Efecto dosis-dependiente de la CHH-B1 recombinante sobre la capacidad osmorreguladora y la expresión de la Na+/K+-ATPasa en camarones Litopenaeus vannamei sometidos a ablacion bilateral del pedúnculo ocular
DOI:
https://doi.org/10.18633/biotecnia.v23i3.1441Keywords:
Litopenaeus vannamei, camarón, hormona hiperglucémica de crustáceos, capacidad osmoreguladora, a /K -ATPasaAbstract
Osmoregulation in crustaceans is under neuroendocrine control by the crustacean hyperglycemic hormones (CHHs), which modify water and ion concentrations in diverse species. Previous studies done by our group suggested that CHH variant B1 (CHH-B1) has effects on the osmoregulatory responses of the Pacific white shrimp Litopenaeus vannamei. For a better understanding of the molecular action mechanisms of CHH-B1 in osmoregulation, in this work, we established the dose-dependent effect of recombinant CHH-B1 on the gene expression of Na+/K+-ATPase (NKA) in gills, in contrast to changes in the osmoregulatory capacity (OC) of bilaterally eyestalk-ablated shrimp under hyper-osmotic conditions. The results indicate that CHH-B1 regulates the OC of shrimp during hypo-regulation by modulating Na+/K+-ATPase at a transcriptional level. Our results suggest that CHH has a direct participation in the control of osmo-ionic regulation mechanisms, not only in L. vannamei but in crustaceans in general.
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REFERENCES
Camacho-Jiménez, L., Sánchez-Castrejón, E., Ponce-Rivas, E., Muñoz-Márquez. M.E., Aguilar, M.B., Re, A.D. y Díaz, F. 2015. Hyperglycemic activity of the recombinant crustacean hyperglycemic hormone B1 variant (CHH-B1) of the Pacific white shrimp Litopenaeus vannamei. Peptides. 71: 32-39.
Camacho-Jiménez, L., Díaz, F., Muñoz-Márquez, M.E, Farfán, C., Re, A.D. y Ponce-Rivas, E. 2017a. Hyperglycemic and osmotic effects of dopamine and recombinant hormone CHH- B1 in the Pacific white shrimp Litopenaeus vannamei. Marine and Freshwater Behaviour and Physiology. 50: 67-79.
Camacho-Jiménez, L., Sánchez-Castrejón E., Díaz, F., Aguilar, M.B., Muñoz-Márquez, M.E. y Ponce-Rivas, E. 2017b. Cloning and expression of the recombinant crustacean hyperglycemic hormone variant B2 (rCHH-B2) and its effects on the metabolism and osmoregulation of the Pacific white shrimp Litopenaeus vannamei. General and Comparative Endocrinology. 253: 33-43.
Camacho-Jiménez, L., Díaz, F., Sánchez-Castrejón, E. y Ponce-Rivas, E. 2018. Effects of the recombinant crustacean hyperglycemic hormones rCHH-B1 and rCHH-B2 on the osmo-ionic regulation of the shrimp Litopenaeus vannamei exposed to acute salinity stress. Journal of Comparative Physiology B. 188: 565-579.
Castille, F.L. y Lawrence A.L. 1981. The effect of salinity on the osmotic, sodium and chloride concentrations in the hemolymph of euryhaline shrimp of the genus Penaeus. Comparative Biochemistry and Physiology Part A. 68: 75-80.
Chang, E.S. 2005. Stressed-out lobsters: crustacean hyperglycemic hormone and stress proteins. Integrative and Comparative Biology. 45: 43-50.
Chapelle, S y Zwingelstein G. 1984. Phospholipid composition and metabolism of crustacean gills as related to changes in environmental salinities: relationship between Na+-K+-ATPase activity and phospholipids. Comparative Biochemistry and Physiology Part B. 78: 363-372.
Charmantier, G., Charmantier-Daures, M. y Aiken, D.E. 1984. Neuroendocrine control of hydromineral regulation in the american lobster Homarus americanus H. Milne-Edwards 1837 (Crustacea, Decapoda) 1. Juveniles. General and Comparative Endocrinology. 54: 8-19.
Charmantier-Daures, M., Charmantier, G, Janssen, K.P.C., Aiken, D.E. yVan Herp, F. 1994. Involvement of eyestalk factors in the neuroendocrine control of osmoregulation in adult american lobster Homarus americanus. General and Comparative Endocrinology. 94: 281-293.
Charmantier, G., Charmantier-Daures, M. y Towle, D 2009. Osmotic and ionic regulation in aquatic arthropods. En: Osmotic and Ionic Regulation: cell and animals. D.H. Evans (ed), pp. 165-230. Taylor & Francis Group, Boca Raton.
