ACTIVIDADES BIOLÓGICAS DEL EXTRACTO METANÓLICO DE Rhodosorus marinus

María G. Burboa-Zazueta, Luis E. Gutiérrez-Millán, Miguel A. Valdéz-Covarrubias, Marco A. López-Torres, Armando Burgos-Hernández, Alfonso Garcia-Galaz

Resumen


Las microalgas marinas pueden ser una fuente de moléculas bioactivas; existen numerosos reportes de actividad antioxidante, antibacteriana y antiproliferativa de extractos obtenidos a partir de macroalgas. El objetivo del presente trabajo fue evaluar la actividad citotóxica, antioxidante y antimutagénica del extracto metanólico de la microalga roja Rhodosorus marinus. El extracto fue obtenido a partir de biomasa liofilizada mediante lisis ácida y sonicación. Se evaluó la actividad citotóxica frente a 7 líneas celulares humanas con el ensayo MTT, la actividad antioxidante por ABTS y DPPH y la actividad antimutagénica con las cepas TA98 y TA100 del ensayo de Ames. Se encontró actividad citotóxica frente a 5 de las 7 líneas evaluadas. Los porcentajes de inhibición para la actividad antioxidante fueron de 25.60 + 4.03% (DPPH) y 5.59 + 0.63% (ABTS). Para el ensayo de Ames, frente a ambasm cepas probadas se alcanzaron porcentajes de inhibición de colonias revertantes de aproximadamente el 75% en la concentración más alta evaluada, lo cual indica una fuerte actividad antimutagénica. Los resultados mostraron actividad biológica en las diferentes pruebas realizadas, por lo que se infiere que el extracto metanólico contiene moléculas bioactivas de importancia en la salud y para diferentes usos biotecnológicos.


Palabras clave


Citotóxica; antimutagénica; Rhodosorus marinus

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Referencias


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DOI: http://dx.doi.org/10.18633/biotecnia.v20i3.711

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