Characterization and selection of native yeast isolated from natural fermentation for the production of the artisanal beverage bacanora
DOI:
https://doi.org/10.18633/biotecnia.v23i1.1221Keywords:
Bacanora, alcoholic fermentation, native yeast, S. cerevisiae, non-SaccharomycesAbstract
Bacanora is a Mexican distilled beverage made from agave, similar to tequila and mescal, produced with an artisanal process. Therefore the organoleptic characteristics of different bacanora vary highly. The aim of this study was to characterize and select native yeast involved in this artisanal process of fermentation. An initial pre-selection based upon the phenotypic characteristics of 580 strains of yeast was done, and then with fermentation characteristics with agave juice, to select some strains which satisfy with desirable. The Saccharomyces cerevisiae yeasts produced at the end of the fermentation 8.27 ± 0.24 g/L of biomass, 25.51 ± 1.19 g/L of ethanol, with a volumetric productivity of ethanol of 1.76±0.17 g/L/h. While for the three strains non-Saccharomyces the parameters were 3.86 ± 1.14 g/L, 15.56± 1.68 g/L and 0.484±0.06 g/L/h respectively. In the mixed culture of three S. cerevisiae, all remained at the end, but one of them predominates and of non-Saccharomyces yeasts, Torulaspora delbrueckii was the predominant yeast, this according with PFGE results. The results showed that the native yeasts are adapted to the agave juice used for production of bacanora and these yeasts would contribute to improve the ethanol yields and the organoleptic characteristics of Bacanora.
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Aguirre-Rivera, J., Aldrete-Herrera, P., Alvarez-Ainza, M. et al. 2017. Panorama del aprovechamiento de los agaves en México. Estado del Arte publicado por AGARED, Red Temática Mexicana Aprovechamiento Integral Sustentable y Biotecnología de los Agaves. ISBN: 978-607-97548-5-3. [consultado 07 febrero de 2020]. Available from: http:// www.agared.org/libro-panorama-de-los-agaves.
Alvarez-Ainza, M., González-Ríos, H., González-León, A., Valenzuela-Quintanar, A. and Acedo-Félix, E. 2013. Quantification of major volatile compounds in artisanal Bacanora. American Journal of Analytical Chemistry. 4: 683- 688.
Alvarez-Ainza, M., Zamora-Quiñonez, K. and Acedo-Félix, E. 2009. Perspectivas para el uso de levaduras nativas durante la elaboración de bacanora. Revista Latinoamericana de Microbiología. 51: 58-63.
Alvarez-Ainza, M., Zamora-Quiñonez, K., Moreno-Ibarra, G. and Acedo-Felix, E. 2015. Genomic diversity of Saccharomyces cerevisiae yeast associated with alcoholic fermentation of bacanora produced by artisanal methods. Applied Biochemestry and Biotechnology. 175: 2668-2676.
Arellano, M., Pelayo, C., Ramírez, J. and Rodríguez, I. 2008. Characterization of kinetic parameters and the formation of volatile compounds during the tequila fermentation by wild yeast isolated from agave juice. Journal of Industrial Microbiology and Biotechnology. 35: 835-841.
Bely, M., Stoeckle, P., Masneuf-Pomarède, I. and Dubourdieu, D. 2008. Impact of mixed Torulaspora delbrueckii-Saccharomyces cerevisiae culture on high-sugar fermentation. International Journal of Food Microbiology. 122(3): 312-320.
Caridi, A., Cufari, A., Ramondino, D. 2002. Isolation and clonal pre-selection of enological Saccharomyces. Journal of General Applied Microbiology. 48: 261-267.
Ceccato-Antonini, S., Tosta, C. and da Silva, C. 2004. Determination of yeast killer activity in fermenting sugarcane juice using selected ethanol-making strains. Brazilian Archives Biology and Technology. 47: 13-23.