Chong-Robles, J., Charmantier, G., Boulo, V., Lizarraga-Valdéz, J., Enríquez-Paredes, L.M. y Giffard-Mena I. 2014. Osmoregulation pattern and salinity tolerance of the white shrimp Litopenaeus vannamei (Boone, 1931) during post-embryonic development. Aquaculture. 422-423: 261-267.
Chung, J.S. y Webster, S.G. 2006. Binding sites of crustacean hyperglycemic hormone and its second messengers on gills and hindgut of the green shore crab, Carcinus maenas: a possible osmoregulatory role. General and Comparative Endocrinology. 147: 206-213.
Chung, J.S., Zmora, N., Katayama, H. y Tsuitsui, N. 2010. Crustacean hyperglycemic hormone (CHH) neuropeptides family: functions, titer, and binding to target tissues. General and Comparative Endocrinology.166: 447-454.
Díaz, F., Farfán, C., Sierra, E. y Re, A.D. 2001. Effects of temperature and salinity fluctuation on the ammonium excretion and osmoregulation of juveniles of Penaeus vannamei. Marine and Freshwater Behaviour and Physiology. 34: 93-104.
Evans, D.H. Evans, D. H., Piermarini, P. M. y Choe, K. P. 2005. The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid–base regulation, and excretion of nitrogenous waste. Physiological Reviews. 85: 97-177.
Fanjul-Moles, M.L. 2006. Biochemical and functional aspects of crustacean hyperglycemic hormone in decapod crustaceans: review and update. Comparative Biochemistry and Physiology Part C. 142: 390-400.
Fawcett, T.W., Martindale, J.L, Guyton, K.Z., Hai, T. y Holbrook, N.J. 1999. Complexes containing activating transcription factor (ATF)/cAMP-responsive element-binding protein (CREB) interact with the CCAAT/enhancer-binding protein (C/EBP)–ATF composite site to regulate Gadd153 expression during the stress response. Biochemical Journal. 339: 135-141.
Henry, R.P., Lucu, Č., Onken, H. y Weihrauch, D. 2012. Multiple functions of the crustacean gill: osmotic/ionic regulation, acid-base balance, ammonia excretion, and bioaccumulation of toxic metals. Frontiers in Physiology. 3: 1–33.
Holthius, L.B. 1980. FAO species catalogue. Vol.1. Shrimps and prawns of the world. FAO Fisheries Synopsis no. 125.
Jayasundara, N. Towle, D.W., Weihrauch, D. y Spanings-Pierrot, C. 2007. Gill-specific transcriptional regulation of Na+/K+-ATPase α-subunit in the euryhaline shore crab Pachygrapsus marmoratus: sequence variants and promoter structure. Journal of Experimental Biology. 210: 2070-2081.
Kamemoto, F.I. 1976. Neuroendocrinology of osmoregulation in decapod Crustacea. American Zoologist. 16: 141-150.
Katayama, H. y Chung, J.S. 2009. The specific binding sites of eyestalk- and pericardial organ-crustacean hyperglycaemic (CHHs) in multiples tissues of the blue crab, Callinectes sapidus. Journal of Experimental Biology. 212: 42-49.
Lago-Lestón, A., Ponce, E. y Muñoz-Márquez, M.E. 2007. Cloning and expression of hyperglycemic (CHH) and molt-inhibiting (MIH) hormones mRNAs from the eyestalk of shrimps of Litopenaeus vannamei grown in different temperature and salinity conditions. Aquaculture. 270: 343-357.
Lignot, J.H., Trilles, J.P. y Charmantier, G. 1997. Effect of an organophosphorus insecticide, fenitrothion, on survival and osmoregulation of various developmental stages of the shrimp Penaeus japonicus (Crustacea: Decapoda). Marine Biology. 128: 307-316.
Li, E., Arena, L., Chen, L., Quin, J.G. y Van Wormhoudt, A. 2009. Characterization and Tissue-Specific Expression of the two glutamate dehydrogenase cDNAs in Pacific white shrimp, Litopenaeus vannamei. Journal of Crustacean Biology. 29: 379-386.
Liu, H.Y., Pan, L.Q. y Zheng, D.B. 2008. Injection of biogenic amines modulates osmoregulation of Litopenaeus vannamei: response of hemolymph osmotic pressure, ion concentration and osmolality effectors. Comparative Biochemistry and Physiology Part A. 151: 191-197.
Liu, H.Y., Pan, L.Q. y Zheng, D.B. 2009. Effects of injection of biogenic amines on expression of gill related ion transporter mRNA and α-subunit protein in Litopenaeus vannamei. Comparative Biochemistry and Physiology Part A. 154: 29-36.
