Stages of the cryopreservation protocol and its effect on the viability and regeneration of grape-vine zygotic embryos (Vitis vinifera L.)
DOI:
https://doi.org/10.18633/biotecnia.v25i3.1991Keywords:
Key words: cryoprotectant, liquid nitrogen, vitrificationAbstract
The grapevine is one of the main crops in the world and the state of Sonora is the largest producer in Mexico. This crop is in constant danger due to various biotic and abiotic factors, therefore the importance of its conservation. Cryopreservation is ideal for this culture, but it can cause physiological, molecular, and biochemical alterations, affecting viability and regeneration. Hence, in this work it was proposed to analyze the effect of the stages of the cryopreservation protocol on the viability and regeneration of grapevine zygotic embryos. Tissue viability (V) and seedling regeneration (RP) showed that PVS2 protects embryos (V:85%, PR:60%), while combined exposure to PVS2 solution and liquid nitrogen (NL) caused decreased viability and regeneration (V: 68%; PR: 2%). A more drastic effect was observed when the tissue was exposed to PVS2+NL and rewarming (RC) (V:68%; PR:0%). However, viability and regeneration recovered when the tissue was subjected to PVS2+NL+RC and unloading solution (SD) (V:92%; RP:60%). It is concluded that the use of the unloading solution is essential to avoid tissue damage due to the different stages of cryopreservation.
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