Ciani, M., Capece, A., Comitini, F., Canonico, L., Siesto, G. and Romano, P. 2016a. Yeast interactions in inoculated wine fermentation. Frontiers in Microbiology. 7(555): 1-7.
Ciani, M., Morales, P., Comitini, F. Tronchioni, J., Canonico, L., Curiel, J., Oro, L., Rodriguez, A. and Gonzalez, R. 2016b. Non-conventional yeast species for lowering ethanol content of wines. Frontiers in Microbiology. 7(642): 1-13.
Combina, M., Elia, A., Mercado, L., Catania, C., Ganga, A. and Martinez, C. 2005. Dynamics of indigenous yeast populations during spontaneous fermentation of wines from Mendoza, Argentina. International Journal of Food Microbiology. 99: 237-243.
D. O. F. 1998. Norma Oficial Mexicana NOM-070-SCFI-1994, Bebidas alcohólicas-mezcal-especificaciones de elaboración, envasado y etiquetado. [consultado 07 febrero de 2020]. Available from: http://www.colpos.mx/bancodenormas/ noficiales/NOM-070-SCFI-1994.PDF.
D. O. F. 2004. Norma Oficial Mexicana NOM-006-SCFI-2004, Bebidas alcohólicas-tequila-especificaciones de elaboración, envasado y etiquetado. [consultado 07 febrero de 2020]. Available from: https://www.crt.org.mx/images/ Documentos/NOM-006-SCFI-2005.pdf.
D. O. F. 2005. Norma Oficial Mexicana NOM-168-SCTI-2004, Bebidas alcohólicas-Bacanora-especificaciones de elaboración, envasado y etiquetado. [consultado 07 febrero de 2020]. Available from: https://dof.vlex.com.mx/vid/scfi-alcoholicas-bacanora-envasado-28151559
De la Cruz, C. 2003. Sembrar maguey y producir Bacanora en el rancho. Revista Rancho. 11: 22-23.
Di Serio, M., Aramo, P., E. de Alteris, Tesser, R. and Santacesaria, E. 2003. Quantitative analysis of the key factors affecting yeast arowth. Industrial and Engineering Chemistry Research. 42 (21): 5109-5116.
Díaz-Montaño, D., Délia, M., Estarrón-Espinoza, M. and Strehaiano P. 2008. Fermentative capability and aroma compound production by yeast strains isolated from Agave tequilana Weber juice. Enzyme and Microbial Technology. 42: 608-616.
Esteve-Zarzoso, B., Peris-Torán, M., García-Maiquez, E., Uruburu, F. and Querol, A. 2001. Yeast populations dynamics during the fermentation and biological aging of sherry wines. Applied Environmental and Microbiology. 67(5): 2056-2061.
Fiore, C., Arrizon, J., Gschaedler, A., Flores, J, and Romano, P. 2005. Comparison between yeasts from grape and agave must for traits of technological interest. World Journal of Microbiology and Biotechnology. 22: 1141-1147.
Fleet, G. 2008. Wine yeast for the future. FEMS Yeast Research. 8(7), 979-995.
Frazier, W. and Westhoff, D. 1998. Microbiología de los Alimentos. España. Ed. Acribia, S.A.
García, M., Esteve-Zarzoso, B., Crespo, J., Cabellos, J. and Arroyo, T. 2017. Yeast monitoring of wine mixed or sequential fermentations made by native strains from D.O “Vinos de Madrid” using Real-Time quantitative PCR. Frontiers in Microbiology. 8(2520): 1-15.
García-Galaz, A., Pérez-Morales, R., Díaz-Cinco, M. and Acedo- Félix, E. 2004. Resistance of Enterococcus strains isolated from pigs to gastrointestinal tract and antagonistic effect against Escherichia coli K88. Revista Latinoamerican de Microbiología. 34: 5-11.
