-2 en un fenotipo vítreo, pero los mecanismos involucrados en esta modificación no se conocen por completo. Se utilizaron líneas recombinantes puras derivadas de las líneas K0326Y QPM y W64Ao2 para identificar loci de características cuantitativas (QTL) asociados con propiedades fisicoquímicas del almidón. También se usaron RILs contrastantes en vitrosidad para evaluar la expresión de genes de biosíntesis de almidón. El mapeo identificó 5 o 6 QTL para cada característica que explicaron en promedio el 66 % de la variación fenotípica. Tres de los QTLs en los bins 4.05, 5.04 y 9.03 se encontraron cerca de los genes de síntesis de almidón Brittle 2 (Bt2), Amylose extender 1 (Ae1), y Waxy 1 (Wx1), respectivamente. La expresión de Wx1 fue tres veces mayor en K0326Y QPM que en W64Ao2 y ocho veces mayor en líneas vítreas en comparación con las opacas, lo que correspondió con la mayor acumulación de la enzima almidón sintasa unida al gránulo I (GBSSI) y el mayor contenido de amilosa observado en las líneas vítreas. El incremento en la síntesis de amilosa podría resultar en gránulos de almidón con más regiones amorfas que favorecen su compactación. Estos resultados sugieren que la modificación del endospermo en QPM está asociada con la síntesis de almidón conteniendo más amilosa durante el desarrollo del grano, lo cual podría facilitar el empaquetamiento de los gránulos de almidón resultando en el fenotipo vítreo.

Descargas

Los datos de descargas todavía no están disponibles.

Citas

Churchill, G.A. and Doerge, R.W. 1994. Empirical threshold values for quantitative trait mapping. Genetics 138(3):963-971.

Denyer, K.a.y., Johnson, P., Zeeman, S. and Smith, A.M. 2001. The control of amylose synthesis. Journal of Plant Physiology 158:479-487.

Dombrink-Kurtzman, M.A. and Knutson, C.A. 1997. A study of maize endosperm hardness in relation to amylose content and susceptibility to damage. Cereal Chemistry 74(6):776-780.

Dong, Y., Zhang, Z., Shi, Q., Wang, Q., Zhou, Q. and Li, Y. 2015. QTL identification and meta-analysis for kernel composition traits across three generations in popcorn. Euphytica 204:649-660.

Gayral, M., Bakan, B., Dalgalarrondo, M., Elmorjani, K., Delluc, C., Brunet, S., Linossier, L., Morel, M.H. and Marion, D. 2015. Lipid partitioning in maize (Zea mays L.) endosperm highlights relationships among starch lipids, amylose, and vitreousness. Journal of Agricultural and Food Chemistry 63(13):3551-3558.

Gibbon, B.C., Wang, X. and Larkins, B.A. 2003. Altered starch structure is associated with endosperm modification in Quality Protein Maize. Proceedings of the National Academy of Sciences USA. 100(26):15329-15334.

Giroux, M.J. and Hannah, L.C. 1994. ADP-glucose pyrophosphorylase in shrunken-2 and brittle-2 mutants of maize. Molecular and General Genetics 243(4):400-408.

Grimaud, F., Rogniaux, H., James, M.G., Myers, A.M. and Planchot, V. 2008. Proteome and phosphoproteome analysis of starch granule-associated proteins from normal maize and mutants affected in starch biosynthesis. Journal of Experimental Botany 59(12):3395-3406.

Hennen-Bierwagen, T.A., Lin, Q., Grimaud, F., Planchot, V., Keeling, P.L., James, M.G. and Myers, A.M. 2009. Proteins from multiple metabolic pathways associate with starch biosynthetic enzymes in high molecular weight complexes: A model for regulation of carbon allocation in maize amyloplasts. Plant Physiology 149(3):1541.

Holding, D.R., Hunter, B.G., Chung, T., Gibbon, B.C., Ford, C.F., Bharti, A.K., Messing, J., Hamaker, B.R. and Larkins, B.A. 2008. Genetic analysis of opaque2 modifier loci in quality protein maize. Theoretical and Applied Genetics 117(2):157-170.

Holding, D.R., Hunter, B.G., Klingler, J.P., Wu, S., Guo, X., Gibbon, B.C., Wu, R., Schulze, J.M., Jung, R. and Larkins, B.A. 2011. Characterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbred. Theoretical and Applied Genetics 122(4):783-794.

Huang, B., Chen, J., Zhang, J., Liu, H., Tian, M., Gu, Y., Hu, Y., Li, Y., Liu, Y. and Huang, Y. 2011. Characterization of ADP-glucose pyrophosphorylase encoding genes in source and sink organs of maize. Plant Molecular Biology Reporter 29(3):563-572.

Jia, M., Wu, H., Clay, K.L., Jung, R., Larkins, B.A. and Gibbon, B.C. 2013. Identification and characterization of lysine-rich proteins and starch biosynthesis genes in the opaque2 mutant by transcriptional and proteomic analysis. BMC Plant Biology 13(1):60-74.

Juárez-García, E., Agama-Acevedo, E., Gómez-Montiel, N.O., Pando-Robles, V. and Bello-Pérez, L.A. 2013. Proteomic analysis of the enzymes involved in the starch biosynthesis of maize with different endosperm type and characterization of the starch. Journal of the Science of Food and Agriculture 93(11):2660-2668.

Konik-Rose, C.M., Moss, R., Rahman, S., Appels, R., Stoddard, F. and Master, G.M. 2001. Evaluation of the 40 mg swelling test for measuring starch functionality. Starch/Stärke 53:14–20.