Liu, M., Pan, L., Li, L. y Zheng D. 2014. Molecular cloning, characterization and recombinant expression of crustacean hyperglycemic hormone in white shrimp Litopenaeus vannamei. Peptides. 53: 115-124.
Liu, C.J., Huang, S.S., Toullec, J.Y., Chang, C.Y., Chen, Y.R., Huang, W.S. y Lee, C.Y. 2015. Functional assessment of residues in the amino-and carboxyl-termini of crustacean hyperglycemic hormone (CHH) in the mud crab Scylla olivacea using point-mutated peptides. PLoS One. 10:e0134983.
Lorenzon, S. 2005. Hyperglycemic stress response in crustaceans. Invertebrate Survival Journal. 2: 131-141.
Lucu, Č. y Towle, D.W. 2003. Na+ + K+-ATPase gills of aquatic crustacea. Comparative Biochemistry and Physiology Part A. 135: 195-214.
Mantel, L.H. 1985. Neurohormonal integration of osmotic and ionic regulation. American Zoologist. 25: 253-263.
Montiel-Arzate, A., Sánchez-Castrejón, E., Camacho-Jiménez, L., Díaz, F. y Ponce-Rivas, E. 2020. Effect of recombinant crustacean hyperglycemic hormones rCHH-B1 and rCHH-B2 on lipid metabolism in the Pacific white shrimp Litopenaeus vannamei. Aquaculture Research. 51: 4267-4278.
Mosco, A., Zlatev, S., Guarnaccia, C. y Giulanini, P.G. 2015. Functional analysis of a mutated analogue of the crustacean hyperglycemic hormone from the crayfish Pontastacus leptodactylus. Journal of Experimental Zoology Part A. 323: 121-127.
Péqueux, A. 1995. Osmotic regulation in crustaceans. Journal of Crustacean Biology. 15: 1-60.
Sainz-Hernández, J.C., Racotta, I.S., Dumas, S. y Hernández-López, J. 2008. Effect of unilateral and bilateral eyestalk ablation in Litopenaeus vannamei male and female on several metabolic and immunologic variables. Aquaculture. 283: 188-193.
Sánchez-Castrejón, E., Ponce-Rivas, E., Aguilar, M.B. y Díaz, F. 2008. Molecular cloning and expression of a putative crustacean hyperglycemic hormone of Litopenaeus vannamei in Pichia pastoris. Electronic Journal of Biotechnology. 11: 9-10.
Sathapondecha, P., Panyim, S. y Udomkit, A. 2014. Molecular characterization of a cDNA encoding red pigment-concentrating hormone in black tiger shrimp Penaeus monodon: implication of its function in molt and osmoregulation. Comparative Biochemistry and Physiology Part. A. 175: 124–130.
Serrano, L., Blanvillain, G., Soyez, D., Charmantier, G., Grousset, E., Aujulat, G. y Spanings-Pierrot, C. 2003. Putative involvement of crustacean hyperglycemic hormone isoforms in the neuroendocrine mediation of osmoregulation in the crayfish Astacus leptodactylus. Journal of Experimental Biology. 206: 979-988.
Spanings-Pierrot, C., Soyez, D., Van Herp, F., Gompel, M., Skaret, G., Grousset, E. y Charmantier, G. 2000. Involvement of crustacean hyperglycemic hormone in the control of gill ion transport in the crab Pachygrapsus marmoratus. General and Comparative Endocrinology.119: 340-350.
Sun, H., Zhang, L., Ren, C., Chen, C., Fan, S., Xia, J. J., Lin, H. y Hu, C. 2011. The expression of Na, K-ATPase in Litopenaeus vannamei under salinity stress. Marine Biology Research.7: 623-628.
Tiu, S.H., He J.G. y Chan, S.M. 2007. The LvCHH-ITP gene of the shrimp (Litopenaeus vannamei) produces a widely expressed putative ion transport peptide (LvITP) for osmo-regulation. Gene. 396: 226-235.
Ventura-López, C., Gómez-Anduro, G., Arcos, F.G, Llera-Herrera, R., Racotta, I.S. y Ibarra, A.M. 2016. A novel CHH gene from the Pacific white shrimp Litopenaeus vannamei was characterized and found highly expressed in gut and less in eyestalk and other extra-eyestalk tissues. Gene. 582: 148-160.
Webster, S.G., Keller, R. y Dircksen, H. 2012. The CHH-superfamily of multifunctional peptide hormones controlling crustacean metabolism, osmoregulation, moulting, and reproduction. General and. Comparative Endocrinology. 175: 217-233.
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