Gschaedler, M., Ramirez, J., Díaz, D., Herrera, J., Arellano, M., Arrizón, L. and Pinal, L. 2004. CIATEJ. Fermentación etapa clave en la elaboración de tequila, Chapter 4. In Ciencia y tecnología del Tequila Avances y Perspectivas. Pp. 62-120. In: Gschaedler MAC (ed) Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Guadalajara, Jalisco, México.
Guillamón, J., Sabate, J. and Barrio, E. 1998. Rapid Identification of wine yeast species based on RFLP analysis of the ribosomal internal transcribed spacer (ITS) region. Archives of Microbiology. 169: 387-392.
Heard, G., and Fleet, G. 1985. Growth of natural yeast flora during the fermentation of inoculated wines. Applied and Environmental Microbiology. 50(3): 727-728.
Lachance, M. 1995. Yeast communities in a natural tequila fermentation. Journal of Antonie Van Leeuwenhoek. 68: 151-160.
Lopez-Alvarez, A., Díaz-Pérez, A., Sosa-Aguirre, C., Macías- Rodríguez, L., Campos-García, J. 2012. Ethanol yield and
volatile compound content in fermentation of agave must by Kluyveromyces marxianus UMPe-1 comparing with Saccharomyces cerevisiae baker’s yeast used in tequila production. Journal of Bioscience and Bioengineering. 113 (5): 614-618.
Mancilla-Margalli, N.D. and Lopez M. 2006. Water-Soluble Carbohydrates and fructan structure patterns from Agave and Dasylirion species. Journal of Agricultural and Food Chemestry. 54: 7832−7839
Mas, A., Torija, M., Beltrán, G., Novo, M., Hierro, N., Poblet, M., Rozés, N. and Guillamón, J. 2002. Selección de Levaduras. Tecnología del Vino, Fermentos. 39-44. http://www.alcion.es
Miller, G. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry. 31: 426-428.
Moreno, S. 1998. Agave angustifolia. La bacanora desde su origen hasta nuestros días. Una bebida sonorense con calidad internacional. Instituto del medio ambiente y el desarrollo de Sonora (Imades). Revista Entorno. 2: 3-5.
Núñez, L. 2001. La producción de mezcal Bacanora. Centro de Investigación en Alimentación y Desarrollo, A.C. Hermosillo, Sonora, México.
Nolasco-Cancino, H., Santiago-Urbina, J., Wacher, C. and Ruíz- Teran, F. 2018. Predominant yeasts during artisanal mezcal fermentation and their capacity to ferment maguey juice. Frontiers in Microbiology. 9(2900):1-12.
Querol, A., Fernández-Espinar, M., del Olmo M. and Barrio, E. 2003. Adaptative evolution of wine yeast. International Journal of Food Microbiology. 86: 3-10.
Rodríguez, L., Abad, D., Gómez, J., Casanova, J. and Lema, C. 1998. Fenotipo killer: Distribución en la comarca de la Ribiera sacra en las poblaciones de Saccharomyces cerevisiae. Ciencia y Tecnología de Alimentos. 2: 33-37.
Romano, P., Fiore, C., Paraggio, M., Caraso, M. and Capece, A. 2003. Function of yeast species and strains in wine flavor. International Journal of Food Microbiology. 86: 169-180.
Segura-García, L., Taillandier P., Brandam C. and Gschaedler, A. Fermentative capacity of Saccharomyces and non- Saccharomyces in agave juice and semi-synthetic medium. 2015. Food Science and Technology, 60 (1): 284-291.
Walker, G., M. and Hill, A. 2016. Saccharomyces cerevisiae in the production of whisk(e)y. Beverages. 2(38): 1-15.
Wondra, M. and Berovic, M. 2001. Analyses of aroma components of Chardonnay wine fermented by different yeast strains. Food Technology and Biotechnology. 39(2): 141-148.
Yanez, G. 2003. El maguey de Bacanora (Agave angustifolia). Instituto del medio ambiente y el desarrollo de Sonora (Imades). Revista Entorno. 11: 5-11
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