un Modeling radial growth of Amylomyces rouxii and its tolerance to selected pharmaceutical active compounds
DOI:
https://doi.org/10.18633/biotecnia.v26.2292Keywords:
fungi, inhibition growth, logistic equation, microcontaminants, growth modelingAbstract
Filamentous fungi that tolerate the presence of pharmaceutical active compounds (PhACs) have the potential application of removing them. In this work it was showed that radial growth data can be modeled and used to determine the kinetic parameters to quantify fungal growth in the presence of PhACs. The capacity of Amylomyces rouxii to grow in the presence of 12 PhACs at concentrations between 100 to 5000 µg L-1 was evaluated. The studied PhCAS were paracetamol, ibuprofen, diclofenac, naproxen, sulfamethoxazole, trimethoprim, ciprofloxacin, ofloxacin, carbamazepine, β-estradiol, triclosan, and bisphenol-A. The data of A. rouxii radial growth was modeled using the logistic equation and linear regression. The Vmax, except in cultures with βETD, and µmax values were not affected by the presence of PhACs. Growth inhibition of fungus was calculated at 24 hours. In cultures with diclofenac, triclosan and naproxen, a linear relationship was observed between concentration and radial growth inhibition. However, there was no difference in radial growth inhibition at the different assayed concentrations of ibuprofen, trimethoprim, and β-estradiol. In culture with 5000 µg carbamazepine L-1, growth of A. rouxii was completely inhibited. To the best of our knowledge, this is one the first work reporting PhACs toxicity in zygomycetes.
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