Detección del Virus de la Mancha Necrótica de Impatiens (INSV) en trips y plantas ornamentales mediante RT-LAMP
DOI:
https://doi.org/10.18633/biotecnia.v26.2256Palabras clave:
Amplificación Isotérmica mediada por bucle, RT-PCR, diagnóstico, virusResumen
El virus fitopatógeno INSV es transmitido por trips y es capaz de infectar un gran número de cultivos, causando enormes pérdidas tanto en hortalizas como en ornamentales. El objetivo de este trabajo fue mejorar la detección del virus INSV en diferentes especies de plantas ornamentales, por lo que se colectaron muestras tanto de trips como de plantas de Impatiens balsamina (balsamina), Impatiens hawkeri (belén), Catharanthus roseus (vinca), Cyclamen persicum (ciclamen) y Pelargonium hortorum (geranio) con síntomas de virosis y mediante la técnica de RT-PCR se identificaron muestras positivas que se usaron para estandarizar la técnica de RT-LAMP. Los resultados muestran que el empleo del RT-LAMP permite la detección del virus de una forma más simple y de 60 veces más sensible que el RT-PCR.
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