IDENTIFICACIÓN DE LAS PROTEÍNAS INTEGRALES DE MEMBRANA CONSIDERADAS FACTORES DE PATOGENICIDAD EN LA BACTERIA INTRACELULAR Candidatus Hepatobacter penaei MEDIANTE ANÁLISIS BIOINFORMÁTICO

Jesús Alberto Pérez-Acosta, Luis Rafael Rafael Martínez-Córdova, Teresa Gollas-Galván, José Ángel Huerta-Ocampo, Luis Enrique Gutiérrez-Millán, Marco Antonio López-Torres, Juan Manuel Leyva, Marcel Martínez-Porchas

Resumen


Candidatus Hepatobacter penaei se clasifica como una bacteria intracelular, Gram negativa y pleomórfica. La enfermedad que causa en camarón de cultivo representa una importante amenaza para la actividad camaronícola, por lo que se requiere conocer su mecanismo de infección. El objetivo de este trabajo fue identificar mediante análisis bioinformático, las proteínas membranales de Candidatus Hepatobacter penaei homólogas de los factores de patogenicidad de organismos tipo rickettsia (OTR). Para ello se realizó la predicción de genes codificantes de proteínas, utilizando el servidor público GeneMark. Para la identificación de las proteínas integrales de membrana que son factores de patogenicidad en la cepa de Candidatus Hepatobacter penaei, se empleó el software Blast2GO. Los resultados obtenidos muestran 96 proteínas integrales de membrana, reconocidas en la literatura como factores de patogenicidad bacteriana, con valores promedio de identidad mayor de 90 %. La identificación de los componentes de virulencia de Candidatus Hepatobacter penaei representa información relevante para entender en profundidad su mecanismo de infección.


Palabras clave


Candidatus Hepatobacter penaei; proteína; patogenicidad; OTR; GeneMark; Blast2GO

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Referencias


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DOI: http://dx.doi.org/10.18633/biotecnia.v20i3.719

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