Biological effectiveness of extracts of Agave striata and Fouquieria splendens against Clavibacter michiganensis subp. michiganensis.

Authors

  • Jesús Eduardo Ramírez Méndez Antonio Narro Agrarian Autonomous University. Department of Parasitology. Antonio Narro Road 1923, Buenavista. ZIP. 25315. Saltillo, Coahuila, Mexico. https://orcid.org/0000-0001-7044-3972
  • Francisco Daniel Hernández Castillo Universidad Autónoma Agraria Antonio Narro
  • Marco Antonio Tucuch Pérez Antonio Narro Agrarian Autonomous University. Department of Parasitology. Antonio Narro Road 1923, Buenavista. ZIP. 25315. Saltillo, Coahuila, Mexico. https://orcid.org/0000-0002-6978-8145
  • Isaac Irving Camacho Aguilar Antonio Narro Agrarian Autonomous University. Department of Parasitology. Antonio Narro Road 1923, Buenavista. ZIP. 25315. Saltillo, Coahuila, Mexico. https://orcid.org/0000-0002-5929-1506
  • Roberto Arredondo Valdés Autonomous University of Coahuila Faculty of Chemical Sciences Department of Nanobiosciences. Saltillo, Coahuila, Mexico. Ing. J.Cardenas Valdez S/N, Col. República, CP. 25280. Saltillo, Coahuila, Mexico. https://orcid.org/0000-0003-4093-0707
  • José Ángel Villarreal Quintanilla Antonio Narro Agrarian Autonomous University. Department of Botany. Antonio Narro Road 1923, Buenavista. ZIP. 25315. Saltillo, Coahuila, Mexico.

DOI:

https://doi.org/10.18633/biotecnia.v25i1.1751

Keywords:

Clavibacter michiganensis Subsp. michiganensis, tomato, methanolic extracts, biological effectiveness.

Abstract

Bacterial canker is one of the tomatoes (Solanum lycopersicum) diseases caused by the bacterium Clavibacter michiganensis subsp. michiganensis (Cmm). In this study, Cmm was identified by end-point PCR using the primers CMM5F and CMM6R. The efficacy of methanolic extracts of Agave striata and Fouquieria splendens on Cmm in vitro under greenhouse conditions was evaluated, as well as information about the composition of phytochemicals present in plants. The in vitro assay was performed using the plate microdilution technique from 3.9 mg/L to 1000 mg/L, the Ci50 and Ci90 of each extract was determined. In the greenhouse experiment, the effect of the extracts and an organic product "Biobacter" against Cmm was evaluated on incidence, severity, and morphometric parameters. The in vitro results indicated that the extracts of F. splendens and A. striata showed inhibition on the phytopathogen. The greenhouse trial showed that the incidence, severity and morphometric parameters were lower with F. splendens extract, subsequently, with Biobacter product.

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Author Biographies

Jesús Eduardo Ramírez Méndez, Antonio Narro Agrarian Autonomous University. Department of Parasitology. Antonio Narro Road 1923, Buenavista. ZIP. 25315. Saltillo, Coahuila, Mexico.

Biomonitoring of heavy metals in tree vegetation in the city of Saltillo.

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Biological Efficacy of Trichoderma spp. and Bacillus spp. in the Management of Plant Diseases.

Antifungal activity of phytochemical compounds of extracts from Mexican semi-desert plants against Fusarium oxysporum from tomato by microdilution in plate method.

 

Isaac Irving Camacho Aguilar, Antonio Narro Agrarian Autonomous University. Department of Parasitology. Antonio Narro Road 1923, Buenavista. ZIP. 25315. Saltillo, Coahuila, Mexico.

Hosts and Vectors of Xylella fastidiosa in Vineyards of Parras, Coahuila, Mexico.

Roberto Arredondo Valdés, Autonomous University of Coahuila Faculty of Chemical Sciences Department of Nanobiosciences. Saltillo, Coahuila, Mexico. Ing. J.Cardenas Valdez S/N, Col. República, CP. 25280. Saltillo, Coahuila, Mexico.

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José Ángel Villarreal Quintanilla, Antonio Narro Agrarian Autonomous University. Department of Botany. Antonio Narro Road 1923, Buenavista. ZIP. 25315. Saltillo, Coahuila, Mexico.

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2022-11-25

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Ramírez Méndez, J. E. ., Hernández Castillo, F. D., Tucuch Pérez, M. A., Camacho Aguilar, I. I. ., Arredondo Valdés, R. ., & Villarreal Quintanilla, J. Ángel . (2022). Biological effectiveness of extracts of Agave striata and Fouquieria splendens against Clavibacter michiganensis subp. michiganensis. Biotecnia, 25(1), 34–42. https://doi.org/10.18633/biotecnia.v25i1.1751

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Vol. 25 No. 1 (2023): January - April

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