Biostimulant effect of Macrocystis pyrifera on Metarhizium anisopliae spores and its pathogenicity against Chloridea virescens
DOI:
https://doi.org/10.18633/biotecnia.v26.2321Keywords:
Biotechnology, microorganisms, sustainable developmentAbstract
The biostimulant effect of Macrocystis pyrifera on Metarhizium anisopliae spores was evaluated in its growth, sporulation, viability, and pathogenicity against Chloridea virescens. Nine concentrations were prepared at 1×107,8,9 spores/ml with 5 %, 10 % and 20 % algae in SDA plus three three positive check, and were incubated at 150 r.p.m, 28 oC, 80 % relative humidity and 8 h of light. Pathogenicity was tested on larvae two days after hatching. M. pyrifera had a biostimulant effect on M. anisopliae, and improved with 5 % and 10 % with growth of 4.91 µm/d, and with 20 % 4.84 µm/d, viability was around 99 % germinated spores at 24 h in whole treatments, the total sporulation was 6 × 106 to 6.7 × 108, and with only fungus from 3 ×106 to 3.4 × 107 spores/ml. The highest mortality was 91.5 ± 2.5 % at 43.3 ± 1.3 h and with only fungus it was 80 ± 3 % at 70 ± 5 h. This work is a contribution to pest control with entomopathogenic fungi improved with extracts of marine origin. It is recommended to evaluate these treatments in conventional crop.
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