Expresión y tropismo del Herpesvirus de Ostreidos tipo 1 en dos tejidos del ostión del Pacífico Crassostrea gigas
DOI:
https://doi.org/10.18633/biotecnia.v21i3.1009Keywords:
Herpesvirus de ostreidos-1, OsHV-1, Tropismo, ExpresiónAbstract
El Herpesvirus de Ostreidos-1 (OsHV-1), causa altas mortalidades en los cultivos de Crassostrea gigas, este ha sido estudiado ampliamente para poder entender su patogénesis, sin embargo, no se ha estudiado donde prefiere replicar sus genes y donde estar como un virus activo. El objetivo del presente trabajo fue evaluar la expresión (ARNm) y el gen (ADNg) de la ADN polimerasa viral en dos tejidos de Crassostrea gigas infectado experimentalmente. Se realizó un bioensayo de infección y se tomaron muestras de tejidos para extraer ADN y ARN, a diferentes horas post infección. Los cambios en la expresión del gen de la ADN polimerasa se evaluaron por qPCR y por histología se detectaron los cambios en la estructura de los tejidos. Los resultados indican que el OsHV-1 replica su ADN en células del tejido de branquia, ya que se detectó el mensaje de la ADN polimerasa 59 veces más que el gen. Mientras que en las células de manto se cuantificó el gen 31 veces más que la expresión del mensaje del ORF100. Lo que sugiere un tropismo del OsHV-1, primero por las células del tejido de branquias en las cuales replica su ADN y seguido de las células del tejido de manto en el cual se encuentra como virus activo.Downloads
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