Pinus greggii inoculation with the ectomycorrhizal fungi Laccaria laccata enhance native population of plant-growth-promoting rhizobaceria
DOI:
https://doi.org/10.18633/biotecnia.v23i2.1302Keywords:
Pines, Biolog EcoPlates, icrobial diversity, phosphate solubilization, nitrogen fixation.Abstract
The pine-ectomicorrhiza symbiosis is of great ecological importance due to the microbial interaction established in mycorrhizosphere and the favorable edaphic conditions created by the decomposition of organic matter. The objective of this work was to estimate the effect of the colonization of the ectomycorrhizal fungus Laccaria laccata on the growth of Pinus greggii, as well as the abundance of microorganisms in its roots, under greenhouse conditions. It was observed that one year after sowing and 6 months after inoculation, L. laccata positively influenced the growth of P. greggii; in addition, the microbial metabolic activity (AWCD) increased, but there were no differences in the metabolic diversity index with respect to the control. In turn, a greater abundance of phosphate-solubilizing and nitrogen-fixing bacteria was found in the rhizosphere of the inoculated plants. This suggests that these organisms are favored under the conditions that induced the presence of L. laccatta.
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References
Berruti A, E Lumini, R Balestrini V Bianciotto. 2016. Arbuscular mycorrhizal fungi as natural biofertilizers: Let’s benefit from past successes. Frontiers in Microbiology 6:1559-103389.
Bolívar-Anillo HJ, ML Contreras-Zentella and LG Teherán-Sierra. 2016. Burkholderia tropica una bacteria con gran potencial para su uso en la agricultura. Tip 19: 102-108.
Garland J.L. y Mills A.L. 1991. Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization. Appl. Environ. Microbiol. 57: 2351-2359.
Ge Z, H Du, Y Gao and W Qiu. 2018. Analysis on metabolic functions of stored rice microbial communities by BIOLOG ECO microplates. Frontiers in Microbiology 9: 1375.
Hassani MA, P Durán, S Hacquard. 2018. Microbial interactions within the plant holobiont. Microbiome 6: 1-16.
Hoeksema JD, VB Chaudhary, CA Gehring, NC Johnson, J Karst, RT Koide, A Pringle, C Zabinski, JD Bever, JC Moore. 2010. A meta-analysis of context-dependency in plant response to inoculation with mycorrhizal fungi. Ecology Letters 13:394-407.
Kuppardt A, T Fester, C Härtig and A Chatzinotas. 2018. Rhizosphere Protists Change Metabolite Profiles in Zea mays. Frontiers microbiology 9: 169-178.
Li K., MJ DiLegge, IS Minas, A Hamm, D Manter and JM Vivanco. 2019. Soil sterilization leads to re-colonization of a healthier rhizosphere microbiome. Rhizosphere 12: 100176.
Marupakula S, S Mahmood, J Jernberg, S Nallanchakravarthula, ZA Fahad and RD Finlay. 2017. Bacterial microbiomes of individual ectomycorrhizal Pinus sylvestris roots are shaped by soil horizon and differentially sensitive to nitrogen addition. Environmental Microbiology 19: 4736-4753.
Rentería-Chávez MC, J Pérez-Moreno, VM Cetina-Alcalá, R Ferrera-Cerrato y B Xoconostle-Cázares. 2017. Transferencia de nutrientes y crecimiento de Pinus greggii Engelm. inoculado con hongos comestibles ectomicorrícicos en dos sustratos. Revista Argentina de Microbiología 49: 93-104.
Setälä H. 2000. Reciprocal interactions between Scots pine and soil food web structure in the presence and absence of ectomycorrhiza. Oecologia 125: 109-118.
Tian J, J Wang, M Dippold, Y Gao, E Blagodatskaya, and Y Kuzyakov. 2016. Biochar affects soil organic matter cycling and microbial functions but does not alter microbial community structure in a paddy soil. Science of the Total Environment 556: 89-97.
Vandenkoornhuyse P, A Quaiser, M Duhamel, A Le Van, A Dufresne. 2015. The importance of the microbiome of the plant holobiont. New Phytologist 206: 1196-1206.
Viollet A, B Pivato, C Mougel, JC Cleyet-Marel, C Gubry-Rangin, P Lemanceau and S Mazurier. 2017. Pseudomonas fluorescens C7R12 type III secretion system impacts mycorrhization of Medicago truncatula and associated microbial communities. Mycorrhiza 27: 23-33.
Wang S, T Li, Z Zheng and HY Chen. 2019. Soil aggregate-associated bacterial metabolic activity and community structure in different aged tea plantations. Science of The Total Environment 654: 1023-1032.
Zheng W, EK Morris and MC Rillig. 2014. Ectomycorrhizal fungi in association with Pinus sylvestris seedlings promote soil aggregation and soil water repellency. Soil Biology and Biochemistry 78: 326-331.
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