Li, L., Blanco, M. and Jane, J. 2007. Physicochemical properties of endosperm and pericarp starches during maize development. Carbohydrate Polymers 67(4):630-639.

Li, N., Zhang, S., Zhao, Y., Li, B. and Zhang, J. 2011. Over-expression of AGPase genes enhances seed weight and starch content in transgenic maize. Planta 233(2):241-250.

Liu, H., Shi, J., Sun, C., Gong, H., Fan, X., Qiu, F., Huang, X., Feng, Q., Zheng, X., Yuan, N., Li, C., Zhang, Z., Deng, Y., Wang, J., Pan, G., Han, B., Lai, J. and Wu, Y. 2016. Gene duplication confers enhanced expression of 27-kDa γ-zein for endosperm modification in quality protein maize. Proceedings of the National Academy of Sciences USA. 113(18):4964-4969.

Livak, K.J. and Schmittgen, T.D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta c(T)) Method. Methods 25: 402-408.

Manly, K.F., Cudmore, R.H., Jr. and Meer, J.M. 2001. Map Manager QTX, cross-platform software for genetic mapping. Mammalian Genome 12(12):930-932.

Matveev, Y.I., Van Soest, J.J.G., Nieman, C., Wasserman, L.A., Protserov, V.A., Ezernitskaja, M. and Yuryev, V.P. 2001. The relationship between thermodynamic and structural properties of low and high amylose maize starches. Carbohydrate Polymers 44:151-160.

Mertz, E.T., Bates, L.S. and Nelson, O.E. 1964. Mutant gene that changes protein composition and increases lysine content of maize endosperm. Science 145:279-280.

Nakamura, Y., Umemoto, T., Ogata, N., Kuboki, Y., Yano, M. and Sasaki, T. 1996. Starch debranching enzyme (R-enzyme or pullulanase) from developing rice endosperm: purification, cDNA and chromosomal localization of the gene. Planta 199(2):209-218.

Nishi, A., Nakamura, Y., Tanaka, N. and Satoh, H. 2001. Biochemical and genetic analysis of the effects of amylose-extender mutation in rice endosperm. Plant Physiology 127(2):459-472.

Pereira, R., Davide, L., Pedrozo, C., Carneiro, N., Souza, I. and Paiva, E. 2008. Relationship between structural and biochemical characteristics and texture of corn grains. Genetics and Molecular Research 7( 2):498-508.

Salazar-Salas, N.Y., Pineda-Hidalgo, K.V., Chavez-Ontiveros, J., Gutierrez-Dorado, R., Reyes-Moreno, C., Bello-Pérez, L.A., Larkins, B.A. and Lopez-Valenzuela, J.A. 2014. Biochemical characterization of QTLs associated with endosperm modification in quality protein maize. Journal of Cereal Science 60(1):255-263.

Séne, M., Thévenot, C., Hoffmann, D., Bénétrix, F., Causse, M. and J.-L., P. 2001. QTLs for grain dry milling properties, composition and vitreousness in maize recombinant inbred lines. Theoretical and Applied Genetics 102:591–599.

Sethi, M., Singh, A., Kaur, H., Phagna, R.K., Rakshit, S. and Chaudhary, D.P. 2021. Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize. Scientific Reports 11(1):2469.

Tester, R.F. and Morrison, W.R. 1990. Swelling and gelatinization of cereal starches. I. Effects of amylopectin, amylose, and lipids. Cereal Chemistry 67:551-557.

Villegas, E., Vasal, S.K., Bjarnason, M. and Mertz, E.T. 1992. Quality protein maize-what is it and how was it developed. En: Quality Protein Maize. E.T. Mertz (Ed), p. 27-48. American Society of Cereal Chemists, Saint Paul, Minnesota.

Wallace, J., Lopes, M., Paiva, E. and Larkins, B.A. 1990. New methods for extraction and quantitation of zeins reveal a high content of γ-zein in modified opaque-2 maize. Plant Physiology 92:191-196.

Wu, Y., Holding, D.R. and Messing, J. 2010. γ-zeins are essential for endosperm modification in quality protein maize. Proceedings of the National Academy of Sciences USA. 107(29):12810-12815.

Zeng, Z.B. 1994. Precision mapping of quantitative trait loci. Genetics 136:1457-1468.

Zhang, J., Lu, X., Song, X., Yan, J., Song, T., Dai, J., Rocheford, T. and Li, J. 2008. Mapping quantitative trait loci for oil, starch, and protein concentrations in grain with high-oil maize by SSR markers. Euphytica 162:335-344.

Zhang, Z., Zheng, X., Yang, J., Messing, J. and Wu, Y. 2016. Maize endosperm-specific transcription factors O2 and PBF network the regulation of protein and starch synthesis. Proceedings of the National Academy of Sciences USA. 113(39):10842-10847.

Publicado

2022-10-17

Cómo citar

Vega-Álvarez, E., Pineda-Hidalgo, K. V., Salazar-Salas, N. Y., Soto-López, O. A., Canizalez-Román, V. A., Garzón-Tiznado, J. A., … Lopez-Valenzuela, J. A. (2022). Análisis genético y molecular de propiedades fisicoquímicas del almidón y su asociación con la modificación del endospermo en maíz de calidad proteínica. Biotecnia, 24(3), 140–149. https://doi.org/10.18633/biotecnia.v24i3.1725

Número

Sección

Artículos originales

Métrica

Artículos más leídos del mismo autor/a

Artículos similares

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 > >> 

También puede Iniciar una búsqueda de similitud avanzada para este artículo.