Identification of rhizobacteria isolated from Tagetes coronopifolia and Tagetes terniflora (Cempasúchitl) and evaluation of their inhibitory capacity in vitro of phytopathogenic microorganisms
DOI:
https://doi.org/10.18633/biotecnia.v24i3.1736Keywords:
rhizobacteria, endophytic bacteria, biological control, antagonistic plantsAbstract
Tagetes is a group of plants commonly known as cempasúchil or “flower of the dead”, which are mainly recognized for their use in the food industry, ornamental horticulture, therapeutic herbalism and culture. Tagetes coronopifolia and terniflora are two species plants of this genus, which are used in crop rotation to control phytopathogenic nematodes. In the curent work, 581 strains were isolated from samples of rhizosphere soil, soil attached to the root and the interior of the root at 30, 60, 90 and 120 days of growth, in greenhouse cultures. From the total isolated rhizobacteria strains, 22 presented antagonistic activity to Fusarium sp. and 53 to Stenocarpella maydis, both. pathogenic fungi in corn, while 37 strains showed antagonism to the pathogenic bacteria Clavibacter michiganensis subsp michiganensis. According to the percentage of inhibition of the phytopathogens, 10 rhizobacteria were selected for molecular identification using a fragment of approximately 1484 bp of the 16SrDNA gene. The similarity analysis using the NCBI BLAST tool, showed that the rhizobacteria belong to the genera Bacillus and Pseudomonas, which are used as biocontrol agents.
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